pEKH-sigI6 vector (V007584)

LOCUS       V007584                 8923 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007584
VERSION     V007584
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8923)
  AUTHORS   Sand A, Holwerda EK, Ruppertsberger NM, Maloney M, Olson DG, Nataf
            Y, Borovok I, Sonenshein AL, Bayer EA, Lamed R, Lynd LR, Shoham Y.
  TITLE     Three cellulosomal xylanase genes in Clostridium thermocellum are
            regulated by both vegetative SigA (sigma(A)) and alternative SigI6
            (sigma(I6)) factors
  JOURNAL   FEBS Lett. 589 (20), 3133-3140 (2015)
   PUBMED   26320414
REFERENCE   2  (bases 1 to 8923)
  AUTHORS   Holwerda EK.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JUL-2015) Thayer School of Engineering, Dartmouth
            College, 14 Engineering drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 8923)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8923)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "FEBS
            Lett."; date: "2015"; volume: "589"; issue: "20"; pages: "3133-3140"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-JUL-2015) Thayer School of Engineering, Dartmouth College, 14
            Engineering drive, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(561..1418)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1419..1523)
                     /label="AmpR promoter"
     CDS             3510..4157
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             4178..4723
                     /codon_start=1
                     /product="hypoxanthine phosphoribosyltransferase"
                     /EC_number="2.4.2.8"
                     /label="hypoxanthine phosphoribosyltransferase"
                     /protein_id="ALI61576.1"
                     /translation="MENLSKDIDEILITEEELKEKIKELGRQITKDYKGKNLMLVGVLK
                     GALMFMADLSRHIDLPLSLDFMAVSSYGSSTHSSGIVKIIKDLDISIEGKDVLIVEDII
                     DSGLTLSYLRETLLGRKPKSLKICTILDKPERREASVKVDYVGFKIPDKFVVGYGLDFD
                     EKYRNLPFIGVLKPEMYS"
     terminator      5521..5709
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     rep_origin      complement(5969..6557)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6644..7222)
                     /codon_start=1
                     /product="thymidine kinase"
                     /EC_number="2.7.1.21"
                     /label="thymidine kinase"
                     /protein_id="ALI61575.1"
                     /translation="MYGPKDHGYIEVVTGPMFSGKSEELIRRIKRAKIARQKVQVFKPA
                     IDDRYSIDKVVSHNGDNMHAIAIVKASDILAYAEEDTDVFAIDEVQFFDSEIVDIVKEI
                     ADSGKRVICAGLDMDFRGEPFGPTPELMAIAEFVDKLTAICMKCGNPATRTQRLINGKP
                     ANYDDPIIMVGAKESYEARCRKCHEVPRT"
     CDS             complement(7922..8923)
                     /label="repB"
                     /note="RepB replication protein"