Basic Vector Information
- Vector Name:
- pEGFP-hnRNPC1
- Antibiotic Resistance:
- Kanamycin
- Length:
- 5631 bp
- Type:
- Mammalian expression vector
- Replication origin:
- ori
- Source/Author:
- De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R.
- Promoter:
- CMV
pEGFP-hnRNPC1 vector Map
pEGFP-hnRNPC1 vector Sequence
LOCUS V007599 5631 bp DNA circular SYN 17-DEC-2018
DEFINITION Exported.
ACCESSION V007599
VERSION V007599
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 5631)
AUTHORS De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
Fonseca M, Vanhoucke M, Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 5631)
AUTHORS De Schamphelaire W.
TITLE Direct Submission
JOURNAL Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM
REFERENCE 3 (bases 1 to 5631)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 5631)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
9052, BELGIUM"
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5631
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 61..364
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 613..1329
/label="EGFP"
/note="enhanced GFP"
CDS 1381..2286
/gene="HNRNPC"
/label="Heterogeneous nuclear ribonucleoprotein C"
/note="Heterogeneous nuclear ribonucleoprotein C from Pongo
abelii. Accession#: Q5RA82"
polyA_signal 2419..2540
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(2547..3002)
/direction=LEFT
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3029..3133
/label="AmpR promoter"
promoter 3135..3492
/label="SV40 promoter"
/note="SV40 enhancer and early promoter"
CDS 3527..4318
/label="NeoR/KanR"
/note="aminoglycoside phosphotransferase"
polyA_signal 4553..4600
/label="HSV TK poly(A) signal"
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4929..5517
/direction=RIGHT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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