LOCUS       V007584                 8923 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007584
VERSION     V007584
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8923)
  AUTHORS   Sand A, Holwerda EK, Ruppertsberger NM, Maloney M, Olson DG, Nataf
            Y, Borovok I, Sonenshein AL, Bayer EA, Lamed R, Lynd LR, Shoham Y.
  TITLE     Three cellulosomal xylanase genes in Clostridium thermocellum are
            regulated by both vegetative SigA (sigma(A)) and alternative SigI6
            (sigma(I6)) factors
  JOURNAL   FEBS Lett. 589 (20), 3133-3140 (2015)
   PUBMED   26320414
REFERENCE   2  (bases 1 to 8923)
  AUTHORS   Holwerda EK.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JUL-2015) Thayer School of Engineering, Dartmouth
            College, 14 Engineering drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 8923)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8923)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "FEBS
            Lett."; date: "2015"; volume: "589"; issue: "20"; pages: "3133-3140"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-JUL-2015) Thayer School of Engineering, Dartmouth College, 14
            Engineering drive, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(561..1418)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1419..1523)
                     /label="AmpR promoter"
     CDS             3510..4157
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             4178..4723
                     /codon_start=1
                     /product="hypoxanthine phosphoribosyltransferase"
                     /EC_number="2.4.2.8"
                     /label="hypoxanthine phosphoribosyltransferase"
                     /protein_id="ALI61576.1"
                     /translation="MENLSKDIDEILITEEELKEKIKELGRQITKDYKGKNLMLVGVLK
                     GALMFMADLSRHIDLPLSLDFMAVSSYGSSTHSSGIVKIIKDLDISIEGKDVLIVEDII
                     DSGLTLSYLRETLLGRKPKSLKICTILDKPERREASVKVDYVGFKIPDKFVVGYGLDFD
                     EKYRNLPFIGVLKPEMYS"
     terminator      5521..5709
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     rep_origin      complement(5969..6557)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6644..7222)
                     /codon_start=1
                     /product="thymidine kinase"
                     /EC_number="2.7.1.21"
                     /label="thymidine kinase"
                     /protein_id="ALI61575.1"
                     /translation="MYGPKDHGYIEVVTGPMFSGKSEELIRRIKRAKIARQKVQVFKPA
                     IDDRYSIDKVVSHNGDNMHAIAIVKASDILAYAEEDTDVFAIDEVQFFDSEIVDIVKEI
                     ADSGKRVICAGLDMDFRGEPFGPTPELMAIAEFVDKLTAICMKCGNPATRTQRLINGKP
                     ANYDDPIIMVGAKESYEARCRKCHEVPRT"
     CDS             complement(7922..8923)
                     /label="repB"
                     /note="RepB replication protein"
ORIGIN
        1 ctatttagtt atttgtttgg tcacttataa ctttaacaga tggggttttt ctgtgcaacc
       61 aattttaagg gttttcaata ctttaaaaca catacatacc aacacttcaa cgcacctttc
      121 agcaactaaa ataaaaatga cgttatttct atatgtatca agaatagaaa gaactcgttt
      181 ttcgctacgc tcaaaacgca aaaaaagcac tcattcgagt gctttttctt atcgctccaa
      241 atcatgcgat tttttcctct ttgcttttct ttgctcacga agttctcgat cacgctgcaa
      301 aacatcttga agcgaaaaag tattcttctt ttcttccgat cgctcatgct gacgcacgaa
      361 aagccctcta ggcgcatagg aacaactcct aaatgcatgt gaggggtttt ctcgtccatg
      421 tgaacagtcg catacgcaat attttgtttc ccatagcggc cgcgattatc aaaaaggatc
      481 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
      541 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
      601 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
      661 cgcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
      721 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
      781 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
      841 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
      901 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
      961 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     1021 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     1081 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     1141 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     1201 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     1261 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     1321 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     1381 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     1441 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     1501 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     1561 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcg ataaaccgcc
     1621 ttgttcacat catcaaaaag ctgaccgatt ttattttgaa cgtccttatt ctccgaacct
     1681 tcccctgctt ttaaatcaca ggctgtggat ataaggatta tattctcctt ctccctggaa
     1741 ataaatccaa gctccatgct cttttgcaca atctcattca cagcctccgc cactgttttt
     1801 tcttttaatt caagtccttc cagcactgtc tcggcatcgc cgttcagtgc tttggcttcg
     1861 gctattttgt tgttgtaatt tatccaaagc tcaatacttg gatttatatc aatgccaaca
     1921 taagcatata tattttttct tgccgtattt ccattaataa acatcagcac aaaagacaac
     1981 acaattacaa agcatgcagc aactgccgca accggcaaat atctgcgcaa agaattttta
     2041 ggcttgatta tatccgagtc caaaaccttc acttttttac caactgccat ttcagaggtc
     2101 cttaccacat tcagaaaagc aaagtcatca gtcattataa tggccgtctt ttcatccata
     2161 tcaagaactt ttcctacaat caccgccggt taatttcacc ccttattctt atatcgattc
     2221 gtataccgga atggttttat gtcgcattgt cagcttccca ggtggatcaa gatatttgag
     2281 acccaatttt gcaaacattt tgcgttgttc gtgcagcgaa tcggatgtat gacccaaata
     2341 tcttttacaa ttgcaaaaac attctccaca attccccatg gtagattatt aacccaattc
     2401 ccgcagcgat gcttaaaata ttggctgtca atgcaaacag tatccgcctc agttttgaat
     2461 gttgctccag gaagtttgca tacaggtaaa cttcagctat caatattatc aattcaagaa
     2521 gcaaatacgc caaaaccgcc tccacccttc ctctactgta gagcacagca gccaaaagaa
     2581 ggctcaaaaa aagcctgggt aaatatatta attgccaaaa acggcacacc gtcgcgctcc
     2641 atgtcaaatc taaacagcaa taatacaagt ccttcaacaa tcaatgcctt caaacaggtg
     2701 caaacaaata aaatcaaaaa agccaaacct atcgaaagtt ccgttgcttc ctctgacatg
     2761 taatcaaaaa aaacaatact gtcaaacgct tttctttcta ttacgttgga aacggcggtc
     2821 atgccgtcgg aagtcaccac tatgatttta aatttgtcaa tctttctgtc aaaagtaaac
     2881 ttcttaacac atttgccgtc tttcacatcg cattcaatat cgttaagtcc catcgtggga
     2941 ataggcatgg aaaagatttt tttgcccgag gatgcattga taaagtattt cttttccgaa
     3001 agagaggaaa agattctaaa gagttttgga aatactgatg aatattgtgt gcagagtaca
     3061 attctatgga caagaaaaga ggctttgtca aaactttttc gtctgggaat gaggatggat
     3121 tttaaaaagc tggatacttt ggagggcgag gtggtttttc aggaaacaaa cagggcgcgt
     3181 ctgttttctt ttatatgcaa taattactgt atctctctgg cattgccagg ttttaataaa
     3241 gattaaaatt attgactaga aataaaaaaa ttgtccataa tattaatgga caaaaaaaca
     3301 aagaattaca tcaaaggaag ataaaaatac tttgttaaaa aattaattat tttttatcta
     3361 aactattgaa aatgaaaata aaataatata aaatgaatca tagtgcaaga gatacttgcc
     3421 agaggatgaa tattttactg cattcatgct ttatggcagc taatagaggc attaaattaa
     3481 attttaattt acaataggag gcgatattaa tgaactttaa taaaattgat ttagacaatt
     3541 ggaagagaaa agagatattt aatcattatt tgaaccaaca aacgactttt agtataacca
     3601 cagaaattga tattagtgtt ttataccgaa acataaaaca agaaggatat aaattttacc
     3661 ctgcatttat tttcttagtg acaagggtga taaactcaaa tacagctttt agaactggtt
     3721 acaatagcga cggagagtta ggttattggg ataagttaga gccactttat acaatttttg
     3781 atggtgtatc taaaacattc tctggtattt ggactcctgt aaagaatgac ttcaaagagt
     3841 tttatgattt atacctttct gatgtagaga aatataatgg ttcggggaaa ttgtttccca
     3901 aaacacctat acctgaaaat gctttttctc tttctattat tccatggact tcatttactg
     3961 ggtttaactt aaatatcaat aataatagta attaccttct acccattatt acagcaggaa
     4021 aattcattaa taaaggtaat tcaatatatt taccgctatc tttacaggta catcattctg
     4081 tttgtgatgg ttatcatgca ggattgttta tgaactctat tcaggaattg tcagataggc
     4141 ctaatgactg gcttttataa taaaggaggt cgacgtcatg gaaaatttat caaaagacat
     4201 cgatgaaatt ttgatcacag aagaagaact taaggaaaag ataaaagagc ttgggaggca
     4261 aatcacaaaa gactacaaag ggaaaaattt gatgttggta ggagttttaa aaggtgcttt
     4321 aatgtttatg gctgatttgt caagacacat agatttgcct ttatcacttg attttatggc
     4381 tgtttccagc tatggaagct caactcattc atcaggaata gtaaagataa tcaaagatct
     4441 tgatataagc atagaaggca aagatgttct gattgtggaa gacataattg acagcggttt
     4501 gactttgtct tacttaaggg aaactttact tggaaggaag ccaaaaagcc tgaaaatatg
     4561 cacaatatta gacaaaccgg agagaagaga agcatctgta aaagtcgatt atgtaggatt
     4621 taagatacct gataagtttg tcgtgggtta tggattggac tttgatgaaa agtacaggaa
     4681 ccttcctttt ataggcgttt tgaaacctga aatgtacagc taagcccggg cctcgagaaa
     4741 acaaaaggct cagtcggaag actgggcctt ttgttttggt accgaattcg gcgcgcctca
     4801 gcgtttaaac ccgctgatcc tagagggccg catcatgtaa ttagttatgt cacgcttaca
     4861 ttcacgccct ccccatgctt taatacatac ctggcccagt agttcagtgg gcaacttttt
     4921 ggtttttttt agcttttcat aaagcccgtc attacttgca attttttttg caatatttat
     4981 caaaagctct ctcgaatctc tgtgctttgg agtacaggag agcaaatcct taaaagttat
     5041 atcaaaggag gccagctcag atttgaaaag tctaatctcg tccgtaaatt ccaatctttc
     5101 aattgcattg ttgccgtcag catccgaaag agttctttca agtttgatat cttcgttttc
     5161 aaagtaagaa aaaggataaa ccattttatt cttatgattt tttcttttat agtcaataag
     5221 tcttctatta ataacctgtt ctgagaaaac aaggaagtta gaatgcttct cttcatcata
     5281 agcattgatg gcttcattga aagccaataa tgcaacgctg tattcttcac tgttttccgg
     5341 ctcaacatgc ctgtcagtcg ccttatacac caattttaat ataaaaggcc taaacctcag
     5401 gataaactct tcccttgcag aatcatcccc ggcttttatt ctgttcagat actctataat
     5461 aatccttttt gtatgttccc tgtcgtcgct gctaatagta gtgaaaaaat cagggataat
     5521 gccgctctaa ccgaaaagga aggagttaga caacctgaag tctaggtccc tatttatttt
     5581 tttatagtta tgttagtatt aagaacgtta tttatatttc aaatttttct tttttttctg
     5641 tacagacgcg tgtacgcatg taacattata ctgaaaacct tgcttgagaa ggttttggga
     5701 cgctcgaagg ctttaatttg caagctgcgg ccctgcatta atgaatcggc caacgcgcgg
     5761 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     5821 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     5881 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     5941 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6001 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6061 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     6121 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     6181 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     6241 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     6301 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     6361 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     6421 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     6481 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     6541 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     6601 acgaaaactc acgttaaggg attttggtca tgaaaaaatc cgcttaagtc cgcgggactt
     6661 catggcactt tctacacctt gcttcataag actcctttgc tccaaccatt ataatgggat
     6721 cgtcgtaatt ggcaggcttc ccatttataa gcctttgcgt gcgagtagca ggattgccac
     6781 acttcatgca tatagcagta agcttatcga caaattcagc tatggccatc aattctggag
     6841 tcggaccaaa tggttcacct ctaaagtcca tgtcaagccc tgcacatatg actcttttcc
     6901 cgctatcggc aatctctttt acgatgtcga ctatttcaga atcaaaaaat tgaacttcat
     6961 ctatagcaaa tacatccgta tcttcttcag cataagccaa tatgtcagaa gcctttacta
     7021 tggcaatggc gtgcatgttg tcgccgttat gagatacgac tttgtctatg gaatacctat
     7081 cgtctatagc cggtttgaaa acctgaactt tttgcctggc aatcttagct ctctttatcc
     7141 ttcttataag ttcctcactt tttccactga acatgggacc agttacaact tctatgtacc
     7201 cgtggtcttt aggcccatac atctttcgtc ctccttaaaa ttttcgttta tttgtttttt
     7261 atttagtgtg cctaaaagca ccatcttata cattcgtacc ttatatctct tatcttgtat
     7321 ctcttacctc atatctctta ttattaacta ccgaagtata ttttcataac ggaatcttat
     7381 gacttatttt tctgactgcc gttttaccat acggttaatt tatacggaaa cagctaatct
     7441 aaccttgctg aaggcatgtt taccaaaata ttatatctac tttaccatat taattcaagt
     7501 ggtcattttg atattttacg ccaaaatttt tagcgttcct ttacttgata atttatccaa
     7561 aaaccttcat tcgtcaaaac aattatgata taattaattt atgatatgat taattaatat
     7621 ttacggataa ctaatcattt caatgcaata gatcaacatg caaatcaata tccaccttac
     7681 tccgaacatt ttgcaccaac gaatgcccag cactatatcc cggcaagcaa tataacgtct
     7741 ataatttttt atgataacga taaaattaaa ttttatgaaa ggggtaaaag catgagaaaa
     7801 atagctgtat tgttaattac tttgacgttc ttagtcacga ctcgaattta ggaggcttac
     7861 ttgtctgctt tcttcattag aatcaatcct tttttaaaag tcaatcccgt ttgttgaact
     7921 actctttaat aaaataattt ttccgttccc aattccacat tgcaataata gaaaatccat
     7981 cttcatcggc tttttcgtca tcatctgtat gaatcaaatc gccttcttct gtgtcatcaa
     8041 ggtttaattt tttatgtatt tcttttaaca aaccaccata ggagattaac cttttacggt
     8101 gtaaaccttc ctccaaatca gacaaacgtt tcaaattctt ttcttcatca tcggtcataa
     8161 aatccgtatc ctttacagga tattttgcag tttcgtcaat tgccgattgt atatccgatt
     8221 tatatttatt tttcggtcga atcatttgaa cttttacatt tggatcatag tctaatttca
     8281 ttgccttttt ccaaaattga atccattgtt tttgattcac gtagttttct gtattcttaa
     8341 aataagttgg ttccacacat accaatacat gcatgtgctg attataagaa ttatctttat
     8401 tatttattgt cacttccgtt gcacgcataa aaccaacaag atttttatta atttttttat
     8461 attgcatcat tcggcgaaat ccttgagcca tatctgacaa actcttattt aattcttcgc
     8521 catcataaac atttttaact gttaatgtga gaaacaacca acgaactgtt ggcttttgtt
     8581 taataacttc agcaacaacc ttttgtgact gaatgccatg tttcattgct ctcctccagt
     8641 tgcacattgg acaaagcctg gatttacaaa accacactcg atacaacttt ctttcgcctg
     8701 tttcacgatt ttgtttatac tctaatattt cagcacaatc ttttactctt tcagcctttt
     8761 taaattcaag aatatgcaga agttcaaagt aatcaacatt agcgattttc ttttctctcc
     8821 atggtctcac ttttccactt tttgtcttgt ccactaaaac ccttgatttt tcatctgaat
     8881 aaatgctact attaggacac ataatattaa aagaaacccc cat
//
