pCGL0609 vector (V008539)

LOCUS       40924_10581        6398 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pCGL0609, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6398)
  AUTHORS   Reyes O, Guyonvarch A, Bonamy C, Salti V, David F, Leblon G.
  TITLE     'Integron'-bearing vectors: a method suitable for stable chromosomal
            integration in highly restrictive corynebacteria
  JOURNAL   Gene 107 (1), 61-68 (1991)
  PUBMED    1660430
REFERENCE   2  (bases 1 to 6398)
  AUTHORS   Ankri S, Reyes O, Leblon G.
  TITLE     Electrotransformation of highly DNA-restrictive corynebacteria with 
            synthetic DNA
  JOURNAL   Plasmid 35 (1), 62-66 (1996)
  PUBMED    8693028
REFERENCE   3  (bases 1 to 6398)
  AUTHORS   Ben-Samoun K, Leblon G, Reyes O.
  TITLE     Positively regulated expression of the Escherichia coli araBAD 
            promoter in Corynebacterium glutamicum
  JOURNAL   FEMS Microbiol. Lett. 174 (1), 125-130 (1999)
  PUBMED    10234830
REFERENCE   4  (bases 1 to 6398)
  AUTHORS   Ben-Samoun K, Leblon G, Reyes O.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-SEP-1998) Laboratoire de Biologie Moleculaire des 
            Corynebacteries, Institut de Genetique et Microbiologie URA 2225, 
            Universite de Paris XI, Orsay F-91405, France
REFERENCE   5  (bases 1 to 6398)
  TITLE     Direct Submission
REFERENCE   6  (bases 1 to 6398)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1991"; volume: "107"; issue: "1"; pages: "61-68"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Plasmid"; 
            date: "1996"; volume: "35"; issue: "1"; pages: "62-66"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "FEMS 
            Microbiol. Lett."; date: "1999"; volume: "174"; issue: "1"; pages: 
            "125-130"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: "Submitted 
            (17-SEP-1998) Laboratoire de Biologie Moleculaire des 
            Corynebacteries, Institut de Genetique et Microbiologie URA 2225, 
            Universite de Paris XI, Orsay F-91405, France"
COMMENT     SGRef: number: 5; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     72..88
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             675..1466
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(2411..3799)
                     /gene="repBl1"
                     /label=repBl1
     CDS             complement(2495..3700)
                     /codon_start=1
                     /gene="repBl1"
                     /product="replicase"
                     /label=repBl1
                     /note="minimally known pBl1 region able to promote
                     replication"
                     /protein_id="AAD08692.1"
                     /translation="MYKITNSKALAGCHRWRRDEAVAVSWSSNGASQFEGLQNSHSRWG
                     SPLAELEVMGERRIELAIATKNHLAAGGALMMFVGTVRHNRSQSFAQVEAGIKTAYSSM
                     VKTSQWKKERARYGVEHTYSDYEVTDSWANGWHLHRNMLLFLDRPLSDDELKAFEDSMF
                     SRWSAGVVKAGMDAPLREHGVKLDQVSTWGGDAAKMATYLAKGMSQELTGSATKTASKG
                     SYTPFQMLDMLADQSDAGEDMDAVLVARWREYEVGSKNLRSSWSRGAKRALGIDYIDAD
                     VRREMEEELYKLAGLEAPERVESTRVAVALVKPDDWKLIQSDFAVRQYVLDCVDKAKDV
                     AAAQRVANEVLASLGVDSTPCMIVMDDVDLDAVLPTHGDATKRDLNAAVFAGNEQTILR
                     TH"
     rep_origin      complement(5287..5821)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     rep_origin      5889..6317
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"