LOCUS       V007599                 5631 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007599
VERSION     V007599
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5631)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5631)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5631
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             613..1329
                     /label="EGFP"
                     /note="enhanced GFP"
     CDS             1381..2286
                     /gene="HNRNPC"
                     /label="Heterogeneous nuclear ribonucleoprotein C"
                     /note="Heterogeneous nuclear ribonucleoprotein C from Pongo
                     abelii. Accession#: Q5RA82"
     polyA_signal    2419..2540
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2547..3002)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3029..3133
                     /label="AmpR promoter"
     promoter        3135..3492
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3527..4318
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4553..4600
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4929..5517
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc
     1381 atggccagca acgttaccaa caagacagat cctcgctcca tgaactcccg tgtattcatt
     1441 gggaatctca acactcttgt ggtcaagaaa tctgatgtgg aggcaatctt ttcgaagtat
     1501 ggcaaaattg tgggctgctc tgttcataag ggctttgcct tcgttcagta tgttaatgag
     1561 agaaatgccc gggctgctgt agcaggagag gatggcagaa tgattgctgg ccaggtttta
     1621 gatattaacc tggctgcaga gccaaaagtg aaccgaggaa aagcaggtgt gaaacgatct
     1681 gcagcggaga tgtacgggtc agtaacagaa cacccttctc cgtcccctct actcagctcc
     1741 tcttttgact tggactatga ctttcaacgg gactattatg ataggatgta cagttaccca
     1801 gcacgtgtac ctcctcctcc tcctattgct cgggctgtag tgccctcgaa acgtcagcgt
     1861 gtatcaggaa acacttcacg aaggggcaaa agtggcttca attctaagag tggacagcgg
     1921 ggatcttcca agtctggaaa gttgaaagga gatgaccttc aggccattaa gaaggagctg
     1981 acccagataa aacaaaaagt ggattctctc ctggaaaacc tggaaaaaat ggaaaaggaa
     2041 cagagcaaac aagcagtaga gatgaagaat gataagtcag aagaggagca gagcagcagc
     2101 tccgtgaaga aagatgagac taatgtgaag atggagtctg aggggggtgc agatgactct
     2161 gctgaggagg gggacctact ggatgatgat gataatgaag atcgggggga tgaccagctg
     2221 gagttgatca aggatgatga aaaagaggct gaggaaggag aggatgacag agacagcgcc
     2281 aatggaggag gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta
     2341 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     2401 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     2461 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     2521 aaactcatca atgtatctta acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc
     2581 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     2641 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     2701 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     2761 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     2821 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     2881 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     2941 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     3001 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     3061 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     3121 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     3181 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     3241 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     3301 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     3361 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     3421 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     3481 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     3541 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     3601 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     3661 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
     3721 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     3781 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     3841 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     3901 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     3961 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     4021 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
     4081 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     4141 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     4201 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     4261 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     4321 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     4381 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     4441 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg
     4501 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
     4561 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
     4621 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
     4681 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
     4741 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta
     4801 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     4861 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     4921 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     4981 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     5041 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     5101 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     5161 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     5221 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     5281 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     5341 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     5401 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     5461 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     5521 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     5581 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca t
//
