LOCUS       40924_10581        6398 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pCGL0609, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6398)
  AUTHORS   Reyes O, Guyonvarch A, Bonamy C, Salti V, David F, Leblon G.
  TITLE     'Integron'-bearing vectors: a method suitable for stable chromosomal
            integration in highly restrictive corynebacteria
  JOURNAL   Gene 107 (1), 61-68 (1991)
  PUBMED    1660430
REFERENCE   2  (bases 1 to 6398)
  AUTHORS   Ankri S, Reyes O, Leblon G.
  TITLE     Electrotransformation of highly DNA-restrictive corynebacteria with 
            synthetic DNA
  JOURNAL   Plasmid 35 (1), 62-66 (1996)
  PUBMED    8693028
REFERENCE   3  (bases 1 to 6398)
  AUTHORS   Ben-Samoun K, Leblon G, Reyes O.
  TITLE     Positively regulated expression of the Escherichia coli araBAD 
            promoter in Corynebacterium glutamicum
  JOURNAL   FEMS Microbiol. Lett. 174 (1), 125-130 (1999)
  PUBMED    10234830
REFERENCE   4  (bases 1 to 6398)
  AUTHORS   Ben-Samoun K, Leblon G, Reyes O.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-SEP-1998) Laboratoire de Biologie Moleculaire des 
            Corynebacteries, Institut de Genetique et Microbiologie URA 2225, 
            Universite de Paris XI, Orsay F-91405, France
REFERENCE   5  (bases 1 to 6398)
  TITLE     Direct Submission
REFERENCE   6  (bases 1 to 6398)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1991"; volume: "107"; issue: "1"; pages: "61-68"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Plasmid"; 
            date: "1996"; volume: "35"; issue: "1"; pages: "62-66"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "FEMS 
            Microbiol. Lett."; date: "1999"; volume: "174"; issue: "1"; pages: 
            "125-130"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: "Submitted 
            (17-SEP-1998) Laboratoire de Biologie Moleculaire des 
            Corynebacteries, Institut de Genetique et Microbiologie URA 2225, 
            Universite de Paris XI, Orsay F-91405, France"
COMMENT     SGRef: number: 5; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     72..88
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             675..1466
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(2411..3799)
                     /gene="repBl1"
                     /label=repBl1
     CDS             complement(2495..3700)
                     /codon_start=1
                     /gene="repBl1"
                     /product="replicase"
                     /label=repBl1
                     /note="minimally known pBl1 region able to promote
                     replication"
                     /protein_id="AAD08692.1"
                     /translation="MYKITNSKALAGCHRWRRDEAVAVSWSSNGASQFEGLQNSHSRWG
                     SPLAELEVMGERRIELAIATKNHLAAGGALMMFVGTVRHNRSQSFAQVEAGIKTAYSSM
                     VKTSQWKKERARYGVEHTYSDYEVTDSWANGWHLHRNMLLFLDRPLSDDELKAFEDSMF
                     SRWSAGVVKAGMDAPLREHGVKLDQVSTWGGDAAKMATYLAKGMSQELTGSATKTASKG
                     SYTPFQMLDMLADQSDAGEDMDAVLVARWREYEVGSKNLRSSWSRGAKRALGIDYIDAD
                     VRREMEEELYKLAGLEAPERVESTRVAVALVKPDDWKLIQSDFAVRQYVLDCVDKAKDV
                     AAAQRVANEVLASLGVDSTPCMIVMDDVDLDAVLPTHGDATKRDLNAAVFAGNEQTILR
                     TH"
     rep_origin      complement(5287..5821)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     rep_origin      5889..6317
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gctaaccgtt tttatcaggc tctgggaggc agaataaatg atccgtcgag ctccaccgcg
       61 gtggcggccg ctctagaact agtggatccc ccgggctgca ggcctcgaga tctccatgga
      121 cgcgtgacgt cgacggtatc gataaaccca gcgaaccatt tgaggtgata ggtaagatta
      181 taccgaggta tgaaaacgag aattggacct ttacagaatt actctatgaa gcgccatatt
      241 taaaaagcta ccaagacgaa gaggatgaag aggatgagga ggcagattgc cttgaatata
      301 ttgacaatac tgataagata atatatcttt tatatagaag atatcgccgt atgtaaggat
      361 ttcagggggc aaggcatagg cagcgcgctt atcaatatat ctatagaatg ggcaaagcat
      421 aaaaacttgc atggactaat gcttgaaacc caggacaata accttatagc ttgtaaattc
      481 tatcataatt gtggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttcaa
      541 aacaactttg aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa
      601 ttggagttcg tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag
      661 gaaggaaata ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa
      721 aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg
      781 gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg
      841 atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg
      901 tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg
      961 tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg
     1021 cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct
     1081 tagacagccg cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt
     1141 gcgaaaactg ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa
     1201 agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca
     1261 tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg
     1321 acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac
     1381 agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat
     1441 attatatttt actggatgaa ttgttttagt acctagattt agatgtctaa aaagctttaa
     1501 ctacaagctt tttagacatc taatcttttc tgaagtacat ccgcaactgt ccatactctg
     1561 atgttttata tcttttctaa aagttcgcta gataggggtc ccgagcgcct acgaggaatt
     1621 tgtatcgatg ataagctgtc aaacatgaga attacaactt atatcgtatg gggctgactt
     1681 caggtgctac atttgaagag ataaattgca ctgaaatcta gagcggccgc caccgcggtg
     1741 gagctcgacg gatcagatca tatcgtcaat tattacctcc acggggagag cctgagcaaa
     1801 ctggcctcag gcatttgaga agcacacggt cacactgctt ccggtagtca ataaaccggt
     1861 aaaccagcaa tagacataag cggctattta acgaccctgc cctgaaccga cgaccgggtc
     1921 gaatttgctt tcgaatttct gccattcatc cgcttattat cacttattca ggcgtagcac
     1981 caggcgttta agggcaccaa taactgcctt aaaaaaatta cgccccgccc tgccactcat
     2041 cgcagtaacg tcaacaacca cccccgcagg cgttaagttg ccccgccaac agaaaggaaa
     2101 ccaacacgaa acaacaacac aaaaaggttt cacagaaaaa agcgtatgcg ctaacgtatg
     2161 ccccgcagca cggcaaaacg cgcgttaagc cctagcccac gcgcgcgtag gtattactca
     2221 tgcccactat ggtgtgcaca ctgcccacta cggtgtgcaa tctattcacg atgccacccc
     2281 cagatacagt gaagccccgc caatccgaac tagatcagat caacggggca acccattgtc
     2341 cccagctttg attaggagcc aggcacataa cagcatgaca gttccattcc tgatgaaatc
     2401 agccattgtc aacaacaaga cccatcatag tttgcccccg cgacattgac cataaattca
     2461 tcgcacaaaa tatcgaacgg ggtttatgcc gcttttagtg ggtgcgaaga atagtctgct
     2521 cattacccgc gaacaccgcc gcattcagat cacgcttagt agcgtcccca tgagtaggca
     2581 gaaccgcgtc caagtccaca tcatccataa cgatcatgca cggggtggaa tccacaccca
     2641 gacttgccag cacctcatta gcgacacgtt gcgcagcggc cacgtcctta gccttatcca
     2701 cgcaatctag aacgtactgc ctaaccgcga aatcagactg aatcagtttc caatcatcgg
     2761 gcttcaccaa agcaacagca acgcgggttg attcgacccg ttccggtgct tccagaccgg
     2821 cgagcttgta cagttcttct tccatttcac gacgtacatc agcgtctatg taatcaatgc
     2881 ccaaagcacg cttagcccca cgtgaccagg acgaacgcag gtttttagaa ccaacctcat
     2941 actcacgcca ccgagccacc aaaacagcgt ccatatcctc gccggcgtcg ctttgatcgg
     3001 ccaacatatc caacatctga aacggcgtgt acgacccctt agacgcggtt ttagtagcgg
     3061 agccagtcag ttcctgagac atgcccttag cgaggtaggt tgccattttc gcagcgtctc
     3121 caccccaggt agacacctga tcaagtttga ccccgtgctc acgcagtggc gcgtccatac
     3181 cggccttaac cacaccagca gaccagcggg aaaacatgga atcctcaaac gccttgagtt
     3241 catcgtcaga cagtggacga tccaagaaca acagcatgtt gcggtgcaag tgccaaccgt
     3301 tcgcccaaga gtctgtgacc tcatagtcac tataggtgtg ctccaccccg taccgtgcac
     3361 gttctttctt ccactgagat gttttcacca tcgaagagta cgcagtctta atacccgctt
     3421 caacctgcgc aaatgactgt gagcggttgt gtcgaacagt gcccacaaac atcatgagcg
     3481 cgccacccgc cgccaagtga ttcttagtag caatagccag ctcaatgcgg cgttcgccca
     3541 tgacttccaa ttcagccaga ggtgaccccc agcgagagtg agagttttgc agaccctcaa
     3601 actgcgaagc accgttagac gaccaggaca ccgcaacagc ttcgtccctg cgccacctat
     3661 ggcaccccgc cagagcctta ctattggtga tcttgtacat gacgttttgc ctacgccacg
     3721 ccctagcgcg agtgacctta gaaccctcat tgacctgcgg ttccttagag gtgttcactt
     3781 ctatttcagt gttacctaga cccgatgttg tgcggggttg cgcagtgcga gtttgtgcgg
     3841 gtgttgtgcc cgttgtctta gctagtgcta tggttgtcaa ttgaaacccc ttcgggttat
     3901 gtggcccccg tgcatatgag ttggtagctc gcacgggggt ttgtcttgtc tagggactat
     3961 taatttttag tggtgtttgg tggccgccta gcttggctat gcgtgccagc ttacccgtac
     4021 tcaatgttaa agatttgcat cgacatggga gggttacgtg tccgatacct agggggggta
     4081 tccgcgacta ggtgccccgg tgctcactgt ctgtaccggc ggggcaagcc ccacaccccg
     4141 catggacagg gtggctccgc cccctgcacc cccagcaatc tgcatgtaca tgttttacac
     4201 attagcacga catgactgca tgtgcatgca ctgcatgcag actaggtaaa tatgagtatg
     4261 tacgactagt aacaggagca ctgcacataa tgaatgagtt gcaggacaat gtttgctacg
     4321 catgcgcatg acatatcgca ggaaagctac tagagtctta aagcatggca accaaggcac
     4381 agctagaaca gcaactacaa gaagctcaac aggcactaca ggcgcagcaa gcgcaggcac
     4441 aagccaccat cgaagcacta gaagcgcagg caaaggctaa gcccgtcgtg gtcaccgcac
     4501 gcgttccttt ggcactacgt gaggacatga agcgcgcagg catgcagaac ggtgaaaacc
     4561 tccaagagtt catgatcgcc gcgtttaccg agcggctaga aaagctcacc accaccgaca
     4621 acgaggaaaa caatgtctaa cccactagtt ctctttgccc accgtgaccc ggtaaatgac
     4681 gtgacgttcg agtgcattga gcacgccacc tacgacacac tttcacacgc taaagaccag
     4741 atcaccgccc aaatgcaagc cctagacgaa gaagccgccc tactgcccta atgggtgttt
     4801 catgggtgtt tccctagtgt ttcatggtgt tttcacctaa gctagggaat tgcgcgagaa
     4861 gtctcgcaaa aatcagcaac ccccggaacc acacagttca cgggggttct tctatgccag
     4921 aaatcagaaa ggggaaccag tgaacgaccc cgaatctgtc cctcctgttc agctactgac
     4981 ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc gctagcggag tgtatactgg
     5041 cttactatgt tggcactgat gagggtgtca gtgaagtgct tcatgtggca ggagaaaaaa
     5101 ggctgcaccg gtgcgtcagc agaatatgtg atacaggata tatttccgct tcctcgctca
     5161 ctgactcgct acgctcggtc gttcgactgc ggcgagcgga aatggcttac gaacggggcg
     5221 gagatttcct ggaagatgcc aggaagatac ttaacaggga agtgagaggg ccgcggcaaa
     5281 gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca
     5341 gtggtggcga aacccgacag gactataaag ataccaggcg tttcccctgg cggctccctc
     5401 gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt
     5461 ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg
     5521 tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga
     5581 gtccaacccg gaaagacatg caaaagcacc actggcagca gccactggta attgatttag
     5641 aggagtcttg aagtcatgcg ccggttaagg ctaaaggaca agttttggtg actgcgctcc
     5701 tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc
     5761 ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca
     5821 agaagatcat cttattaatc agataaaata ttaacgttta caatttccca ttcgccatgg
     5881 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     5941 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     6001 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     6061 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     6121 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     6181 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     6241 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     6301 aaaatttaac gcgaatttta acaaaatctg tccctcctgt tcagctactg acggggtggt
     6361 gcgtaacggc aaaagcaccg ccggacatca gcgctagc
//
