LOCUS       40924_10306        3919 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pCDS-At, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Kaiser J, Rohdich F.
  TITLE     Thiazolopyrimidine inhibitors of 2-methylerythritol 
            2,4-cyclodiphosphate synthase (IspF) from Mycobacterium tuberculosis
            and Plasmodium falciparum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Kaiser J, Rohdich F.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2010) Chemistry, Food Chemistry, Grindelallee 117,
            Hamburg 20146, Germany
REFERENCE   3  (bases 1 to 3919)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3919)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-FEB-2010) Chemistry, Food Chemistry, Grindelallee 117, Hamburg 
            20146, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3919
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             97..108
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             127..144
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      666..760
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             804..1460
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1528..1614
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1769..1909
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2095..2683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2857..3714)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3715..3819)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
      121 ggatcgcatc accatcacca tcacggatcc cgtatcggtc acggcttcga ccttcatcgt
      181 ttggagcccg gttacccgct gatcattggt ggcattgtta tcccacatga ccgcggctgc
      241 gaagcacata gtgacggcga tgtgctgctg cattgcgtgg tggatgctat cctcggtgcc
      301 ctgggcttac cggacattgg tcagattttt cctgatagtg atcctaaatg gaaaggtgca
      361 gcgtcgtcag tgtttatcaa agaggcggtc cgcctgatgg acgaagccgg ctatgaaatc
      421 ggtaatctgg atgcgactct gattctgcaa cgtccgaaga tctcaccaca taaagagacc
      481 atccgttcta acctcagcaa gctgttaggc gccgatccgt ccgtggttaa ccttaaagcg
      541 aagacgcacg aaaaagtaga tagcctgggt gaaaatcgtt ctattgcagc tcacacagtc
      601 attctgttga tgaaaaaata ataagtcgac gtcgacctgc agccaagctt aattagctga
      661 gcttggactc ctgttgatag atccagtaat gacctcagaa ctccatctgg atttgttcag
      721 aacgctcggt tgccgccggg cgttttttat tggtgagaat ccaagctagc ttggcgagat
      781 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      841 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      901 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      961 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
     1021 ttcgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
     1081 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
     1141 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
     1201 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
     1261 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
     1321 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
     1381 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
     1441 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
     1501 gggtaatgac tctctagctt gaggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1561 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccc
     1621 tctagagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     1681 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     1741 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
     1801 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     1861 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
     1921 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     1981 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     2041 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     2101 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     2161 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     2221 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     2281 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     2341 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     2401 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     2461 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     2521 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     2581 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     2641 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     2701 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     2761 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     2821 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     2881 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     2941 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     3001 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     3061 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     3121 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     3181 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     3241 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     3301 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     3361 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     3421 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     3481 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     3541 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     3601 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     3661 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     3721 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     3781 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     3841 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     3901 gaggcccttt cgtcttcac
//
