pCDS-At vector (V008580)

LOCUS       40924_10306        3919 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pCDS-At, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Kaiser J, Rohdich F.
  TITLE     Thiazolopyrimidine inhibitors of 2-methylerythritol 
            2,4-cyclodiphosphate synthase (IspF) from Mycobacterium tuberculosis
            and Plasmodium falciparum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Kaiser J, Rohdich F.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2010) Chemistry, Food Chemistry, Grindelallee 117,
            Hamburg 20146, Germany
REFERENCE   3  (bases 1 to 3919)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3919)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-FEB-2010) Chemistry, Food Chemistry, Grindelallee 117, Hamburg 
            20146, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3919
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             97..108
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             127..144
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      666..760
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             804..1460
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1528..1614
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1769..1909
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2095..2683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2857..3714)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3715..3819)
                     /label=AmpR promoter