pEF-BOShFasRd vector (V007641)

LOCUS       V007641                 6412 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007641
VERSION     V007641
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6412)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6412)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6412)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6412)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      207..587
                     /label="M13 ori"
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     855..871
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      complement(918..1484)
                     /label="polyA"
                     /note="polyA"
                     /regulatory_class="terminator"
     CDS             complement(1490..2449)
                     /gene="FAS"
                     /label="Tumor necrosis factor receptor superfamily member
                     6"
                     /note="Tumor necrosis factor receptor superfamily member 6
                     from Homo sapiens. Accession#: P25445"
     promoter        complement(2677..3855)
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             complement(3861..3930)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL87972.1"
                     /translation="MDKVLNREESLQLMDLLGLERSA"
     promoter        complement(3952..4162)
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     primer_bind     complement(4191..4207)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4215..4231)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4239..4269)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4284..4305)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4593..5181)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5355..6212)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6213..6317)
                     /label="AmpR promoter"