LOCUS       V007591                 5738 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007591
VERSION     V007591
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Wang Z, Lan J, Chen Z, Fan M.
  TITLE     The Construction and Expression of Green Fluorescent Protein (GFP)-
            Paired-box Gene 9 (Pax9) Fused Protein Eukaryotic Cell Expression
            Plasmid and Subcellular Location of Pax9
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5738)
  AUTHORS   Wang Z, Lan J, Chen Z, Fan M.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2005) School of Stomatology, Wuhan University,
            Luoyu Road 65#, Wuhan, Hubei 430079, China
REFERENCE   3  (bases 1 to 5738)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5738)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (29-APR-2005) School of Stomatology, Wuhan University, Luoyu Road
            65#, Wuhan, Hubei 430079, China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             613..1329
                     /label="EGFP"
                     /note="enhanced GFP"
     CDS             1366..2391
                     /gene="Pax9"
                     /label="Paired box protein Pax-9"
                     /note="Paired box protein Pax-9 from Mus musculus.
                     Accession#: P47242"
     polyA_signal    2526..2647
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2654..3109)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3136..3240
                     /label="AmpR promoter"
     promoter        3242..3599
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3634..4425
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4660..4707
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5036..5624
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attccatgga gccagccttc
     1381 ggggaggtga accagctggg aggagtgttc gtgaacggaa ggccgctgcc caacgccatt
     1441 cggcttcgca tcgtggaatt agcccaactg ggcatccgac cttgtgacat cagccgccag
     1501 ctacgggtct cgcacggctg cgtcagcaag atcctggcgc gctacaacga gaccggttcg
     1561 attttgccgg gagctattgg ggggagcaag ccccgggtca ccacccctac tgtggtgaaa
     1621 cacatccgga cttacaagca gagggaccca ggcatcttcg cttgggagat ccgggaccgc
     1681 ctgctggcgg atggcgtgtg cgacaagtac aacgtgccct cggtgagttc catcagccgt
     1741 attctgcgca acaagatcgg caacttggcc cagcagggtc attacgactc atacaagcag
     1801 caccagcccg cgccgcagcc cgcgctgccc tacaaccaca tttactcata tcccagtccc
     1861 atcacggcgg cagcagctaa ggtgcctaca ccacctgggg tgccggccat ccccggatcg
     1921 gtggccttgc cgcgcacctg gccctcctct cactccgtca cggacattct gggcatccgc
     1981 tccatcaccg accaaggagt gagcgacagc tccccctacc acagccccaa ggtggaggag
     2041 tggagcagcc tgggccgcaa caacttccct gccgccgccc cgcacgcagt gaatggattg
     2101 gagaagggag ccttggagca ggaagccaag tacggccagg caccgaatgg tctcccagct
     2161 gtgagcagtt tcgtctcagc atccagcatg gctccttacc ctacaccagc ccaagtgtca
     2221 ccctacatga cctacagtgc tgctccttct ggttatgtgg ctggacatgg gtggcaacat
     2281 gccggcagca ccccactgtc cccccacaac tgtgacattc ccgcatcgct ggctttcaag
     2341 ggaatgcagg cagccaggga aggcagccat tctgtgaccg cttctgcact ctgacgggat
     2401 ccaccggatc tagataactg atcataatca gccataccac atttgtagag gttttacttg
     2461 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     2521 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     2581 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     2641 tatcttaacg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     2701 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     2761 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     2821 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     2881 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     2941 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     3001 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     3061 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact
     3121 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3181 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     3241 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     3301 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     3361 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3421 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     3481 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     3541 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag
     3601 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     3661 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     3721 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     3781 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
     3841 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     3901 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     3961 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     4021 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     4081 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     4141 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
     4201 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     4261 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     4321 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     4381 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     4441 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     4501 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     4561 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa
     4621 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
     4681 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
     4741 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
     4801 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
     4861 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt
     4921 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     4981 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5041 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5101 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5161 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     5221 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5281 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     5341 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     5401 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     5461 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     5521 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     5581 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     5641 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     5701 cccctgattc tgtggataac cgtattaccg ccatgcat
//
