LOCUS       V007691                14553 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007691
VERSION     V007691
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 14553)
  AUTHORS   Zhang J, Go YY, Huang CM, Meade BJ, Lu Z, Snijder EJ, Timoney PJ,
            Balasuriya UB.
  TITLE     Development and characterization of an infectious cDNA clone of the
            modified live virus vaccine strain of equine arteritis virus
  JOURNAL   Clin. Vaccine Immunol. 19 (8), 1312-1321 (2012)
   PUBMED   22739697
REFERENCE   2  (bases 1 to 14553)
  AUTHORS   Zhang J, Balasuriya UBR.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2009) Veterinary Science, University of Kentucky,
            108 Gluck Equine Research Center, Lexington, KY 40546, USA
REFERENCE   3  (bases 1 to 14553)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14553)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Clin.
            Vaccine Immunol."; date: "2012"; volume: "19"; issue: "8"; pages:
            "1312-1321"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-MAR-2009) Veterinary Science, University of Kentucky, 108 Gluck
            Equine Research Center, Lexington, KY 40546, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             225..9751
                     /codon_start=1
                     /ribosomal_slippage=""
                     /gene="ORF1ab"
                     /product="replicase polyprotein 1ab"
                     /label="ORF1ab"
                     /protein_id="ACY79514.1"
                     /translation="MATFSATGFGGSFVRDWSLDLPDACEHGAGLCCEVDGSTLCAECF
                     RGCEGVEQCPGLFMGLLKLASPVPVGHKFLIGWYRAAKVTGRYNFLELLQHPAFAQLRV
                     VDARLAIEEASVFISTDHASAKRFPGARFALTPVYANAWVVSPAANSLIVTTDQEQDGF
                     CWLKLLPPDRREAGLRLYYNHYREQRTGWLSKTGLRLWLGDLGLGINASSGGLKFHIMR
                     GSPQRAWHITTRSCKLKSYYVCDISEADWSCLPAGNYGGYNPPGDGACGYRCLAFMNGA
                     TVVSAGCSSDLWCDDELAYRVFQLSPTFTVTIPGGRVCPNAKYAMICDKQHWRVKRAKG
                     VGLCLDESCFRGTCNCQRMSGPPPAPVSAAVLDHILEAATFGNVRVVIPEGQPRPVPAP
                     RVHPCANSSGDVKDPAPVPPVPKPRTKLAKPNPTQAPIPAPRTRLQGASTQEPLASAGV
                     ASDSAPKWRVAKTVYSSAERFRTELVQRARSVGDVLVQALPHKTPAVQRYTMTLKMMRS
                     RFSWHCDMWYPLAVIACLLPIWPSLALLLSFAIGLIPSVGNNVVLTALLVSSANYVASM
                     DHHCEGAACLALLEEEHYYRAVRWRPITGALSLVLNLLGQVGYVARSTFDAAYVPCTVF
                     DLCSFAILYLCCNRCWRCFGRCVRVGPATHVLGSTGQRVSKLALIDLCDHFSKPTIDVV
                     GMATGWSGCYTGTAAMERQCASTVDPHSFDQKKAGAIVYLTPPVNSGSALQCLNVMWKR
                     PIGSTVLGEQTGAVVTAVKSISFSPPCCVSTTLPTRPGVTVVDHALYNRLTASGVDPAL
                     LRVGQGDFLKLNPGFRLIGGWIYGICYFVLVVVSTFTCLPIKCGIGTRDPFCRRVFSVP
                     VIKTQEHCHAGMCASAEGISLDSLGLTQLQSYWIAAVTSGLVILLVCHRLAISALDLLT
                     LASPLVLLVFPWASVGLLLACSLAGAAVKIQLLATLFVNLFFPQATLVTMGYWACVAAL
                     AVYSLMGLRVKVNVPMCVTPAHFLLLARSAGQSREQMLRVSAAAPTNSLLGVARDCYVT
                     GTTRLYIPKEGGMVFEGLFRSPKARGNVGFVAGSSYGTGSVWTRNNEVVVLTASHVVGR
                     ANMATLKIGDAMLTLTFKKNGDFAEAVTTQSELPGNWPQLHFAQPTTGPASWCTATGDE
                     EGLLSGEVCLAWTTSGDSGSAVVQGDAVVGVHTGSNTSGVAYVTTPSGKLLGADTVTLS
                     SLSKHFTGPLTSIPKDIPDNIIADVDAVPRSLAMLIDGLSNRESSLSGPQLLLIACFMW
                     SYLNQPAYLPYVLGFFAANFFLPKSVGRPVVTGLLWLCCLFTPLSMRLCLFHLVCATVT
                     GNVISLWFYITAAGTSYLSEMWFGGYPTLLFVPRFLVYQFPGWAIGTVLAVCSITMLAA
                     ALGHTLLLDVFSASGRFDRTFMMKYFLEGGVKESVTASVTRAYGKPITQESLTATLAAL
                     TDDDFQFLSDVLDCRAVRSAMNLRAALTSFQVAQYRNILNASLQVDRDAARSRRLMAKL
                     ADFAVEQEVTAGDRVVVIDGLDRMAHFKDDLVLVPLTTKVVGGSRCTICDVVKEEANDT
                     PVKPMPSRRRRKGLPKGAQLEWDRHQEEKRNAGDDDFAVSTDYVKRVPKYWDPSDTRGT
                     TVKIAGTTYQKVVDYSGNVHYVEHQEDLLDYVLGKGSYEGLDQDKVLDLTNMLKVDPTE
                     LSSKDKAKARQLAHLLLDLANPVEAVNQLN*ERPTSFPAMWGVGHLLTLKTRVSWLYTV
                     AQCF*LPGTFYLTSNLCATKSSQRPQKTHCLGTYAPALVTGLFSVVRTGH*LMHTGTVW
                     SAFTRFPPYLILM*AQVTSQ*RVSDGILNLPEEAVQNVSQLIWCTLFKGSTEPLIPMMT
                     RWQLLSVVTRIGRTASCITPVGATFHILSQPMLWKPQLATVLDVRPLPTGPTSSQQLGP
                     SRSNNPYRTSQRACLTTALTSAVTLS*RPLQRQPCVVI*RNTTYPRRVLCCLVFSPWCG
                     RT*KRRLETLHHSTCHLLYHLKIHKPELTALSFLQGLYRATV*LMTWCHSP*KAIYKPP
                     PWRLVNGSTVPNTRLGAFWAPTITLA*VCVPAFRGLRPHSKKLERMGHRFIWASQNSTR
                     YLLLTSTALKQTWRVVIAPPRLWCVGSLLILFLS*LASPSWCTATC*IAVTI*LWQVV*
                     HSPNAGVCHLETLSLPFPIPSIHWCCTPSTCCYVDLKAISQRLQKNILMAAWSCGTCSS
                     TFECTSTRTMWF*PHPTSITRPALTAGSPTCRRC*VSRLTQRKL*TPAPLPFWAAGSSK
                     WTASVIWPVFRTALHALCYTTLVQRIPQSTMKLLFPSLRTPLSAVMKIGGRTSIDVSVA
                     LRVLTELSSPPLKC*HPSAPSSMRVPCAQFVGPPPWPSLLVEGGSVAIVSRTTWVIVTQ
                     PRSSPTAGTTSCTAPLTAQCVRVPQNRWYQKCLTRSWIICCATLITAVKRN*LW*WRMV
                     EQHHRPGATKWVTR*SPWLQTWEATLCLGAVLDHTSQYHFRIRSRAWW*IKL*RTPPPL
                     STWKDPLGVGRLFTWSKMC*PWSVARPWLCPPTRPCWTALTSSNKRAPIHTLWCPSIQF
                     LTFPGLAVETSQCDCHRSELVREKPLWMRWLTSHQWIWRAF*PRVESRVTVI*ISSGAS
                     DPRACHVTFGSDILSAWSPCECAIDSALLCVI*SRAFILIMSQLHIPLKWCLCQIQTLR
                     KV*SSPPTTKIAVLVTAQLIQFKAVHSLL*LFDCPHPNH*RARAQLWRLLGRLRNYTST
                     TPLISLAGC*SSPRKQRRRT*STAHLQHATWAKKLTFGPMRASNITRKSTCCTHTSPSR
                     MV*YTVTLIVALPVAGKSNPTKFRASREWHKIWATTIPQIYQDFAPYRKNSLSIGP*CP
                     MIDTRIACKLPYSKYVNSVNRAQRAIWLDNLFSCRRLV*HLTGLLNGSTAKRVLYQIPY
                     SRPVGSRLTAAFSSMKPRKSLPPLTLMPVWEKLISPPWEDPTSSFPNIYHHCYPQALLP
                     W*VLHWLGKLLKLLAALLMSMLHRLNLIYTLRH*VACTRL*SISSRVGLWCGETRPFMS
                     KRVLMQLHQH*QLCPNSSKCRPMSLFHSMWHQGTEPTRW*RPRLKFQLEPTPPSGHCQL
                     NRHRLVMRSCGDIL*RGSSAQQKCSCAAGVLCLPPQCRPFVH*RDVNLCSTSYKLVQSL
                     GPC"
     gene            225..9751
                     /gene="ORF1ab"
                     /label="ORF1ab"
     CDS             225..5408
                     /codon_start=1
                     /gene="ORF1a"
                     /product="replicase polyprotein 1a"
                     /label="ORF1a"
                     /protein_id="ACY79515.1"
                     /translation="MATFSATGFGGSFVRDWSLDLPDACEHGAGLCCEVDGSTLCAECF
                     RGCEGVEQCPGLFMGLLKLASPVPVGHKFLIGWYRAAKVTGRYNFLELLQHPAFAQLRV
                     VDARLAIEEASVFISTDHASAKRFPGARFALTPVYANAWVVSPAANSLIVTTDQEQDGF
                     CWLKLLPPDRREAGLRLYYNHYREQRTGWLSKTGLRLWLGDLGLGINASSGGLKFHIMR
                     GSPQRAWHITTRSCKLKSYYVCDISEADWSCLPAGNYGGYNPPGDGACGYRCLAFMNGA
                     TVVSAGCSSDLWCDDELAYRVFQLSPTFTVTIPGGRVCPNAKYAMICDKQHWRVKRAKG
                     VGLCLDESCFRGTCNCQRMSGPPPAPVSAAVLDHILEAATFGNVRVVIPEGQPRPVPAP
                     RVHPCANSSGDVKDPAPVPPVPKPRTKLAKPNPTQAPIPAPRTRLQGASTQEPLASAGV
                     ASDSAPKWRVAKTVYSSAERFRTELVQRARSVGDVLVQALPHKTPAVQRYTMTLKMMRS
                     RFSWHCDMWYPLAVIACLLPIWPSLALLLSFAIGLIPSVGNNVVLTALLVSSANYVASM
                     DHHCEGAACLALLEEEHYYRAVRWRPITGALSLVLNLLGQVGYVARSTFDAAYVPCTVF
                     DLCSFAILYLCCNRCWRCFGRCVRVGPATHVLGSTGQRVSKLALIDLCDHFSKPTIDVV
                     GMATGWSGCYTGTAAMERQCASTVDPHSFDQKKAGAIVYLTPPVNSGSALQCLNVMWKR
                     PIGSTVLGEQTGAVVTAVKSISFSPPCCVSTTLPTRPGVTVVDHALYNRLTASGVDPAL
                     LRVGQGDFLKLNPGFRLIGGWIYGICYFVLVVVSTFTCLPIKCGIGTRDPFCRRVFSVP
                     VIKTQEHCHAGMCASAEGISLDSLGLTQLQSYWIAAVTSGLVILLVCHRLAISALDLLT
                     LASPLVLLVFPWASVGLLLACSLAGAAVKIQLLATLFVNLFFPQATLVTMGYWACVAAL
                     AVYSLMGLRVKVNVPMCVTPAHFLLLARSAGQSREQMLRVSAAAPTNSLLGVARDCYVT
                     GTTRLYIPKEGGMVFEGLFRSPKARGNVGFVAGSSYGTGSVWTRNNEVVVLTASHVVGR
                     ANMATLKIGDAMLTLTFKKNGDFAEAVTTQSELPGNWPQLHFAQPTTGPASWCTATGDE
                     EGLLSGEVCLAWTTSGDSGSAVVQGDAVVGVHTGSNTSGVAYVTTPSGKLLGADTVTLS
                     SLSKHFTGPLTSIPKDIPDNIIADVDAVPRSLAMLIDGLSNRESSLSGPQLLLIACFMW
                     SYLNQPAYLPYVLGFFAANFFLPKSVGRPVVTGLLWLCCLFTPLSMRLCLFHLVCATVT
                     GNVISLWFYITAAGTSYLSEMWFGGYPTLLFVPRFLVYQFPGWAIGTVLAVCSITMLAA
                     ALGHTLLLDVFSASGRFDRTFMMKYFLEGGVKESVTASVTRAYGKPITQESLTATLAAL
                     TDDDFQFLSDVLDCRAVRSAMNLRAALTSFQVAQYRNILNASLQVDRDAARSRRLMAKL
                     ADFAVEQEVTAGDRVVVIDGLDRMAHFKDDLVLVPLTTKVVGGSRCTICDVVKEEANDT
                     PVKPMPSRRRRKGLPKGAQLEWDRHQEEKRNAGDDDFAVSTDYVKRVPKYWDPSDTRGT
                     TVKIAGTTYQKVVDYSGNVHYVEHQEDLLDYVLGKGSYEGLDQDKVLDLTNMLKVDPTE
                     LSSKDKAKARQLAHLLLDLANPVEAVNQLN"
     gene            225..5408
                     /gene="ORF1a"
                     /label="ORF1a"
     regulatory      6374..6383
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9824..10504
                     /gene="GP2b"
                     /label="Glycoprotein 2b"
                     /note="Glycoprotein 2b from Equine arteritis virus (strain
                     Bucyrus). Accession#: P28992"
     CDS             10700..11155
                     /gene="GP4"
                     /label="Glycoprotein 4"
                     /note="Glycoprotein 4 from Equine arteritis virus (strain
                     Bucyrus). Accession#: P28994"
     CDS             11146..11910
                     /gene="GP5"
                     /label="Glycoprotein 5"
                     /note="Glycoprotein 5 from Equine arteritis virus (strain
                     Bucyrus). Accession#: P28995"
     CDS             11901..12386
                     /gene="M"
                     /label="Membrane protein"
                     /note="Membrane protein from Equine arteritis virus (strain
                     Bucyrus). Accession#: P28991"
     promoter        12785..12889
                     /label="AmpR promoter"
     CDS             12890..13747
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      13921..14509
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gctcgaagtg tgtatggtgc catatacggc tcaccgccat atgcactgca agaattacta
       61 ttcttgtggg cccctctcgg taaatcctag agggctttcc tctcgttatt gcgagattcg
      121 tcgttagata acggcaagtt ccctttctta ctatcctatt ttcatcttgt ggcttgacgg
      181 gtcactgcca tcgtcgtcga tctctatcaa ctacccttgc gactatggca accttctccg
      241 ctactggatt tggagggagt tttgttaggg actggtccct ggacttaccc gacgcttgtg
      301 agcatggcgc gggattgtgc tgtgaagtgg acggctccac cttatgcgcc gagtgttttc
      361 gcggttgcga aggagtggag caatgtcctg gcttgttcat gggactgtta aaactggctt
      421 cgccagttcc agtgggacat aagttcctga ttggttggta tcgagctgcc aaagtcaccg
      481 ggcgttacaa tttccttgag ctgttgcaac accctgcttt cgcccagctg cgtgtggttg
      541 atgctaggtt agccattgaa gaggcaagtg tgtttatttc cactgaccac gcgtctgcta
      601 agcgtttccc tggcgctaga tttgcgctga caccggtgta tgctaacgct tgggttgtga
      661 gcccggctgc taacagtttg atagtgacca ctgaccagga acaagatggg ttctgctggt
      721 taaaactttt accacctgac cgccgtgagg ctggtttgcg gttgtattac aaccattacc
      781 gcgaacaaag gaccgggtgg ctgtctaaaa caggacttcg cttatggctt ggagacctgg
      841 gtttgggcat caatgcgagc tctggagggc tgaaattcca cattatgagg ggttcgcctc
      901 agcgagcttg gcatatcaca acacgcagct gcaagctgaa gagctactac gtttgtgaca
      961 tctctgaagc agactggtcc tgtttgcccg ctggcaacta cggcggctac aatccaccag
     1021 gggacggagc ttgcggttac aggtgcttgg ccttcatgaa tggcgccact gttgtgtcgg
     1081 ctggttgcag ttctgacttg tggtgtgatg atgagttggc ttatcgagtc tttcaattgt
     1141 cacccacgtt cacggttacc atcccaggtg ggcgagtttg tccgaatgcc aagtacgcaa
     1201 tgatttgtga caagcagcac tggcgcgtca aacgtgcaaa gggcgtcggc ctgtgtctcg
     1261 atgaaagctg tttcaggggc acctgcaatt gccaacgcat gagtggacca ccacctgcac
     1321 ccgtgtcagc cgccgtgtta gatcacatac tggaggcggc gacgtttggc aacgttcgcg
     1381 tggttatacc tgaagggcag ccacgccccg taccagcgcc gcgagttcat ccctgcgcca
     1441 actcttctgg agatgtcaaa gatccggcgc ccgttccgcc agtaccaaaa ccaaggacca
     1501 agcttgccaa accgaaccca actcaggcgc ccatcccagc accgcgcacg cgacttcaag
     1561 gggcctcaac acaggagcca ctggcgagtg cgggagttgc ttctgactcg gcacccaaat
     1621 ggcgtgtggc caaaactgtg tacagctccg cggagcgctt tcggaccgaa ctggtacaac
     1681 gtgctcggtc cgttggggac gttcttgttc aagcgctacc gcacaaaacc ccagcagtgc
     1741 agcggtatac catgactctg aagatgatgc gttcacgctt cagttggcac tgcgacatgt
     1801 ggtacccttt ggctgtaatc gcttgtttgc tccccatatg gccatctctt gctttgctcc
     1861 ttagcttcgc cattgggttg atacccagtg tgggcaataa tgttgttctg acagcgcttc
     1921 tggtttcatc agctaattat gttgcgtcaa tggaccatca ctgtgaaggt gcggcttgct
     1981 tagccttgct ggaagaagaa cactattata gagcggtccg ttggcgcccg attacaggcg
     2041 cgctgtcgct tgtgctcaat ttactggggc aggtaggcta tgtagctcgt tccacctttg
     2101 atgcagctta tgttccttgc actgtgttcg atctttgcag ctttgctatt ctgtacctct
     2161 gctgcaatcg ttgctggaga tgcttcggac gctgtgtgcg agttgggcct gccacgcatg
     2221 ttttgggttc caccgggcaa cgagtttcca aactggcgct cattgatttg tgtgaccact
     2281 tttcaaagcc caccatcgac gttgtgggca tggcaactgg ttggagcgga tgttacacag
     2341 gaaccgccgc aatggagcgt cagtgtgcct ctacggtgga ccctcactcg ttcgaccaga
     2401 agaaggcagg agcgattgtt tacctcaccc cccctgtcaa cagcgggtct gcgctgcagt
     2461 gcctcaatgt catgtggaag cgaccaattg ggtccactgt ccttggggaa caaacaggag
     2521 ctgttgtgac ggcggtcaag agcatctctt tctcacctcc ctgctgcgtc tctaccactt
     2581 tgcccacccg acccggtgtg accgttgtcg accatgctct ttacaaccgg ttgactgctt
     2641 caggggtcga tcccgcttta ttgcgtgttg ggcaaggtga ttttctaaaa cttaatccgg
     2701 ggttccggct gataggtgga tggatttatg ggatatgcta ttttgtgttg gtggttgtgt
     2761 caacttttac ctgcctacct atcaaatgtg gcattggcac ccgcgaccct ttctgccgca
     2821 gagtgttttc tgtacccgtc atcaagaccc aagagcactg ccatgctgga atgtgtgcta
     2881 gcgctgaagg catctctctg gactctctgg ggttaactca gttacaaagt tattggatcg
     2941 cagccgtcac tagcggatta gtgatcttgt tggtctgcca ccgcctggcc atcagcgcct
     3001 tggacttgtt gactctagct tcccctttag tgttgcttgt gttcccttgg gcatctgtgg
     3061 ggcttttact tgcttgcagt ctcgctggtg ctgctgtgaa aatacagttg ttggcgacgc
     3121 tttttgtgaa tctgttcttt ccccaagcta cccttgtcac tatgggatac tgggcgtgcg
     3181 tggcggcttt ggccgtttac agtttgatgg gcttgcgagt gaaagtgaat gtgcccatgt
     3241 gtgtgacacc tgcccatttt ctgctgctgg cgaggtcagc tggacagtca agagagcaga
     3301 tgctccgggt cagcgctgct gcccccacca attcactgct tggagtggct cgtgattgtt
     3361 atgtcacagg cacaactcgg ctgtatatac ccaaggaagg cgggatggtg tttgaagggc
     3421 tattcaggtc accgaaggcg cgcggcaacg tcggcttcgt ggctggtagc agctacggca
     3481 cagggtcagt gtggaccagg aacaacgagg tcgtcgtact gacagcgtca cacgtggttg
     3541 gccgcgctaa catggccact ctgaagatcg gtgacgcaat gctgactctg actttcaaaa
     3601 aaaatggcga cttcgccgag gcagtgacga cacagtccga gctcccaggc aattggccac
     3661 agttgcattt cgcccaacca acaaccgggc ccgcttcatg gtgcactgcc acaggagatg
     3721 aagaaggctt gctcagtggc gaggtttgtc tggcgtggac tactagtggc gactctggat
     3781 cagcagtggt tcagggtgac gctgtggtag gggtccacac cggttcgaac acaagtggtg
     3841 ttgcctacgt gaccacccca agcggaaaac tccttggcgc cgacaccgtg actttgtcgt
     3901 cactgtcaaa gcatttcaca ggccctttga catcaatccc gaaggacatc cctgacaaca
     3961 tcattgccga tgttgatgct gttcctcgtt ctctggccat gctgattgat ggcttatcta
     4021 atagagagag cagcctttct ggacctcagt tgttgttaat tgcttgtttt atgtggtctt
     4081 atcttaacca acctgcctac ttgccttatg tgctgggctt ctttgccgct aacttcttcc
     4141 tgccaaaaag tgttggccgc cctgtggtca ctgggcttct atggttgtgc tgcctcttca
     4201 caccgctttc catgcgcttg tgcttgttcc atctggtctg tgctaccgtc acgggaaacg
     4261 tgatatcttt gtggttctac atcactgccg ctggcacgtc ttacctttct gagatgtggt
     4321 tcggaggcta tcccaccttg ttgtttgtgc cacggttcct agtgtaccag ttccccggct
     4381 gggctattgg cacagtacta gcggtatgca gcatcaccat gctggctgct gccctcggtc
     4441 acaccctgtt actggatgtg ttctccgcct cgggtcgctt tgacaggact ttcatgatga
     4501 aatacttcct ggagggagga gtgaaagaga gtgtcaccgc ctcagtcacc cgcgcttatg
     4561 gcaaaccaat tacccaggag agtctcactg caacattagc tgccctcact gatgatgact
     4621 tccaattcct ctctgatgtg cttgactgtc gggccgtccg atcggcaatg aatctgcgtg
     4681 ccgctctcac aagttttcaa gtggcgcagt atcgtaacat ccttaatgca tccttgcaag
     4741 tcgatcgtga cgctgctcgt agtcgcagac taatggcaaa actggctgat tttgcggttg
     4801 aacaagaagt aacagccgga gaccgtgttg ttgttatcga cggtctggac cgcatggctc
     4861 acttcaaaga cgatttggtg ctggttcctt tgaccaccaa agtagtaggc ggttctaggt
     4921 gcaccatttg tgacgtcgtt aaggaagaag ccaatgacac cccagttaag ccaatgccca
     4981 gcaggagacg ccgcaagggc ctgcctaaag gtgctcagtt ggagtgggac cgtcaccagg
     5041 aagagaagag gaacgccggt gatgatgatt ttgcggtctc gactgattat gtcaagagag
     5101 tgccaaagta ctgggatccc agcgacaccc gaggcacgac agtgaaaatc gccggcacta
     5161 cctatcagaa agtggttgac tattcaggca atgtgcatta cgtggagcat caggaagatc
     5221 tgctagacta cgtgctgggc aaggggagct atgaaggcct agatcaggac aaagtgttgg
     5281 acctcacaaa catgcttaaa gtggacccca cggagctctc ctccaaagac aaagccaagg
     5341 cgcgtcagct tgctcatctg ctgttggatc tggctaaccc agttgaggca gtgaatcagt
     5401 taaactgaga gcgccccaca tctttcccgg cgatgtgggg cgtcggacat ttgctgactc
     5461 taaagacaag ggtttcgtgg ctctacacag tcgcacaatg tttttagctg cccgggactt
     5521 tttatttaac atcaaatttg tgtgcgacga agagttcaca aagaccccaa aagacacact
     5581 gcttgggtac gtacgcgcct gccctggtta ctggtttatt ttccgtcgta cgcaccggtc
     5641 actgattgat gcatactggg acagtatgga gtgcgtttac gcgcttccca ccatatctga
     5701 ttttgatgtg agcccaggtg acgtcgcagt gacgggtgag cgatgggatt ttgaatctcc
     5761 cggaggaggc cgtgcaaaac gtctcacagc tgatctggtg cacgcttttc aagggttcca
     5821 cggagcctct tattcctatg atgacaaggt ggcagctgct gtcagtggtg acccgtatcg
     5881 gtcggacggc gtcttgtata acacccgttg gggcaacatt ccatattctg tcccaaccaa
     5941 tgctttggaa gccacagctt gctaccgtgc tggatgtgag gccgttaccg acgggaccaa
     6001 cgtcatcgca acaattgggc ccttcccgga gcaacaaccc ataccggaca tcccaaagag
     6061 cgtgcttgac aactgcgctg acatcagctg tgacgctttc atagcgcccg ctgcagagac
     6121 agccctgtgt ggtgatttag agaaatacaa cctatccacg cagggttttg tgttgcctag
     6181 tgttttctcc atggtgcggg cgtacttaaa agaggagatt ggagacgctc caccactcta
     6241 cttgccatct actgtaccat ctaaaaattc acaagccgga attaacggcg ctgagtttcc
     6301 tacaaggtct ttacagagct actgtttgat tgatgacatg gtgtcacagt ccatgaaaag
     6361 caatctacaa accgccacca tggcgacttg taaacggcag tactgttcca aatacaagat
     6421 taggagcatt ctgggcacca acaattacat tggcctaggt ttgcgtgcct gcctttcggg
     6481 ggttacggcc gcattccaaa aagctggaaa ggatgggtca ccgatttatt tgggcaagtc
     6541 aaaattcgac ccgatacctg ctcctgacaa gtactgcctt gaaacagacc tggagagttg
     6601 tgatcgctcc accccggctt tggtgcgttg gttcgctact aatcttattt ttgagctagc
     6661 tggccagccc gagttggtgc acagctacgt gttgaattgc tgtcacgatc tagttgtggc
     6721 aggtagtgta gcattcacca aacgcggggg tttgtcatct ggagacccta tcacttccat
     6781 ttccaatacc atctattcat tggtgctgta cacccagcac atgttgctat gtggacttga
     6841 aggctatttc ccagagattg cagaaaaata tcttgatggc agcctggagc tgcgggacat
     6901 gttcaagtac gttcgagtgt acatctactc ggacgatgtg gttctaacca cacccaacca
     6961 gcattacgcg gccagctttg accgctgggt cccccacctg caggcgctgc taggtttcaa
     7021 ggttgaccca aagaaaactg tgaacaccag ctccccttcc tttttgggct gccggttcaa
     7081 gcaagtggac ggcaagtgtt atctggccag tcttcaggac cgcgttacac gctctctgtt
     7141 ataccacatt ggtgcaaaga atccctcaga gtactatgaa gctgctgttt ccatctttaa
     7201 ggactccatt atctgctgtg atgaagattg gtggacggac ctccatcgac gtatcagtgg
     7261 cgctgcgcgt actgacggag ttgagttccc caccattgaa atgttaacat ccttccgcac
     7321 caagcagtat gagagtgccg tgtgcacagt ttgtggggcc gcccccgtgg ccaagtctgc
     7381 ttgtggaggg tggttctgtg gcaattgtgt cccgtaccac gtgggtcatt gtcacacaac
     7441 ctcgctcttc gccaactgcg ggcacgacat catgtaccgc tccacttact gcacaatgtg
     7501 tgagggttcc ccaaaacaga tggtaccaaa agtgcctcac ccgatcctgg atcatttgct
     7561 gtgccacgtt gattacggca gtaaagagga actaactctg gtagtggcgg atggtcgaac
     7621 aacatcaccg cccgggcgct acaaagtggg tcacaaggta gtcgccgtgg ttgcagacgt
     7681 gggaggcaac attgtgtttg ggtgcggtcc tggatcacac atcgcagtac cacttcagga
     7741 tacgctcaag ggcgtggtgg tgaataaagc tctgaagaac gccgccgcct ctgagtacgt
     7801 ggaaggaccc cctgggagtg ggaagacttt tcacctggtc aaagatgtgc tagccgtggt
     7861 cggtagcgcg accttggttg tgcccaccca cgcgtccatg ctggactgca ttaacaagct
     7921 caaacaagcg ggcgccgatc catactttgt ggtgcccaag tatacagttc ttgactttcc
     7981 ccggcctggc agtggaaaca tcacagtgcg actgccacag gtcggaacta gtgagggaga
     8041 aacctttgtg gatgaggtgg cttacttctc accagtggat ctggcgcgca ttttaaccca
     8101 gggtcgagtc aagggttacg gtgatttaaa tcagctcggg tgcgtcggac ccgcgagcgt
     8161 gccacgtaac ctttggctcc gacattttgt cagcctggag cccttgcgag tgtgccatcg
     8221 attcggcgct gctgtgtgtg atttgatcaa gggcatttat ccttattatg agccagctcc
     8281 acataccact aaagtggtgt ttgtgccaaa tccagacttt gagaaaggtg tagtcatcac
     8341 cgcctaccac aaagatcgcg gtcttggtca ccgcacaatt gattcaattc aaggctgtac
     8401 attccctgtt gtgactcttc gactgcccac accccaatca ctgacgcgcc cgcgcgcagt
     8461 tgtggcggtt actagggcgt ctcaggaatt atacatctac gacccctttg atcagcttag
     8521 cgggttgttg aagttcacca aggaagcaga ggcgcaggac ttgatccacg gcccacctac
     8581 agcatgccac ctgggccaag aaattgacct ttggtccaat gagggcctcg aatattacaa
     8641 ggaagtcaac ctgctgtaca cacacgtccc catcaaggat ggtgtaatac acagttaccc
     8701 taattgtggc cctgcctgtg gctgggaaaa gcaatccaac aaaatttcgt gcctcccgag
     8761 agtggcacaa aatttgggct accactattc cccagattta ccaggatttt gccccatacc
     8821 gaaagaactc gctgagcatt ggcccgtagt gtccaatgat agatacccga attgcttgca
     8881 aattacctta cagcaagtat gtgaactcag taaaccgtgc tcagcgggct atatggttgg
     8941 acaatctgtt ttcgtgcaga cgcctggtgt gacatcttac tggcttactg aatgggtcga
     9001 cggcaaagcg cgtgctctac cagattcctt attctcgtcc ggtaggttcg agactaacag
     9061 ccgcgttttc ctcgatgaag ccgaggaaaa gtttgccgcc gctcaccctc atgcctgttt
     9121 gggagaaatt aataagtcca ccgtgggagg atcccacttc atcttttccc aatatttacc
     9181 accactgcta cccgcaggcg ctgttgccct ggtaggtgct tcattggctg ggaaagctgc
     9241 taaagctgct tgcagcgttg ttgatgtcta tgctccatcg tttgaacctt atctacaccc
     9301 tgagacactg agtcgcgtgt acaagattat gatcgatttc aagccgtgta ggcttatggt
     9361 gtggagaaac gcgacctttt atgtccaaga gggtgttgat gcagttacat cagcactagc
     9421 agctgtgtcc aaactcatca aagtgccggc caatgagcct gtttcattcc atgtggcatc
     9481 agggtacaga accaacgcgc tggtagcgcc ccaggctaaa atttcaattg gagcctacgc
     9541 cgccgagtgg gcactgtcaa ctgaaccgcc accggctggt tatgcgatcg tgcggcgata
     9601 tattgtaaag aggctcctca gctcaacaga agtgttcttg tgccgcaggg gtgttgtgtc
     9661 ttccacctca gtgcagacca tttgtgcact agagggatgt aaacctctgt tcaacttctt
     9721 acaaattggt tcagtcattg ggcccgtgtg atgggcttag tgtggtcact gatttcaaat
     9781 tctattcaga ctattattgc tgattttgct atttctgtga ttgatgcagc gcttttcttt
     9841 ctcatgctac ttgcattggc tgttgttact gtgtttcttt tctggctcat tgttgccatc
     9901 ggccgcagct tggtggcgcg gtgttcacga ggtgcgcgtt acagacctgt ttaaggattt
     9961 gcagtgcgac aacctgcgcg cgaaagatgc cttcccgagt ctgggacatg ctctgtcgat
    10021 tggccagtcg aggctatcgt atatgctgca ggattggttg cttgctgcgc accgcaagga
    10081 agttatgcct tccaatacca tgcctatgcc cggtcttact cctgattgct ttgaccatct
    10141 ggagtcttct agctatgctc catttatcaa tgcctatcgg caggcaattt tgagtcaata
    10201 ctcacaagag ctcctgctcg aagccatcaa ctgtaaattg cttgctgtgg ttgcaccggc
    10261 attgtatcac aattaccatc tagccaattt gaccgaaccg gccacatggg tcgtgcctac
    10321 agtgggccag ttgcactatt atgcttcttc ctctattttt gcttcatctg tggaagtgtt
    10381 ggcagcaata atactactat ttgcatgcat accactagtg acacgagtgt acatctcttt
    10441 tacgcggcta atgtcacctt cccgtcgcac ttccagcggc actttgccgc cgcgcaagat
    10501 tttgtagtgc acacgggtta tgaatatgcc ggggtcacta tggtagtgca cttgtttgcc
    10561 aacttggttc tgacatttcc gagcttagtt aattgttccc gccctgtgaa tgtctttgct
    10621 aatgcttctt gcgtgcaagt ggtttgtagt cataccaact caactactgg ctcgggtcaa
    10681 ctttcgtttt cctttgtaga tgaagatctc cggctgcata tcaggcctac tcttatttgt
    10741 tggtttgcct tgttgttggt gcactttcta cccatgccac gctacagagg ctcgcaattt
    10801 tacttacact agtcatggat tgggccacgt gcacggtcat gaggggtgta ggaattttat
    10861 taatgtcact cattctgcat ttctttatct taatcccacc actctcactg cgccggctat
    10921 aactcattgt ttacttctgg ttctggcagc caaaatggaa cacccaaacg ctactatctg
    10981 gctgcagctg cagccgtttg ggtatcatgt ggctggcgat gtcattgtca acttggaaga
    11041 gaataagagg catccttact ttaaactctt gagagcgccg gctttaccgc ttggttttgt
    11101 ggctatagtt tatgttcttt tacgactggt acgttgggct caacaatgct atctatgatt
    11161 gtattgttat tcttgctttg gggtgcgcca tcacatgctt acttctcata ctacaccgct
    11221 cagcgcttca cagacttcac cttgtgcatg ctgacggatc gcggcgttat tgccaatttg
    11281 ctgcgatatg atgagcacac tgctttgtac aattgttccg ccagtaaaac ctgttggtat
    11341 tgcacattcc cggacgaaaa gattatcacg tttggaaccg attgtgatga cacctacgcg
    11401 gtcccagttg ctgaggtcct ggaacaggcg catggaccgt acggtgtgct gtttggtgac
    11461 gtgccccctt ttatttacta tggccgtgaa ttcggcatag ttgtgttgga tgtgtttatg
    11521 ttctatcccg ttttagttct gtttttctta tcagtactac cctatgctac gcttattctt
    11581 gaaatgtgtg tatctattct gtttataatc tatggcattt acagcggggc ctacttggcc
    11641 atgggcatat tttcggccac gcttgctata cattcaattg tggtcctccg ccaattactg
    11701 tggttatgcc tggcttggcg ataccgctgc acgcttcacg cgtcctttat atcagctgag
    11761 gggaaagtgt accccgtaga ccccgaactc ccggttgccg ccgcgggcaa tcggttgcta
    11821 gtcccaggta ggcccactat cgattatgca gtggcctacg gcagcaaagt caaccttgtg
    11881 aggttggggg cagctgaggt atgggagcca tagattcatt ttgtggtgac gggattttag
    11941 gtgagtatct agattacttt attctgtccg tcccactctt gctgttgctt actaggtatg
    12001 tagcatctgg gtcagtgtat gttttgactg ccttgttcta ttccttagta ttagcagctt
    12061 atatttggtt tgtcatagtt ggaagagcct tttccactgc ttatgctttt gcgcttttgg
    12121 ctgcttttct gttattagta acgaggatga ttgtaggtat gatgcctcgt cttcggtcca
    12181 ttttcaacca tcgccaactg gtggtagctg attttgtgga cacacctagt ggacctgttc
    12241 ccatcccccg ctcaactact caggtagtgg ttcgcggcaa cgggtacacc gcagttggta
    12301 acaagcttgt cgatggcgtc aagacgatca cgtccgcagg ccgcctctgt tcgaaacgga
    12361 tggcggcgac agcctacaag ctacaatgac ctactgcgca tgtttggtca gatgcgggtc
    12421 cggaaaccgc ccgcgcaacc cactcaggct attattgcag agcctggaga ccttaggcat
    12481 gatttaaatc aacaggagcg cgccaccctt tcgtcgaacg tacaacggtt cttcatgatt
    12541 gggcatggtt cactcactgc agatgccgga ggactcacgt acaccgtcag ttgggttcct
    12601 accaaacaaa tccagcgcaa aattgcgcct ccagcagggc cgtaagacgt ggatattctc
    12661 ctgtgtggcg tcatgttgaa gtagttatta gccacccagg aaccaaaaaa aaaaaaaaaa
    12721 aaaactcgag gggaattaat tcttgaagac gaaagggcca ggtggcactt ttcggggaaa
    12781 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
    12841 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
    12901 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
    12961 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
    13021 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
    13081 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc
    13141 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
    13201 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
    13261 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
    13321 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
    13381 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
    13441 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
    13501 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
    13561 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
    13621 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
    13681 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
    13741 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
    13801 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
    13861 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
    13921 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
    13981 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
    14041 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
    14101 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
    14161 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
    14221 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
    14281 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
    14341 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
    14401 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
    14461 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
    14521 cgcgagctct ctagattaat acgactcact ata
//
