LOCUS       V007641                 6412 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007641
VERSION     V007641
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6412)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6412)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6412)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6412)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      207..587
                     /label="M13 ori"
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     855..871
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      complement(918..1484)
                     /label="polyA"
                     /note="polyA"
                     /regulatory_class="terminator"
     CDS             complement(1490..2449)
                     /gene="FAS"
                     /label="Tumor necrosis factor receptor superfamily member
                     6"
                     /note="Tumor necrosis factor receptor superfamily member 6
                     from Homo sapiens. Accession#: P25445"
     promoter        complement(2677..3855)
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             complement(3861..3930)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL87972.1"
                     /translation="MDKVLNREESLQLMDLLGLERSA"
     promoter        complement(3952..4162)
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     primer_bind     complement(4191..4207)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4215..4231)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4239..4269)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4284..4305)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4593..5181)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5355..6212)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6213..6317)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatgctcg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
      241 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
      301 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
      361 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
      421 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
      481 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
      541 tatctcgggc tattcttttg atttataagg gattttgccg atttcggaac caccatcaaa
      601 caggattttc gcctgctggg gcaaaccagc gtggaccgaa ttttaacaaa atattaacgt
      661 tttgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
      721 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      781 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      841 ccagtcacga cgttgtaaaa cgacggccag tgaattcttt tttttttttt tttttttttt
      901 tttttttttt tttttttgga ggcaaacact ttattatcct gaagttcagt tacaatgtga
      961 cagtggaggg gacactggtg agtggggggt gaagaggtgg agggtgacag tggagcttcc
     1021 tttcacacac aggcctgaca tgattcctcc tgtctgctcc ctcccacatc cccagtcccc
     1081 atccagcaag gcaaatgtcc aggcacctgc ctggccctaa aaagagtcac agtcctgacc
     1141 ctcgtggggg cagggcaggt gtcccgaggc cacccagaaa aacaggagac acgtcggtga
     1201 tgttcgggag tcaaaccatg tcccaaaagt cttaagaaga aaaacgagat tccccagcaa
     1261 attcgtggga ccccagggcc atgcctccca gctctggcca cctctgcttg tccaggggcc
     1321 tcacagccaa ggggatgcag gggccgctgt gcagtcggct gaggccagag tgaggggtgc
     1381 aaggacccag atggggaaca ctgctgttta aatattaaac agggatttct tgtctcccac
     1441 gtgggagacc tgatactctc aagggtccca ggtggggacc ctcactctag ctagtaatgt
     1501 ccttgaggat gatagtctga attttctctg caagagtaca aagattggct tttttgagat
     1561 ctttaatcaa tgtgtcatac gcttctttct ttccatgaag ttgatgccaa ttacgaagca
     1621 gttgaacttt ctgttctgct gtgtcttgga cattgtcatt cttgatctca tctattttgg
     1681 cttcattgac accattcttt cgaacaaagc ctttaacttg acttagtgtc atgactccag
     1741 caatagtggt gatatattta ctcaagtcaa catcagataa atttattgcc actgtttcag
     1801 gatttaaggt tggagattca tgagaacctt ggttttcctt tctgtgcttt ctgcatgttt
     1861 tctgtacttc ctttctcttc acccaaacaa ttagtggaat tggcaaaaga agaagacaaa
     1921 gccaccccaa gttagatctg gatccttcct ctttgcactt ggtgttgctg gtgagtgtgc
     1981 attccttgat gattccatgt tcacatttgg tgcaagggtc acagtgttca catacagtag
     2041 agttacaaaa aaagtttggt ttacatctgc acttggtatt ctgggtccgg gtgcagttta
     2101 tttccacttc taagccatgt ccttcatcac acaatctaca tcttctgcat ttggaagaaa
     2161 aatgggcttt gtctgtgtac tccttccctt cttggcaggg cacgcagtct ggttcatccc
     2221 cattgactgt gcagtcccta gctttccttt cacctggagg acagggctta tggcagaatt
     2281 ggccatcatg atgcaggcct tccaagttct gagtctcaac tgtagtaaca gtcttcctca
     2341 attccaatcc cttggagttg atgtcagtca cttgggcatt aacacttttg gacgataatc
     2401 tagcaacaga cgtaagaacc agaggtagga gggtccagat gcccagcatg gttgttgagc
     2461 aatcctccga agtgaaagag cttccccaac tccgtactga gcgggtccac caacccgcgg
     2521 gagaagaggc agctccgcct gggcagccag ggctggcctc agggtgtgtt ccgtgccagt
     2581 gcccgcccct gaggctccag ccaagtcact cgtaaaccgc ttccctcact ccccagaagc
     2641 gtcnnnctcg aggtcgaggg atctctagag aattcctcac gacacctgaa atggaagaaa
     2701 aaaactttga accactgtct gaggcttgag aatgaaccaa gatccaaact caaaaagggc
     2761 aaattccaag gagaattaca tcaagtgcca agctggccta acttcagtct ccacccactc
     2821 agtgtgggga aactccatcg cataaaaccc ctccccccaa cctaaagacg acgtactcca
     2881 aaagctcgag aactaatcga ggtgcctgga cggcgcccgg tactccgtgg agtcacatga
     2941 agcgacggct gaggacggaa aggccctttt cctttgtgtg ggtgactcac ccgcccgctc
     3001 tcccgagcgc cgcgtcctcc attttgagct ccctgcagca gggccgggaa gcggccatct
     3061 ttccgctcac gcaactggtg ccgaccgggc cagccttgcc gcccagggcg gggcgataca
     3121 cggcggcgcg aggccaggca ccagagcagg ccggccagct tgagactacc cccgtccgat
     3181 tctcggtggc cgcgctcgca ggccccgcct cgccgaacat gtgcgctggg acgcacgggc
     3241 cccgtcgccg cccgcggccc caaaaaccga aataccagtg tgcagatctt ggcccgcatt
     3301 tacaagacta tcttgccaga aaaaaagcgt cgcagcaggt catcaaaaat tttaaatggc
     3361 tagagactta tcgaaagcag cgagacaggc gcgaaggtgc caccagattc gcacgcggcg
     3421 gccccagcgc ccaggccagg cctcaactca agcacgaggc gaaggggctc cttaagcgca
     3481 aggcctcgaa ctctcccacc cacttccaac ccgaagctcg ggatcaagaa tcacgtactg
     3541 cagccaggtg gaagtaattc aaggcacgca agggccataa cccgtaaaga ggccaggccc
     3601 gcgggaacca cacacggcac ttacctgtgt tctggcggca aacccgttgc gaaaaagaac
     3661 gttcacggcg actactgcac ttatatacgg ttctccccca ccctcgggaa aaaggcggag
     3721 ccagtacacg acatcacttt cccagtttac cccgcgccac cttctctagg caccggttca
     3781 attgccgacc cctcccccca acttctcggg gactgtgggc gatgtgcgct ctgcccactg
     3841 acgggcaccg gagcctcacg aggcactcct ttcaagacct agaaggtcca ttagctgcaa
     3901 agattcctct ctgtttaaaa ctttatccat ctttgcaaag ctttttgcaa aagcctaggc
     3961 ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct
     4021 ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag
     4081 ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca
     4141 tgctttgcat acttctgcct gctggggagc ctaagcttgg cgtaatcatg gtcatagctg
     4201 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     4261 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     4321 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     4381 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     4441 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     4501 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     4561 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     4621 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     4681 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     4741 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     4801 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     4861 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     4921 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     4981 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     5041 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     5101 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     5161 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     5221 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     5281 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     5341 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     5401 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     5461 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     5521 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     5581 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     5641 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     5701 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     5761 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     5821 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     5881 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     5941 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     6001 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     6061 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     6121 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     6181 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     6241 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     6301 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     6361 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//
