LOCUS       V008547                 6431 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008547
VERSION     V008547
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6431)
  AUTHORS   Nkrumah LJ, Muhle RA, Moura PA, Ghosh P, Hatfull GF, Jacobs WR Jr.,
            Fidock DA.
  TITLE     Efficient site-specific integration in Plasmodium falciparum
            chromosomes mediated by mycobacteriophage Bxb1 integrase
  JOURNAL   Nat. Methods 3 (8), 615-621 (2006)
   PUBMED   16862136
REFERENCE   2  (bases 1 to 6431)
  AUTHORS   Nkrumah LJ, Fidock DA.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2006) Microbiology and Immunology, Albert Einstein
            College of Medicine, 1300 Morris Park, Bronx, NY 10461, USA
REFERENCE   3  (bases 1 to 6431)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6431)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat.
            Methods"; date: "2006"; volume: "3"; issue: "8"; pages: "615-621"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (25-JUN-2006) Microbiology and Immunology, Albert Einstein College
            of Medicine, 1300 Morris Park, Bronx, NY 10461, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        626..644
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    657..2443
                     /label="contains promoter and 5' UTR from hrp3 gene"
                     /note="contains promoter and 5' UTR from hrp3 gene"
     primer_bind     674..690
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             2444..3004
                     /gene="DHFR"
                     /label="Dihydrofolate reductase"
                     /note="Dihydrofolate reductase from Homo sapiens.
                     Accession#: P00374"
     regulatory      3012..3579
                     /label="derived from hrp2 3' UTR"
                     /note="derived from hrp2 3' UTR"
                     /regulatory_class="terminator"
     misc_feature    3588..4160
                     /gene="cg6"
                     /note="similar to Plasmodium falciparum glutaredoxin-like
                     protein; truncated fragment lacking 5' and 3' ends of
                     coding sequence"
     gene            3588..4160
                     /gene="cg6"
                     /label="cg6"
     misc_recomb     4167..4210
                     /label="attB"
                     /note="attB"
     promoter        complement(4243..4261)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4282..4298)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4306..4322)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4330..4360)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4375..4396)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4684..5272)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5446..6303)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6304..6408)
                     /label="AmpR promoter"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtacccc
      661 cagtcacgac gttgtaaaac gacggccagt gaattcgagc tcgcccaatc attattttat
      721 gttaaaaatt taaagaaaat ttattttaat ttttttctaa aaaaattttt cttcaacttt
      781 ttaatttatt tatttatttc tatattttaa acaaattata taaatatatt tcaaattatt
      841 ctccttacat ttttaaaaaa tttaaatcat aattctattc atatacaagc tatttttatt
      901 attttttagt tattttattg tggtattcag aacattcgga tttattattc gtataccaat
      961 aatttatttt attttatttt tttttctctt aatataaata taaatgaata tattcataat
     1021 tataatattt tttatattat attcctctct atttaagagt atataaatat gtataataaa
     1081 tttatataat atatataata attagttaat aaaaatataa attaataatc tgaaaaatac
     1141 ataaaacgtt tttatctata tttatacaca tataatataa tacatattta tctataatat
     1201 agatattata tattctttta gaatatttat atatataata tattatgttc caaaaaaaat
     1261 gtatattatt taattacaaa ttatttttat attttatgga aaaaaaaaaa acgtgttata
     1321 tatgtatatg tatgtatttt aaaatataat aaaatgaaat accataaaaa attatttttc
     1381 agtcaatata atgttattat tctttaatac aaattttaaa agaaaattta tttttttatt
     1441 taattctcat ttttccattt ttaaacattt tatttatatc tctgaaaaaa aatattctat
     1501 ttataatatt taaaggaaat acaaaagata cataagatag atggaatatt cttatattca
     1561 taataatatt caaaaaataa cagatttaaa ccctcaaaaa tatagaaata atatataaat
     1621 atgtgatttc taatgtacac aaaaaaaaaa aaattaaatt ttaagagata taaaaaataa
     1681 aatatttaaa atatttttta ttaaaacaaa tttaaattat tacctttcta cataaaattt
     1741 tttactttaa tttttttatg aatataataa aatttattat tttttaaaaa taaaatctat
     1801 ccatacactt atgctgtatt taaatgtttt tcaaaaaaat attaaagcaa ataaaaaaat
     1861 cattataaat tttaattaaa aaaaaagtct tatattaata aaacaaaata ataaataaag
     1921 taataatcct ttttatataa aattattttt gtttaagtat aaatcctaaa tatttttatt
     1981 tatttattta aataaaaaat ttatttaaaa catttttata aatttttcat caactttgct
     2041 tttaaagttt ctactttttt taataaaaaa aattaactaa ttataaataa ttttgctttt
     2101 ttataaaatt caaggtataa aataattaaa taattttata taatatattt atatatataa
     2161 attgtttata tttatatata tatatatata tatgtgtata tactatatat aagaagaagt
     2221 atataatgaa ttatatataa tgcttatttt tcacattttc atgaatttat attatacaga
     2281 tttttttttt atatatatgc aaaaacttag tattctatat tttatagata ccatatatta
     2341 tataatgaat aatatatata tatatatata tatatatata tatatatata tatatatttt
     2401 tttattattt ctttttttat attaatacag ttattttaaa aaaatgcatg gttcgctaaa
     2461 ctgcatcgtc gctgtgtccc agaacatggg catcggcaag aacggggact acccctggcc
     2521 accgctcagg aacgaattta gatatttcca gagaatgacc acaacctctt cagtagaagg
     2581 taaacagaat ctggtgatta tgggtaagaa gacctggttc tccattcctg agaagaatcg
     2641 acctttaaag ggtagaatta atttagttct cagcagagaa ctcaaggaac ctccacaagg
     2701 agctcatttt ctttccagaa gtctagatga tgccttaaaa cttactgaac aaccagaatt
     2761 agcaaataaa gtagacatgg tctggatagt tggtggcagt tctgtttata aggaagccat
     2821 gaatcaccca ggccatctta aactatttgt gacaaggatc atgcaagact ttgaaagtga
     2881 cacgtttttt ccagaaattg atttggagaa atataaactt ctgccagaat acccaggtgt
     2941 tctctctgat gtccaggagg agaaaggcat taagtacaaa tttgaagtat atgagaagaa
     3001 tgattaagct tatttaataa tagattaaaa atattataaa aataaaaaca taaacacaga
     3061 aattacaaaa aaaatacata tgaatttttt ttttgtaatc ttccttataa atatagaata
     3121 atgaatcata taaaacatat cattattcat ttatttacat ttaaaattat tgtttcagta
     3181 tctttaattt attatgtata tataaaaata acttacaatt ttattaataa acaatatatg
     3241 tttattaatt catgttttgt aatttatggg atagcgattt tttttactgt ctgtattttt
     3301 cttttttaat tatgttttaa ttgtatttta tttttattat tgttcttttt atagtattat
     3361 tttaaaacaa aatgtatttt ctaagaactt ataataataa taaatataaa ttttaataaa
     3421 aattatattt atcttttaca atatgaacat aaagtacaac attaatatat agcttttaat
     3481 atttttattc ctaatcatgt aaatcttaaa tttttctttt taaacatatg ttaaatattt
     3541 atttctcatt atatataaga acatatttat taaactgcag cccgggggat ccaaaagaag
     3601 gaggaggaaa aaaaaattat gataggtacc acaatgaatt atttaacaat aaaataaatc
     3661 aaaataaggt acacatcgat aataatgaat tagtaaatta ttttgatgaa ttgtatgaac
     3721 aaagtaaaaa tgaaaatatt attacaaagg gtgaggagga agaggtagag ttcaattcat
     3781 caaacgtttt gttacccttt gttgaggagc atgaacatgt aaaagataaa aatgataatg
     3841 ataaatgtat aaaatatgaa tatgttaatg ataaatgtat aaaagatgaa catggtgaat
     3901 tcgaaagagt agaagagaat ataaaattaa gtgaagatat aataaatatt attgaaaata
     3961 ttttaaaaaa atataatgtt gttttattta tgaaaggcac agctttaaat ccttattgta
     4021 aatatagtaa acaagctatt cacattttaa aattaaataa agtaaaacaa attcatacag
     4081 tcaatatttt agataatcaa gaattaagaa acgctttaaa aatttattcg aactggccta
     4141 catttcctca attatatggc ggccgccggc ttgtcgacga cggcggtctc cgtcgtcagg
     4201 atcatcgcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt gagggttaat
     4261 tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     4321 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     4381 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     4441 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     4501 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4561 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4621 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4681 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4741 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4801 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4861 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4921 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4981 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     5041 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     5101 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     5161 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     5221 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     5281 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     5341 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     5401 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     5461 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     5521 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     5581 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     5641 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     5701 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     5761 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     5821 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     5881 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     5941 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     6001 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     6061 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     6121 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     6181 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     6241 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     6301 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     6361 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     6421 aaaagtgcca c
//
