pEAVrMLVB vector (V007691)

LOCUS       V007691                14553 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007691
VERSION     V007691
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 14553)
  AUTHORS   Zhang J, Go YY, Huang CM, Meade BJ, Lu Z, Snijder EJ, Timoney PJ,
            Balasuriya UB.
  TITLE     Development and characterization of an infectious cDNA clone of the
            modified live virus vaccine strain of equine arteritis virus
  JOURNAL   Clin. Vaccine Immunol. 19 (8), 1312-1321 (2012)
   PUBMED   22739697
REFERENCE   2  (bases 1 to 14553)
  AUTHORS   Zhang J, Balasuriya UBR.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2009) Veterinary Science, University of Kentucky,
            108 Gluck Equine Research Center, Lexington, KY 40546, USA
REFERENCE   3  (bases 1 to 14553)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14553)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Clin.
            Vaccine Immunol."; date: "2012"; volume: "19"; issue: "8"; pages:
            "1312-1321"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-MAR-2009) Veterinary Science, University of Kentucky, 108 Gluck
            Equine Research Center, Lexington, KY 40546, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             225..9751
                     /codon_start=1
                     /ribosomal_slippage=""
                     /gene="ORF1ab"
                     /product="replicase polyprotein 1ab"
                     /label="ORF1ab"
                     /protein_id="ACY79514.1"
                     /translation="MATFSATGFGGSFVRDWSLDLPDACEHGAGLCCEVDGSTLCAECF
                     RGCEGVEQCPGLFMGLLKLASPVPVGHKFLIGWYRAAKVTGRYNFLELLQHPAFAQLRV
                     VDARLAIEEASVFISTDHASAKRFPGARFALTPVYANAWVVSPAANSLIVTTDQEQDGF
                     CWLKLLPPDRREAGLRLYYNHYREQRTGWLSKTGLRLWLGDLGLGINASSGGLKFHIMR
                     GSPQRAWHITTRSCKLKSYYVCDISEADWSCLPAGNYGGYNPPGDGACGYRCLAFMNGA
                     TVVSAGCSSDLWCDDELAYRVFQLSPTFTVTIPGGRVCPNAKYAMICDKQHWRVKRAKG
                     VGLCLDESCFRGTCNCQRMSGPPPAPVSAAVLDHILEAATFGNVRVVIPEGQPRPVPAP
                     RVHPCANSSGDVKDPAPVPPVPKPRTKLAKPNPTQAPIPAPRTRLQGASTQEPLASAGV
                     ASDSAPKWRVAKTVYSSAERFRTELVQRARSVGDVLVQALPHKTPAVQRYTMTLKMMRS
                     RFSWHCDMWYPLAVIACLLPIWPSLALLLSFAIGLIPSVGNNVVLTALLVSSANYVASM
                     DHHCEGAACLALLEEEHYYRAVRWRPITGALSLVLNLLGQVGYVARSTFDAAYVPCTVF
                     DLCSFAILYLCCNRCWRCFGRCVRVGPATHVLGSTGQRVSKLALIDLCDHFSKPTIDVV
                     GMATGWSGCYTGTAAMERQCASTVDPHSFDQKKAGAIVYLTPPVNSGSALQCLNVMWKR
                     PIGSTVLGEQTGAVVTAVKSISFSPPCCVSTTLPTRPGVTVVDHALYNRLTASGVDPAL
                     LRVGQGDFLKLNPGFRLIGGWIYGICYFVLVVVSTFTCLPIKCGIGTRDPFCRRVFSVP
                     VIKTQEHCHAGMCASAEGISLDSLGLTQLQSYWIAAVTSGLVILLVCHRLAISALDLLT
                     LASPLVLLVFPWASVGLLLACSLAGAAVKIQLLATLFVNLFFPQATLVTMGYWACVAAL
                     AVYSLMGLRVKVNVPMCVTPAHFLLLARSAGQSREQMLRVSAAAPTNSLLGVARDCYVT
                     GTTRLYIPKEGGMVFEGLFRSPKARGNVGFVAGSSYGTGSVWTRNNEVVVLTASHVVGR
                     ANMATLKIGDAMLTLTFKKNGDFAEAVTTQSELPGNWPQLHFAQPTTGPASWCTATGDE
                     EGLLSGEVCLAWTTSGDSGSAVVQGDAVVGVHTGSNTSGVAYVTTPSGKLLGADTVTLS
                     SLSKHFTGPLTSIPKDIPDNIIADVDAVPRSLAMLIDGLSNRESSLSGPQLLLIACFMW
                     SYLNQPAYLPYVLGFFAANFFLPKSVGRPVVTGLLWLCCLFTPLSMRLCLFHLVCATVT
                     GNVISLWFYITAAGTSYLSEMWFGGYPTLLFVPRFLVYQFPGWAIGTVLAVCSITMLAA
                     ALGHTLLLDVFSASGRFDRTFMMKYFLEGGVKESVTASVTRAYGKPITQESLTATLAAL
                     TDDDFQFLSDVLDCRAVRSAMNLRAALTSFQVAQYRNILNASLQVDRDAARSRRLMAKL
                     ADFAVEQEVTAGDRVVVIDGLDRMAHFKDDLVLVPLTTKVVGGSRCTICDVVKEEANDT
                     PVKPMPSRRRRKGLPKGAQLEWDRHQEEKRNAGDDDFAVSTDYVKRVPKYWDPSDTRGT
                     TVKIAGTTYQKVVDYSGNVHYVEHQEDLLDYVLGKGSYEGLDQDKVLDLTNMLKVDPTE
                     LSSKDKAKARQLAHLLLDLANPVEAVNQLN*ERPTSFPAMWGVGHLLTLKTRVSWLYTV
                     AQCF*LPGTFYLTSNLCATKSSQRPQKTHCLGTYAPALVTGLFSVVRTGH*LMHTGTVW
                     SAFTRFPPYLILM*AQVTSQ*RVSDGILNLPEEAVQNVSQLIWCTLFKGSTEPLIPMMT
                     RWQLLSVVTRIGRTASCITPVGATFHILSQPMLWKPQLATVLDVRPLPTGPTSSQQLGP
                     SRSNNPYRTSQRACLTTALTSAVTLS*RPLQRQPCVVI*RNTTYPRRVLCCLVFSPWCG
                     RT*KRRLETLHHSTCHLLYHLKIHKPELTALSFLQGLYRATV*LMTWCHSP*KAIYKPP
                     PWRLVNGSTVPNTRLGAFWAPTITLA*VCVPAFRGLRPHSKKLERMGHRFIWASQNSTR
                     YLLLTSTALKQTWRVVIAPPRLWCVGSLLILFLS*LASPSWCTATC*IAVTI*LWQVV*
                     HSPNAGVCHLETLSLPFPIPSIHWCCTPSTCCYVDLKAISQRLQKNILMAAWSCGTCSS
                     TFECTSTRTMWF*PHPTSITRPALTAGSPTCRRC*VSRLTQRKL*TPAPLPFWAAGSSK
                     WTASVIWPVFRTALHALCYTTLVQRIPQSTMKLLFPSLRTPLSAVMKIGGRTSIDVSVA
                     LRVLTELSSPPLKC*HPSAPSSMRVPCAQFVGPPPWPSLLVEGGSVAIVSRTTWVIVTQ
                     PRSSPTAGTTSCTAPLTAQCVRVPQNRWYQKCLTRSWIICCATLITAVKRN*LW*WRMV
                     EQHHRPGATKWVTR*SPWLQTWEATLCLGAVLDHTSQYHFRIRSRAWW*IKL*RTPPPL
                     STWKDPLGVGRLFTWSKMC*PWSVARPWLCPPTRPCWTALTSSNKRAPIHTLWCPSIQF
                     LTFPGLAVETSQCDCHRSELVREKPLWMRWLTSHQWIWRAF*PRVESRVTVI*ISSGAS
                     DPRACHVTFGSDILSAWSPCECAIDSALLCVI*SRAFILIMSQLHIPLKWCLCQIQTLR
                     KV*SSPPTTKIAVLVTAQLIQFKAVHSLL*LFDCPHPNH*RARAQLWRLLGRLRNYTST
                     TPLISLAGC*SSPRKQRRRT*STAHLQHATWAKKLTFGPMRASNITRKSTCCTHTSPSR
                     MV*YTVTLIVALPVAGKSNPTKFRASREWHKIWATTIPQIYQDFAPYRKNSLSIGP*CP
                     MIDTRIACKLPYSKYVNSVNRAQRAIWLDNLFSCRRLV*HLTGLLNGSTAKRVLYQIPY
                     SRPVGSRLTAAFSSMKPRKSLPPLTLMPVWEKLISPPWEDPTSSFPNIYHHCYPQALLP
                     W*VLHWLGKLLKLLAALLMSMLHRLNLIYTLRH*VACTRL*SISSRVGLWCGETRPFMS
                     KRVLMQLHQH*QLCPNSSKCRPMSLFHSMWHQGTEPTRW*RPRLKFQLEPTPPSGHCQL
                     NRHRLVMRSCGDIL*RGSSAQQKCSCAAGVLCLPPQCRPFVH*RDVNLCSTSYKLVQSL
                     GPC"
     gene            225..9751
                     /gene="ORF1ab"
                     /label="ORF1ab"
     CDS             225..5408
                     /codon_start=1
                     /gene="ORF1a"
                     /product="replicase polyprotein 1a"
                     /label="ORF1a"
                     /protein_id="ACY79515.1"
                     /translation="MATFSATGFGGSFVRDWSLDLPDACEHGAGLCCEVDGSTLCAECF
                     RGCEGVEQCPGLFMGLLKLASPVPVGHKFLIGWYRAAKVTGRYNFLELLQHPAFAQLRV
                     VDARLAIEEASVFISTDHASAKRFPGARFALTPVYANAWVVSPAANSLIVTTDQEQDGF
                     CWLKLLPPDRREAGLRLYYNHYREQRTGWLSKTGLRLWLGDLGLGINASSGGLKFHIMR
                     GSPQRAWHITTRSCKLKSYYVCDISEADWSCLPAGNYGGYNPPGDGACGYRCLAFMNGA
                     TVVSAGCSSDLWCDDELAYRVFQLSPTFTVTIPGGRVCPNAKYAMICDKQHWRVKRAKG
                     VGLCLDESCFRGTCNCQRMSGPPPAPVSAAVLDHILEAATFGNVRVVIPEGQPRPVPAP
                     RVHPCANSSGDVKDPAPVPPVPKPRTKLAKPNPTQAPIPAPRTRLQGASTQEPLASAGV
                     ASDSAPKWRVAKTVYSSAERFRTELVQRARSVGDVLVQALPHKTPAVQRYTMTLKMMRS
                     RFSWHCDMWYPLAVIACLLPIWPSLALLLSFAIGLIPSVGNNVVLTALLVSSANYVASM
                     DHHCEGAACLALLEEEHYYRAVRWRPITGALSLVLNLLGQVGYVARSTFDAAYVPCTVF
                     DLCSFAILYLCCNRCWRCFGRCVRVGPATHVLGSTGQRVSKLALIDLCDHFSKPTIDVV
                     GMATGWSGCYTGTAAMERQCASTVDPHSFDQKKAGAIVYLTPPVNSGSALQCLNVMWKR
                     PIGSTVLGEQTGAVVTAVKSISFSPPCCVSTTLPTRPGVTVVDHALYNRLTASGVDPAL
                     LRVGQGDFLKLNPGFRLIGGWIYGICYFVLVVVSTFTCLPIKCGIGTRDPFCRRVFSVP
                     VIKTQEHCHAGMCASAEGISLDSLGLTQLQSYWIAAVTSGLVILLVCHRLAISALDLLT
                     LASPLVLLVFPWASVGLLLACSLAGAAVKIQLLATLFVNLFFPQATLVTMGYWACVAAL
                     AVYSLMGLRVKVNVPMCVTPAHFLLLARSAGQSREQMLRVSAAAPTNSLLGVARDCYVT
                     GTTRLYIPKEGGMVFEGLFRSPKARGNVGFVAGSSYGTGSVWTRNNEVVVLTASHVVGR
                     ANMATLKIGDAMLTLTFKKNGDFAEAVTTQSELPGNWPQLHFAQPTTGPASWCTATGDE
                     EGLLSGEVCLAWTTSGDSGSAVVQGDAVVGVHTGSNTSGVAYVTTPSGKLLGADTVTLS
                     SLSKHFTGPLTSIPKDIPDNIIADVDAVPRSLAMLIDGLSNRESSLSGPQLLLIACFMW
                     SYLNQPAYLPYVLGFFAANFFLPKSVGRPVVTGLLWLCCLFTPLSMRLCLFHLVCATVT
                     GNVISLWFYITAAGTSYLSEMWFGGYPTLLFVPRFLVYQFPGWAIGTVLAVCSITMLAA
                     ALGHTLLLDVFSASGRFDRTFMMKYFLEGGVKESVTASVTRAYGKPITQESLTATLAAL
                     TDDDFQFLSDVLDCRAVRSAMNLRAALTSFQVAQYRNILNASLQVDRDAARSRRLMAKL
                     ADFAVEQEVTAGDRVVVIDGLDRMAHFKDDLVLVPLTTKVVGGSRCTICDVVKEEANDT
                     PVKPMPSRRRRKGLPKGAQLEWDRHQEEKRNAGDDDFAVSTDYVKRVPKYWDPSDTRGT
                     TVKIAGTTYQKVVDYSGNVHYVEHQEDLLDYVLGKGSYEGLDQDKVLDLTNMLKVDPTE
                     LSSKDKAKARQLAHLLLDLANPVEAVNQLN"
     gene            225..5408
                     /gene="ORF1a"
                     /label="ORF1a"
     regulatory      6374..6383
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9824..10504
                     /gene="GP2b"
                     /label="Glycoprotein 2b"
                     /note="Glycoprotein 2b from Equine arteritis virus (strain
                     Bucyrus). Accession#: P28992"
     CDS             10700..11155
                     /gene="GP4"
                     /label="Glycoprotein 4"
                     /note="Glycoprotein 4 from Equine arteritis virus (strain
                     Bucyrus). Accession#: P28994"
     CDS             11146..11910
                     /gene="GP5"
                     /label="Glycoprotein 5"
                     /note="Glycoprotein 5 from Equine arteritis virus (strain
                     Bucyrus). Accession#: P28995"
     CDS             11901..12386
                     /gene="M"
                     /label="Membrane protein"
                     /note="Membrane protein from Equine arteritis virus (strain
                     Bucyrus). Accession#: P28991"
     promoter        12785..12889
                     /label="AmpR promoter"
     CDS             12890..13747
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      13921..14509
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"