Basic Vector Information
- Vector Name:
- pEGFP-Pax9
- Antibiotic Resistance:
- Kanamycin
- Length:
- 5738 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Wang Z, Lan J, Chen Z, Fan M.
pEGFP-Pax9 vector Map
pEGFP-Pax9 vector Sequence
LOCUS V007591 5738 bp DNA circular SYN 17-DEC-2018
DEFINITION Exported.
ACCESSION V007591
VERSION V007591
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 5738)
AUTHORS Wang Z, Lan J, Chen Z, Fan M.
TITLE The Construction and Expression of Green Fluorescent Protein (GFP)-
Paired-box Gene 9 (Pax9) Fused Protein Eukaryotic Cell Expression
Plasmid and Subcellular Location of Pax9
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 5738)
AUTHORS Wang Z, Lan J, Chen Z, Fan M.
TITLE Direct Submission
JOURNAL Submitted (29-APR-2005) School of Stomatology, Wuhan University,
Luoyu Road 65#, Wuhan, Hubei 430079, China
REFERENCE 3 (bases 1 to 5738)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 5738)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(29-APR-2005) School of Stomatology, Wuhan University, Luoyu Road
65#, Wuhan, Hubei 430079, China"
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5738
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 61..364
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 613..1329
/label="EGFP"
/note="enhanced GFP"
CDS 1366..2391
/gene="Pax9"
/label="Paired box protein Pax-9"
/note="Paired box protein Pax-9 from Mus musculus.
Accession#: P47242"
polyA_signal 2526..2647
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(2654..3109)
/direction=LEFT
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3136..3240
/label="AmpR promoter"
promoter 3242..3599
/label="SV40 promoter"
/note="SV40 enhancer and early promoter"
CDS 3634..4425
/label="NeoR/KanR"
/note="aminoglycoside phosphotransferase"
polyA_signal 4660..4707
/label="HSV TK poly(A) signal"
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5036..5624
/direction=RIGHT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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