pEAVrMLVB-1b2a-MCS vector (V007692)

LOCUS       V007692                14622 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007692
VERSION     V007692
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 14622)
  AUTHORS   Zhang J, Go YY, Lu Z, Meade BJ, Timoney PJ, Balasuriya UBR.
  TITLE     Development and Characterization of an Infectious cDNA Clone of the
            Modified Live Virus Vaccine Strain of Equine Arteritis Virus and Its
            Potential As a Vaccine Vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14622)
  AUTHORS   Zhang J, Balasuriya UBR.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2010) Veterinary Science, University of Kentucky,
            108 Gluck Equine Research Center, Lexington, KY 40546, USA
REFERENCE   3  (bases 1 to 14622)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14622)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-JUN-2010) Veterinary Science, University of Kentucky, 108 Gluck
            Equine Research Center, Lexington, KY 40546, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             225..9751
                     /codon_start=1
                     /ribosomal_slippage=""
                     /product="replicase polyprotein 1ab"
                     /label="replicase polyprotein 1ab"
                     /note="ORF1ab"
                     /protein_id="AEG80297.1"
                     /translation="MATFSATGFGGSFVRDWSLDLPDACEHGAGLCCEVDGSTLCAECF
                     RGCEGVEQCPGLFMGLLKLASPVPVGHKFLIGWYRAAKVTGRYNFLELLQHPAFAQLRV
                     VDARLAIEEASVFISTDHASAKRFPGARFALTPVYANAWVVSPAANSLIVTTDQEQDGF
                     CWLKLLPPDRREAGLRLYYNHYREQRTGWLSKTGLRLWLGDLGLGINASSGGLKFHIMR
                     GSPQRAWHITTRSCKLKSYYVCDISEADWSCLPAGNYGGYNPPGDGACGYRCLAFMNGA
                     TVVSAGCSSDLWCDDELAYRVFQLSPTFTVTIPGGRVCPNAKYAMICDKQHWRVKRAKG
                     VGLCLDESCFRGTCNCQRMSGPPPAPVSAAVLDHILEAATFGNVRVVIPEGQPRPVPAP
                     RVHPCANSSGDVKDPAPVPPVPKPRTKLAKPNPTQAPIPAPRTRLQGASTQEPLASAGV
                     ASDSAPKWRVAKTVYSSAERFRTELVQRARSVGDVLVQALPHKTPAVQRYTMTLKMMRS
                     RFSWHCDMWYPLAVIACLLPIWPSLALLLSFAIGLIPSVGNNVVLTALLVSSANYVASM
                     DHHCEGAACLALLEEEHYYRAVRWRPITGALSLVLNLLGQVGYVARSTFDAAYVPCTVF
                     DLCSFAILYLCCNRCWRCFGRCVRVGPATHVLGSTGQRVSKLALIDLCDHFSKPTIDVV
                     GMATGWSGCYTGTAAMERQCASTVDPHSFDQKKAGAIVYLTPPVNSGSALQCLNVMWKR
                     PIGSTVLGEQTGAVVTAVKSISFSPPCCVSTTLPTRPGVTVVDHALYNRLTASGVDPAL
                     LRVGQGDFLKLNPGFRLIGGWIYGICYFVLVVVSTFTCLPIKCGIGTRDPFCRRVFSVP
                     VIKTQEHCHAGMCASAEGISLDSLGLTQLQSYWIAAVTSGLVILLVCHRLAISALDLLT
                     LASPLVLLVFPWASVGLLLACSLAGAAVKIQLLATLFVNLFFPQATLVTMGYWACVAAL
                     AVYSLMGLRVKVNVPMCVTPAHFLLLARSAGQSREQMLRVSAAAPTNSLLGVARDCYVT
                     GTTRLYIPKEGGMVFEGLFRSPKARGNVGFVAGSSYGTGSVWTRNNEVVVLTASHVVGR
                     ANMATLKIGDAMLTLTFKKNGDFAEAVTTQSELPGNWPQLHFAQPTTGPASWCTATGDE
                     EGLLSGEVCLAWTTSGDSGSAVVQGDAVVGVHTGSNTSGVAYVTTPSGKLLGADTVTLS
                     SLSKHFTGPLTSIPKDIPDNIIADVDAVPRSLAMLIDGLSNRESSLSGPQLLLIACFMW
                     SYLNQPAYLPYVLGFFAANFFLPKSVGRPVVTGLLWLCCLFTPLSMRLCLFHLVCATVT
                     GNVISLWFYITAAGTSYLSEMWFGGYPTLLFVPRFLVYQFPGWAIGTVLAVCSITMLAA
                     ALGHTLLLDVFSASGRFDRTFMMKYFLEGGVKESVTASVTRAYGKPITQESLTATLAAL
                     TDDDFQFLSDVLDCRAVRSAMNLRAALTSFQVAQYRNILNASLQVDRDAARSRRLMAKL
                     ADFAVEQEVTAGDRVVVIDGLDRMAHFKDDLVLVPLTTKVVGGSRCTICDVVKEEANDT
                     PVKPMPSRRRRKGLPKGAQLEWDRHQEEKRNAGDDDFAVSTDYVKRVPKYWDPSDTRGT
                     TVKIAGTTYQKVVDYSGNVHYVEHQEDLLDYVLGKGSYEGLDQDKVLDLTNMLKVDPTE
                     LSSKDKAKARQLAHLLLDLANPVEAVNQLN*ERPTSFPAMWGVGHLLTLKTRVSWLYTV
                     AQCF*LPGTFYLTSNLCATKSSQRPQKTHCLGTYAPALVTGLFSVVRTGH*LMHTGTVW
                     SAFTRFPPYLILM*AQVTSQ*RVSDGILNLPEEAVQNVSQLIWCTLFKGSTEPLIPMMT
                     RWQLLSVVTRIGRTASCITPVGATFHILSQPMLWKPQLATVLDVRPLPTGPTSSQQLGP
                     SRSNNPYRTSQRACLTTALTSAVTLS*RPLQRQPCVVI*RNTTYPRRVLCCLVFSPWCG
                     RT*KRRLETLHHSTCHLLYHLKIHKPELTALSFLQGLYRATV*LMTWCHSP*KAIYKPP
                     PWRLVNGSTVPNTRLGAFWAPTITLA*VCVPAFRGLRPHSKKLERMGHRFIWASQNSTR
                     YLLLTSTALKQTWRVVIAPPRLWCVGSLLILFLS*LASPSWCTATC*IAVTI*LWQVV*
                     HSPNAGVCHLETLSLPFPIPSIHWCCTPSTCCYVDLKAISQRLQKNILMAAWSCGTCSS
                     TFECTSTRTMWF*PHPTSITRPALTAGSPTCRRC*VSRLTQRKL*TPAPLPFWAAGSSK
                     WTASVIWPVFRTALHALCYTTLVQRIPQSTMKLLFPSLRTPLSAVMKIGGRTSIDVSVA
                     LRVLTELSSPPLKC*HPSAPSSMRVPCAQFVGPPPWPSLLVEGGSVAIVSRTTWVIVTQ
                     PRSSPTAGTTSCTAPLTAQCVRVPQNRWYQKCLTRSWIICCATLITAVKRN*LW*WRMV
                     EQHHRPGATKWVTR*SPWLQTWEATLCLGAVLDHTSQYHFRIRSRAWW*IKL*RTPPPL
                     STWKDPLGVGRLFTWSKMC*PWSVARPWLCPPTRPCWTALTSSNKRAPIHTLWCPSIQF
                     LTFPGLAVETSQCDCHRSELVREKPLWMRWLTSHQWIWRAF*PRVESRVTVI*ISSGAS
                     DPRACHVTFGSDILSAWSPCECAIDSALLCVI*SRAFILIMSQLHIPLKWCLCQIQTLR
                     KV*SSPPTTKIAVLVTAQLIQFKAVHSLL*LFDCPHPNH*RARAQLWRLLGRLRNYTST
                     TPLISLAGC*SSPRKQRRRT*STAHLQHATWAKKLTFGPMRASNITRKSTCCTHTSPSR
                     MV*YTVTLIVALPVAGKSNPTKFRASREWHKIWATTIPQIYQDFAPYRKNSLSIGP*CP
                     MIDTRIACKLPYSKYVNSVNRAQRAIWLDNLFSCRRLV*HLTGLLNGSTAKRVLYQIPY
                     SRPVGSRLTAAFSSMKPRKSLPPLTLMPVWEKLISPPWEDPTSSFPNIYHHCYPQALLP
                     W*VLHWLGKLLKLLAALLMSMLHRLNLIYTLRH*VACTRL*SISSRVGLWCGETRPFMS
                     KRVLMQLHQH*QLCPNSSKCRPMSLFHSMWHQGTEPTRW*RPRLKFQLEPTPPSGHCQL
                     NRHRLVMRSCGDIL*RGSSAQQKCSCAAGVLCLPPQCRPFVH*RDVNLCSTSYKLVQSL
                     GPC"
     CDS             225..5408
                     /codon_start=1
                     /product="replicase polyprotein 1a"
                     /label="replicase polyprotein 1a"
                     /note="ORF1a"
                     /protein_id="AEG80298.1"
                     /translation="MATFSATGFGGSFVRDWSLDLPDACEHGAGLCCEVDGSTLCAECF
                     RGCEGVEQCPGLFMGLLKLASPVPVGHKFLIGWYRAAKVTGRYNFLELLQHPAFAQLRV
                     VDARLAIEEASVFISTDHASAKRFPGARFALTPVYANAWVVSPAANSLIVTTDQEQDGF
                     CWLKLLPPDRREAGLRLYYNHYREQRTGWLSKTGLRLWLGDLGLGINASSGGLKFHIMR
                     GSPQRAWHITTRSCKLKSYYVCDISEADWSCLPAGNYGGYNPPGDGACGYRCLAFMNGA
                     TVVSAGCSSDLWCDDELAYRVFQLSPTFTVTIPGGRVCPNAKYAMICDKQHWRVKRAKG
                     VGLCLDESCFRGTCNCQRMSGPPPAPVSAAVLDHILEAATFGNVRVVIPEGQPRPVPAP
                     RVHPCANSSGDVKDPAPVPPVPKPRTKLAKPNPTQAPIPAPRTRLQGASTQEPLASAGV
                     ASDSAPKWRVAKTVYSSAERFRTELVQRARSVGDVLVQALPHKTPAVQRYTMTLKMMRS
                     RFSWHCDMWYPLAVIACLLPIWPSLALLLSFAIGLIPSVGNNVVLTALLVSSANYVASM
                     DHHCEGAACLALLEEEHYYRAVRWRPITGALSLVLNLLGQVGYVARSTFDAAYVPCTVF
                     DLCSFAILYLCCNRCWRCFGRCVRVGPATHVLGSTGQRVSKLALIDLCDHFSKPTIDVV
                     GMATGWSGCYTGTAAMERQCASTVDPHSFDQKKAGAIVYLTPPVNSGSALQCLNVMWKR
                     PIGSTVLGEQTGAVVTAVKSISFSPPCCVSTTLPTRPGVTVVDHALYNRLTASGVDPAL
                     LRVGQGDFLKLNPGFRLIGGWIYGICYFVLVVVSTFTCLPIKCGIGTRDPFCRRVFSVP
                     VIKTQEHCHAGMCASAEGISLDSLGLTQLQSYWIAAVTSGLVILLVCHRLAISALDLLT
                     LASPLVLLVFPWASVGLLLACSLAGAAVKIQLLATLFVNLFFPQATLVTMGYWACVAAL
                     AVYSLMGLRVKVNVPMCVTPAHFLLLARSAGQSREQMLRVSAAAPTNSLLGVARDCYVT
                     GTTRLYIPKEGGMVFEGLFRSPKARGNVGFVAGSSYGTGSVWTRNNEVVVLTASHVVGR
                     ANMATLKIGDAMLTLTFKKNGDFAEAVTTQSELPGNWPQLHFAQPTTGPASWCTATGDE
                     EGLLSGEVCLAWTTSGDSGSAVVQGDAVVGVHTGSNTSGVAYVTTPSGKLLGADTVTLS
                     SLSKHFTGPLTSIPKDIPDNIIADVDAVPRSLAMLIDGLSNRESSLSGPQLLLIACFMW
                     SYLNQPAYLPYVLGFFAANFFLPKSVGRPVVTGLLWLCCLFTPLSMRLCLFHLVCATVT
                     GNVISLWFYITAAGTSYLSEMWFGGYPTLLFVPRFLVYQFPGWAIGTVLAVCSITMLAA
                     ALGHTLLLDVFSASGRFDRTFMMKYFLEGGVKESVTASVTRAYGKPITQESLTATLAAL
                     TDDDFQFLSDVLDCRAVRSAMNLRAALTSFQVAQYRNILNASLQVDRDAARSRRLMAKL
                     ADFAVEQEVTAGDRVVVIDGLDRMAHFKDDLVLVPLTTKVVGGSRCTICDVVKEEANDT
                     PVKPMPSRRRRKGLPKGAQLEWDRHQEEKRNAGDDDFAVSTDYVKRVPKYWDPSDTRGT
                     TVKIAGTTYQKVVDYSGNVHYVEHQEDLLDYVLGKGSYEGLDQDKVLDLTNMLKVDPTE
                     LSSKDKAKARQLAHLLLDLANPVEAVNQLN"
     regulatory      6374..6383
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9893..10573
                     /gene="GP2b"
                     /label="Glycoprotein 2b"
                     /note="Glycoprotein 2b from Equine arteritis virus (strain
                     Bucyrus). Accession#: P28992"
     CDS             10769..11224
                     /gene="GP4"
                     /label="Glycoprotein 4"
                     /note="Glycoprotein 4 from Equine arteritis virus (strain
                     Bucyrus). Accession#: P28994"
     CDS             11215..11979
                     /gene="GP5"
                     /label="Glycoprotein 5"
                     /note="Glycoprotein 5 from Equine arteritis virus (strain
                     Bucyrus). Accession#: P28995"
     CDS             11970..12455
                     /gene="M"
                     /label="Membrane protein"
                     /note="Membrane protein from Equine arteritis virus (strain
                     Bucyrus). Accession#: P28991"
     promoter        12854..12958
                     /label="AmpR promoter"
     CDS             12959..13816
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      13990..14578
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"