LOCUS       40924_10631        8786 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCHC01, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8786)
  AUTHORS   Chen C-H., DeMay BS, Gladfelter AS, Dunlap JC, Loros JJ.
  TITLE     Direct interaction between VVD and WCC regulates photoadaptation in 
            Neurospora
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8786)
  AUTHORS   Chen C-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2010) Genetics, Dartmouth Medical School, 702 
            Remsen, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 8786)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8786)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-MAR-2010) Genetics, Dartmouth Medical School, 702 Remsen, 
            Hanover, NH 03755, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8786
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        196..416
                     /label=URA3 promoter
     CDS             417..1217
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     rep_origin      complement(1351..1806)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     misc_recomb     1957..3025
                     /note="derived from Neurospora crassa csr-1; Neurospora 
                     homologous recombination site"
     misc_recomb     3026..3054
                     /label=yeast homologous recombination site
                     /note="yeast homologous recombination site"
     promoter        3055..3072
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_recomb     3085..3113
                     /label=yeast homologous recombination site
                     /note="yeast homologous recombination site"
     CDS             complement(3264..3812)
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     promoter        complement(3835..4194)
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     misc_recomb     4206..5220
                     /note="derived from Neurospora crassa csr-1; Neurospora 
                     homologous recombination site"
     protein_bind    complement(5247..5263)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5271..5301)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5316..5337)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5625..6213)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6387..7244)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7245..7349)
                     /label=AmpR promoter
     rep_origin      complement(7376..8718)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     1921 taacgccagg gttttcccag tcacgacgat ttaaattaga tccaatctga gcctcgcggt
     1981 gatgattgcc agacgggcgg gatgctgtgg cgacttggtg gtttttgttc gggctgatcg
     2041 ctttcagggc gacgtttcac ggtcttgtcg ggatcagatg gacattgagg tggcctagcg
     2101 tcagggtgcc gctgagcgct gacctcaact gacgccgtcc aaggccgacc cctgcgcttg
     2161 acccaacacc ctgacaccaa gaagccagtg gaacccagga ggtctgccaa gactttgaaa
     2221 gcttggcacg agcgtctcag ctctttgaag cctgagtttt caggcaagaa ttctgcttgt
     2281 aattgccggg ccggccgaca acgcggggcg gcgggagggg acgctcggcc gccggagctc
     2341 gcttccatgc attttggatg gagtaaaaat tgttaaacac ccgacccgac tccgttctcc
     2401 ctctcgtctc aggtctttgt tgtgcatcca tcgccgtgta gcagatttcc atcgttcgtt
     2461 tgttcggact ttaccttgta cttttagctt cattatcatc ctacaatcat ccaggatttc
     2521 atcttccaca accggtacgt tatcattttt acttttgttg ccatgttctt cttgagcctt
     2581 ttctgtctag aggagctctc cctgccggct gggagcttgc ccaaagctcc aagctctatc
     2641 tgtggcagag ctctgagagc ttccattccc gggatttctt ggcatcagat tcaattcgaa
     2701 tgtccttttt tccatccacg ctcgagatga ggggtgaggt ttgttagttt gacaatacca
     2761 ccaccgcctt ttggtcccta ccccagacat ggccatcaag gtcgttaatg ccgtccatcc
     2821 ctcttgttcg gcttggggta tcgtccatgg ctccgcaatg tcgtcaatgg ctgcattgac
     2881 agagggggac tccaaatgaa agaggcaatc gcaacgctat gaagacccaa caagccatat
     2941 gatggagcca tgggtctacg gtcctttggc ccggatgtct ttggatagct cttgccagcg
     3001 ctttagctgc caactataca cacaccggaa ttatacgatt taggtgactg caggattaac
     3061 cctcactaaa gggactagtc ctcactagag gatccgacac tatagaatac ctattctcgg
     3121 tactatgcat tacatgttaa ttattacatg cttaacgtaa ttcaacagaa attatatgat
     3181 aatcatcgca agaccggcaa caggattcaa tcttaagaaa ctttattgcc aaatgtttga
     3241 acgatctgca ggtcgacgga tcagatctcg gtgacgggca ggaccggacg gggcggtacc
     3301 ggcaggctga agtccagctg ccagaaaccc acgtcatgcc agttcccgtg cttgaagccg
     3361 gccgcccgca gcatgccgcg gggggcatat ccgagcgcct cgtgcatgcg cacgctcggg
     3421 tcgttgggca gcccgatgac agcgaccacg ctcttgaagc cctgtgcctc cagggacttc
     3481 agcaggtggg tgtagagcgt ggagcccagt cccgtccgct ggtggcgggg ggagacgtac
     3541 acggtcgact cggccgtcca gtcgtaggcg ttgcgtgcct tccaggggcc cgcgtaggcg
     3601 atgccggcga cctcgccgtc cacctcggcg acgagccagg gatagcgctc ccgcagacgg
     3661 acgaggtcgt ccgtccactc ctgcggttcc tgcggctcgg tacggaagtt gaccgtgctt
     3721 gtctcgatgt agtggttgac gatggtgcag accgccggca tgtccgcctc ggtggcacgg
     3781 cggatgtcgg ccgggcgtcg ttctgggctc atggttactt cctaatcgaa gctttgcttg
     3841 ggtagaatag gtaagtcaga ttgaatctga aataaaggga ggaagggcga acttaagaag
     3901 gtatgaccgg gtcgttcact taccttgctt gacaaacgca ccaagttatc gtgcaccaag
     3961 cagcagatga taataatgtc ctcgttcctg tctgctaata agagtcacac ttcgagcgcc
     4021 gccgctactg ctacaagtgg ggctgatctg accagttgcc taaatgaacc atcttgtcaa
     4081 acgacacaaa ttttgtgctc accgcctgga cgactaaacc aaaataggca ttcattgttg
     4141 acctccacta gctccagcca agcccaaaaa gtgctccttc aatatcatct tctgtcgagt
     4201 ctagatgact tgttcctgta cctcgtcacc atccagccac cgtctgaaat agacgcagtt
     4261 gctggggctg acaggtccgc cttcctcgca catatcatag acagggcacc gcccacaagg
     4321 cgcctccata aggccacttg cactcggctc aactcccata aatgaggtaa caatgcctcc
     4381 tttggttgga tcatggtcga ggcgtcgttg attgcacgtc ctcggatcct gtttggggat
     4441 gcgcgtcaca cggtacccct tgcgcccatt gaccttgaca ggctctacta agccatcgta
     4501 gtatagcaca tccaccaact gctggatatc cgcctcgctc aacgtcgtat tattcgtgat
     4561 gccggcgtgg tggatgaact ttgcaatgtc cgacacagtc gggtacgact tgtaaccggc
     4621 cgggagaggc agcaattgcg gcttgccggg cctggtcttc ttggccggtg ccggtgccgc
     4681 cgtgtcgtcg ttggaaatct cgcccgcgct gcgcttcgtc cccttggaaa tatcctccga
     4741 gtggggaatg gtcccctgaa ccacgccctt cttgggattc ttttcacgct gctgtgctcc
     4801 tcctccttgt gctcctactc ctggtactcc agcaccgccg ccgtgggaag tatatgtcga
     4861 tttctgcttg ataaagtcaa acacaatttc ctgtagctcg ttgatgaagg ccgtgtcgag
     4921 ctcgccctca gtaaaccagg ggccacctgt ggcgcgatcg gagggacgta ggttcgcctt
     4981 gatgtacatc ttcttgttgg ggtgctcgac gttcttcatg ctggcgatgt agcccttttg
     5041 ctcgagatac ttgatgcaat gcttcataag cgagtcggcc atgttgagct tgttcttgat
     5101 ggtccggttc cagatgccgt cggccccggc ttcgtcgacc atggcgtata ccatcattat
     5161 ttcggagttg gggagggagg tgtacctttt gggaggaagg ggaggttagt tggtgtgctc
     5221 atttaaatgc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatagga
     5281 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgaggtaact cacattaatt
     5341 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     5401 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     5461 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     5521 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     5581 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     5641 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     5701 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     5761 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     5821 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     5881 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     5941 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     6001 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     6061 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     6121 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6181 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     6241 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     6301 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     6361 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     6421 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     6481 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     6541 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     6601 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     6661 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     6721 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     6781 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     6841 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     6901 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     6961 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     7021 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     7081 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     7141 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     7201 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     7261 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     7321 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgaacga
     7381 agcatctgtg cttcattttg tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac
     7441 aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc tattttacca
     7501 acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc gctaattttt
     7561 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgag agcgctattt
     7621 taccaacaaa gaatctatac ttcttttttg ttctacaaaa atgcatcccg agagcgctat
     7681 ttttctaaca aagcatctta gattactttt tttctccttt gtgcgctcta taatgcagtc
     7741 tcttgataac tttttgcact gtaggtccgt taaggttaga agaaggctac tttggtgtct
     7801 attttctctt ccataaaaaa agcctgactc cacttcccgc gtttactgat tactagcgaa
     7861 gctgcgggtg cattttttca agataaaggc atccccgatt atattctata ccgatgtgga
     7921 ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga ttcttcattg gtcagaaaat
     7981 tatgaacggt ttcttctatt ttgtctctat atactacgta taggaaatgt ttacattttc
     8041 gtattgtttt cgattcactc tatgaatagt tcttactaca atttttttgt ctaaagagta
     8101 atactagaga taaacataaa aaatgtagag gtcgagttta gatgcaagtt caaggagcga
     8161 aaggtggatg ggtaggttat atagggatat agcacagaga tatatagcaa agagatactt
     8221 ttgagcaatg tttgtggaag cggtattcgc aatattttag tagctcgtta cagtccggtg
     8281 cgtttttggt tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa agcgctctga
     8341 agttcctata ctttctagag aataggaact tcggaatagg aacttcaaag cgtttccgaa
     8401 aacgagcgct tccgaaaatg caacgcgagc tgcgcacata cagctcactg ttcacgtcgc
     8461 acctatatct gcgtgttgcc tgtatatata tatacatgag aagaacggca tagtgcgtgt
     8521 ttatgcttaa atgcgtactt atatgcgtct atttatgtag gatgaaaggt agtctagtac
     8581 ctcctgtgat attatcccat tccatgcggg gtatcgtatg cttccttcag cactaccctt
     8641 tagctgttct atatgctgcc actcctcaat tggattagtc tcatccttca atgctatcat
     8701 ttcctttgat attggatcat actaagaaac cattattatc atgacattaa cctataaaaa
     8761 taggcgtatc acgaggccct ttcgtc
//
