pCG6-attB vector (V008547)

LOCUS       V008547                 6431 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008547
VERSION     V008547
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6431)
  AUTHORS   Nkrumah LJ, Muhle RA, Moura PA, Ghosh P, Hatfull GF, Jacobs WR Jr.,
            Fidock DA.
  TITLE     Efficient site-specific integration in Plasmodium falciparum
            chromosomes mediated by mycobacteriophage Bxb1 integrase
  JOURNAL   Nat. Methods 3 (8), 615-621 (2006)
   PUBMED   16862136
REFERENCE   2  (bases 1 to 6431)
  AUTHORS   Nkrumah LJ, Fidock DA.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2006) Microbiology and Immunology, Albert Einstein
            College of Medicine, 1300 Morris Park, Bronx, NY 10461, USA
REFERENCE   3  (bases 1 to 6431)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6431)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat.
            Methods"; date: "2006"; volume: "3"; issue: "8"; pages: "615-621"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (25-JUN-2006) Microbiology and Immunology, Albert Einstein College
            of Medicine, 1300 Morris Park, Bronx, NY 10461, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        626..644
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    657..2443
                     /label="contains promoter and 5' UTR from hrp3 gene"
                     /note="contains promoter and 5' UTR from hrp3 gene"
     primer_bind     674..690
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             2444..3004
                     /gene="DHFR"
                     /label="Dihydrofolate reductase"
                     /note="Dihydrofolate reductase from Homo sapiens.
                     Accession#: P00374"
     regulatory      3012..3579
                     /label="derived from hrp2 3' UTR"
                     /note="derived from hrp2 3' UTR"
                     /regulatory_class="terminator"
     misc_feature    3588..4160
                     /gene="cg6"
                     /note="similar to Plasmodium falciparum glutaredoxin-like
                     protein; truncated fragment lacking 5' and 3' ends of
                     coding sequence"
     gene            3588..4160
                     /gene="cg6"
                     /label="cg6"
     misc_recomb     4167..4210
                     /label="attB"
                     /note="attB"
     promoter        complement(4243..4261)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4282..4298)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4306..4322)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4330..4360)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4375..4396)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4684..5272)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5446..6303)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6304..6408)
                     /label="AmpR promoter"