LOCUS       V007692                14622 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007692
VERSION     V007692
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 14622)
  AUTHORS   Zhang J, Go YY, Lu Z, Meade BJ, Timoney PJ, Balasuriya UBR.
  TITLE     Development and Characterization of an Infectious cDNA Clone of the
            Modified Live Virus Vaccine Strain of Equine Arteritis Virus and Its
            Potential As a Vaccine Vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14622)
  AUTHORS   Zhang J, Balasuriya UBR.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2010) Veterinary Science, University of Kentucky,
            108 Gluck Equine Research Center, Lexington, KY 40546, USA
REFERENCE   3  (bases 1 to 14622)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14622)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-JUN-2010) Veterinary Science, University of Kentucky, 108 Gluck
            Equine Research Center, Lexington, KY 40546, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             225..9751
                     /codon_start=1
                     /ribosomal_slippage=""
                     /product="replicase polyprotein 1ab"
                     /label="replicase polyprotein 1ab"
                     /note="ORF1ab"
                     /protein_id="AEG80297.1"
                     /translation="MATFSATGFGGSFVRDWSLDLPDACEHGAGLCCEVDGSTLCAECF
                     RGCEGVEQCPGLFMGLLKLASPVPVGHKFLIGWYRAAKVTGRYNFLELLQHPAFAQLRV
                     VDARLAIEEASVFISTDHASAKRFPGARFALTPVYANAWVVSPAANSLIVTTDQEQDGF
                     CWLKLLPPDRREAGLRLYYNHYREQRTGWLSKTGLRLWLGDLGLGINASSGGLKFHIMR
                     GSPQRAWHITTRSCKLKSYYVCDISEADWSCLPAGNYGGYNPPGDGACGYRCLAFMNGA
                     TVVSAGCSSDLWCDDELAYRVFQLSPTFTVTIPGGRVCPNAKYAMICDKQHWRVKRAKG
                     VGLCLDESCFRGTCNCQRMSGPPPAPVSAAVLDHILEAATFGNVRVVIPEGQPRPVPAP
                     RVHPCANSSGDVKDPAPVPPVPKPRTKLAKPNPTQAPIPAPRTRLQGASTQEPLASAGV
                     ASDSAPKWRVAKTVYSSAERFRTELVQRARSVGDVLVQALPHKTPAVQRYTMTLKMMRS
                     RFSWHCDMWYPLAVIACLLPIWPSLALLLSFAIGLIPSVGNNVVLTALLVSSANYVASM
                     DHHCEGAACLALLEEEHYYRAVRWRPITGALSLVLNLLGQVGYVARSTFDAAYVPCTVF
                     DLCSFAILYLCCNRCWRCFGRCVRVGPATHVLGSTGQRVSKLALIDLCDHFSKPTIDVV
                     GMATGWSGCYTGTAAMERQCASTVDPHSFDQKKAGAIVYLTPPVNSGSALQCLNVMWKR
                     PIGSTVLGEQTGAVVTAVKSISFSPPCCVSTTLPTRPGVTVVDHALYNRLTASGVDPAL
                     LRVGQGDFLKLNPGFRLIGGWIYGICYFVLVVVSTFTCLPIKCGIGTRDPFCRRVFSVP
                     VIKTQEHCHAGMCASAEGISLDSLGLTQLQSYWIAAVTSGLVILLVCHRLAISALDLLT
                     LASPLVLLVFPWASVGLLLACSLAGAAVKIQLLATLFVNLFFPQATLVTMGYWACVAAL
                     AVYSLMGLRVKVNVPMCVTPAHFLLLARSAGQSREQMLRVSAAAPTNSLLGVARDCYVT
                     GTTRLYIPKEGGMVFEGLFRSPKARGNVGFVAGSSYGTGSVWTRNNEVVVLTASHVVGR
                     ANMATLKIGDAMLTLTFKKNGDFAEAVTTQSELPGNWPQLHFAQPTTGPASWCTATGDE
                     EGLLSGEVCLAWTTSGDSGSAVVQGDAVVGVHTGSNTSGVAYVTTPSGKLLGADTVTLS
                     SLSKHFTGPLTSIPKDIPDNIIADVDAVPRSLAMLIDGLSNRESSLSGPQLLLIACFMW
                     SYLNQPAYLPYVLGFFAANFFLPKSVGRPVVTGLLWLCCLFTPLSMRLCLFHLVCATVT
                     GNVISLWFYITAAGTSYLSEMWFGGYPTLLFVPRFLVYQFPGWAIGTVLAVCSITMLAA
                     ALGHTLLLDVFSASGRFDRTFMMKYFLEGGVKESVTASVTRAYGKPITQESLTATLAAL
                     TDDDFQFLSDVLDCRAVRSAMNLRAALTSFQVAQYRNILNASLQVDRDAARSRRLMAKL
                     ADFAVEQEVTAGDRVVVIDGLDRMAHFKDDLVLVPLTTKVVGGSRCTICDVVKEEANDT
                     PVKPMPSRRRRKGLPKGAQLEWDRHQEEKRNAGDDDFAVSTDYVKRVPKYWDPSDTRGT
                     TVKIAGTTYQKVVDYSGNVHYVEHQEDLLDYVLGKGSYEGLDQDKVLDLTNMLKVDPTE
                     LSSKDKAKARQLAHLLLDLANPVEAVNQLN*ERPTSFPAMWGVGHLLTLKTRVSWLYTV
                     AQCF*LPGTFYLTSNLCATKSSQRPQKTHCLGTYAPALVTGLFSVVRTGH*LMHTGTVW
                     SAFTRFPPYLILM*AQVTSQ*RVSDGILNLPEEAVQNVSQLIWCTLFKGSTEPLIPMMT
                     RWQLLSVVTRIGRTASCITPVGATFHILSQPMLWKPQLATVLDVRPLPTGPTSSQQLGP
                     SRSNNPYRTSQRACLTTALTSAVTLS*RPLQRQPCVVI*RNTTYPRRVLCCLVFSPWCG
                     RT*KRRLETLHHSTCHLLYHLKIHKPELTALSFLQGLYRATV*LMTWCHSP*KAIYKPP
                     PWRLVNGSTVPNTRLGAFWAPTITLA*VCVPAFRGLRPHSKKLERMGHRFIWASQNSTR
                     YLLLTSTALKQTWRVVIAPPRLWCVGSLLILFLS*LASPSWCTATC*IAVTI*LWQVV*
                     HSPNAGVCHLETLSLPFPIPSIHWCCTPSTCCYVDLKAISQRLQKNILMAAWSCGTCSS
                     TFECTSTRTMWF*PHPTSITRPALTAGSPTCRRC*VSRLTQRKL*TPAPLPFWAAGSSK
                     WTASVIWPVFRTALHALCYTTLVQRIPQSTMKLLFPSLRTPLSAVMKIGGRTSIDVSVA
                     LRVLTELSSPPLKC*HPSAPSSMRVPCAQFVGPPPWPSLLVEGGSVAIVSRTTWVIVTQ
                     PRSSPTAGTTSCTAPLTAQCVRVPQNRWYQKCLTRSWIICCATLITAVKRN*LW*WRMV
                     EQHHRPGATKWVTR*SPWLQTWEATLCLGAVLDHTSQYHFRIRSRAWW*IKL*RTPPPL
                     STWKDPLGVGRLFTWSKMC*PWSVARPWLCPPTRPCWTALTSSNKRAPIHTLWCPSIQF
                     LTFPGLAVETSQCDCHRSELVREKPLWMRWLTSHQWIWRAF*PRVESRVTVI*ISSGAS
                     DPRACHVTFGSDILSAWSPCECAIDSALLCVI*SRAFILIMSQLHIPLKWCLCQIQTLR
                     KV*SSPPTTKIAVLVTAQLIQFKAVHSLL*LFDCPHPNH*RARAQLWRLLGRLRNYTST
                     TPLISLAGC*SSPRKQRRRT*STAHLQHATWAKKLTFGPMRASNITRKSTCCTHTSPSR
                     MV*YTVTLIVALPVAGKSNPTKFRASREWHKIWATTIPQIYQDFAPYRKNSLSIGP*CP
                     MIDTRIACKLPYSKYVNSVNRAQRAIWLDNLFSCRRLV*HLTGLLNGSTAKRVLYQIPY
                     SRPVGSRLTAAFSSMKPRKSLPPLTLMPVWEKLISPPWEDPTSSFPNIYHHCYPQALLP
                     W*VLHWLGKLLKLLAALLMSMLHRLNLIYTLRH*VACTRL*SISSRVGLWCGETRPFMS
                     KRVLMQLHQH*QLCPNSSKCRPMSLFHSMWHQGTEPTRW*RPRLKFQLEPTPPSGHCQL
                     NRHRLVMRSCGDIL*RGSSAQQKCSCAAGVLCLPPQCRPFVH*RDVNLCSTSYKLVQSL
                     GPC"
     CDS             225..5408
                     /codon_start=1
                     /product="replicase polyprotein 1a"
                     /label="replicase polyprotein 1a"
                     /note="ORF1a"
                     /protein_id="AEG80298.1"
                     /translation="MATFSATGFGGSFVRDWSLDLPDACEHGAGLCCEVDGSTLCAECF
                     RGCEGVEQCPGLFMGLLKLASPVPVGHKFLIGWYRAAKVTGRYNFLELLQHPAFAQLRV
                     VDARLAIEEASVFISTDHASAKRFPGARFALTPVYANAWVVSPAANSLIVTTDQEQDGF
                     CWLKLLPPDRREAGLRLYYNHYREQRTGWLSKTGLRLWLGDLGLGINASSGGLKFHIMR
                     GSPQRAWHITTRSCKLKSYYVCDISEADWSCLPAGNYGGYNPPGDGACGYRCLAFMNGA
                     TVVSAGCSSDLWCDDELAYRVFQLSPTFTVTIPGGRVCPNAKYAMICDKQHWRVKRAKG
                     VGLCLDESCFRGTCNCQRMSGPPPAPVSAAVLDHILEAATFGNVRVVIPEGQPRPVPAP
                     RVHPCANSSGDVKDPAPVPPVPKPRTKLAKPNPTQAPIPAPRTRLQGASTQEPLASAGV
                     ASDSAPKWRVAKTVYSSAERFRTELVQRARSVGDVLVQALPHKTPAVQRYTMTLKMMRS
                     RFSWHCDMWYPLAVIACLLPIWPSLALLLSFAIGLIPSVGNNVVLTALLVSSANYVASM
                     DHHCEGAACLALLEEEHYYRAVRWRPITGALSLVLNLLGQVGYVARSTFDAAYVPCTVF
                     DLCSFAILYLCCNRCWRCFGRCVRVGPATHVLGSTGQRVSKLALIDLCDHFSKPTIDVV
                     GMATGWSGCYTGTAAMERQCASTVDPHSFDQKKAGAIVYLTPPVNSGSALQCLNVMWKR
                     PIGSTVLGEQTGAVVTAVKSISFSPPCCVSTTLPTRPGVTVVDHALYNRLTASGVDPAL
                     LRVGQGDFLKLNPGFRLIGGWIYGICYFVLVVVSTFTCLPIKCGIGTRDPFCRRVFSVP
                     VIKTQEHCHAGMCASAEGISLDSLGLTQLQSYWIAAVTSGLVILLVCHRLAISALDLLT
                     LASPLVLLVFPWASVGLLLACSLAGAAVKIQLLATLFVNLFFPQATLVTMGYWACVAAL
                     AVYSLMGLRVKVNVPMCVTPAHFLLLARSAGQSREQMLRVSAAAPTNSLLGVARDCYVT
                     GTTRLYIPKEGGMVFEGLFRSPKARGNVGFVAGSSYGTGSVWTRNNEVVVLTASHVVGR
                     ANMATLKIGDAMLTLTFKKNGDFAEAVTTQSELPGNWPQLHFAQPTTGPASWCTATGDE
                     EGLLSGEVCLAWTTSGDSGSAVVQGDAVVGVHTGSNTSGVAYVTTPSGKLLGADTVTLS
                     SLSKHFTGPLTSIPKDIPDNIIADVDAVPRSLAMLIDGLSNRESSLSGPQLLLIACFMW
                     SYLNQPAYLPYVLGFFAANFFLPKSVGRPVVTGLLWLCCLFTPLSMRLCLFHLVCATVT
                     GNVISLWFYITAAGTSYLSEMWFGGYPTLLFVPRFLVYQFPGWAIGTVLAVCSITMLAA
                     ALGHTLLLDVFSASGRFDRTFMMKYFLEGGVKESVTASVTRAYGKPITQESLTATLAAL
                     TDDDFQFLSDVLDCRAVRSAMNLRAALTSFQVAQYRNILNASLQVDRDAARSRRLMAKL
                     ADFAVEQEVTAGDRVVVIDGLDRMAHFKDDLVLVPLTTKVVGGSRCTICDVVKEEANDT
                     PVKPMPSRRRRKGLPKGAQLEWDRHQEEKRNAGDDDFAVSTDYVKRVPKYWDPSDTRGT
                     TVKIAGTTYQKVVDYSGNVHYVEHQEDLLDYVLGKGSYEGLDQDKVLDLTNMLKVDPTE
                     LSSKDKAKARQLAHLLLDLANPVEAVNQLN"
     regulatory      6374..6383
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9893..10573
                     /gene="GP2b"
                     /label="Glycoprotein 2b"
                     /note="Glycoprotein 2b from Equine arteritis virus (strain
                     Bucyrus). Accession#: P28992"
     CDS             10769..11224
                     /gene="GP4"
                     /label="Glycoprotein 4"
                     /note="Glycoprotein 4 from Equine arteritis virus (strain
                     Bucyrus). Accession#: P28994"
     CDS             11215..11979
                     /gene="GP5"
                     /label="Glycoprotein 5"
                     /note="Glycoprotein 5 from Equine arteritis virus (strain
                     Bucyrus). Accession#: P28995"
     CDS             11970..12455
                     /gene="M"
                     /label="Membrane protein"
                     /note="Membrane protein from Equine arteritis virus (strain
                     Bucyrus). Accession#: P28991"
     promoter        12854..12958
                     /label="AmpR promoter"
     CDS             12959..13816
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      13990..14578
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gctcgaagtg tgtatggtgc catatacggc tcaccgccat atgcactgca agaattacta
       61 ttcttgtggg cccctctcgg taaatcctag agggctttcc tctcgttatt gcgagattcg
      121 tcgttagata acggcaagtt ccctttctta ctatcctatt ttcatcttgt ggcttgacgg
      181 gtcactgcca tcgtcgtcga tctctatcaa ctacccttgc gactatggca accttctccg
      241 ctactggatt tggagggagt tttgttaggg actggtccct ggacttaccc gacgcttgtg
      301 agcatggcgc gggattgtgc tgtgaagtgg acggctccac cttatgcgcc gagtgttttc
      361 gcggttgcga aggagtggag caatgtcctg gcttgttcat gggactgtta aaactggctt
      421 cgccagttcc agtgggacat aagttcctga ttggttggta tcgagctgcc aaagtcaccg
      481 ggcgttacaa tttccttgag ctgttgcaac accctgcttt cgcccagctg cgtgtggttg
      541 atgctaggtt agccattgaa gaggcaagtg tgtttatttc cactgaccac gcgtctgcta
      601 agcgtttccc tggcgctaga tttgcgctga caccggtgta tgctaacgct tgggttgtga
      661 gcccggctgc taacagtttg atagtgacca ctgaccagga acaagatggg ttctgctggt
      721 taaaactttt accacctgac cgccgtgagg ctggtttgcg gttgtattac aaccattacc
      781 gcgaacaaag gaccgggtgg ctgtctaaaa caggacttcg cttatggctt ggagacctgg
      841 gtttgggcat caatgcgagc tctggagggc tgaaattcca cattatgagg ggttcgcctc
      901 agcgagcttg gcatatcaca acacgcagct gcaagctgaa gagctactac gtttgtgaca
      961 tctctgaagc agactggtcc tgtttgcccg ctggcaacta cggcggctac aatccaccag
     1021 gggacggagc ttgcggttac aggtgcttgg ccttcatgaa tggcgccact gttgtgtcgg
     1081 ctggttgcag ttctgacttg tggtgtgatg atgagttggc ttatcgagtc tttcaattgt
     1141 cacccacgtt cacggttacc atcccaggtg ggcgagtttg tccgaatgcc aagtacgcaa
     1201 tgatttgtga caagcagcac tggcgcgtca aacgtgcaaa gggcgtcggc ctgtgtctcg
     1261 atgaaagctg tttcaggggc acctgcaatt gccaacgcat gagtggacca ccacctgcac
     1321 ccgtgtcagc cgccgtgtta gatcacatac tggaggcggc gacgtttggc aacgttcgcg
     1381 tggttatacc tgaagggcag ccacgccccg taccagcgcc gcgagttcat ccctgcgcca
     1441 actcttctgg agatgtcaaa gatccggcgc ccgttccgcc agtaccaaaa ccaaggacca
     1501 agcttgccaa accgaaccca actcaggcgc ccatcccagc accgcgcacg cgacttcaag
     1561 gggcctcaac acaggagcca ctggcgagtg cgggagttgc ttctgactcg gcacccaaat
     1621 ggcgtgtggc caaaactgtg tacagctccg cggagcgctt tcggaccgaa ctggtacaac
     1681 gtgctcggtc cgttggggac gttcttgttc aagcgctacc gcacaaaacc ccagcagtgc
     1741 agcggtatac catgactctg aagatgatgc gttcacgctt cagttggcac tgcgacatgt
     1801 ggtacccttt ggctgtaatc gcttgtttgc tccccatatg gccatctctt gctttgctcc
     1861 ttagcttcgc cattgggttg atacccagtg tgggcaataa tgttgttctg acagcgcttc
     1921 tggtttcatc agctaattat gttgcgtcaa tggaccatca ctgtgaaggt gcggcttgct
     1981 tagccttgct ggaagaagaa cactattata gagcggtccg ttggcgcccg attacaggcg
     2041 cgctgtcgct tgtgctcaat ttactggggc aggtaggcta tgtagctcgt tccacctttg
     2101 atgcagctta tgttccttgc actgtgttcg atctttgcag ctttgctatt ctgtacctct
     2161 gctgcaatcg ttgctggaga tgcttcggac gctgtgtgcg agttgggcct gccacgcatg
     2221 ttttgggttc caccgggcaa cgagtttcca aactggcgct cattgatttg tgtgaccact
     2281 tttcaaagcc caccatcgac gttgtgggca tggcaactgg ttggagcgga tgttacacag
     2341 gaaccgccgc aatggagcgt cagtgtgcct ctacggtgga ccctcactcg ttcgaccaga
     2401 agaaggcagg agcgattgtt tacctcaccc cccctgtcaa cagcgggtct gcgctgcagt
     2461 gcctcaatgt catgtggaag cgaccaattg ggtccactgt ccttggggaa caaacaggag
     2521 ctgttgtgac ggcggtcaag agcatctctt tctcacctcc ctgctgcgtc tctaccactt
     2581 tgcccacccg acccggtgtg accgttgtcg accatgctct ttacaaccgg ttgactgctt
     2641 caggggtcga tcccgcttta ttgcgtgttg ggcaaggtga ttttctaaaa cttaatccgg
     2701 ggttccggct gataggtgga tggatttatg ggatatgcta ttttgtgttg gtggttgtgt
     2761 caacttttac ctgcctacct atcaaatgtg gcattggcac ccgcgaccct ttctgccgca
     2821 gagtgttttc tgtacccgtc atcaagaccc aagagcactg ccatgctgga atgtgtgcta
     2881 gcgctgaagg catctctctg gactctctgg ggttaactca gttacaaagt tattggatcg
     2941 cagccgtcac tagcggatta gtgatcttgt tggtctgcca ccgcctggcc atcagcgcct
     3001 tggacttgtt gactctagct tcccctttag tgttgcttgt gttcccttgg gcatctgtgg
     3061 ggcttttact tgcttgcagt ctcgctggtg ctgctgtgaa aatacagttg ttggcgacgc
     3121 tttttgtgaa tctgttcttt ccccaagcta cccttgtcac tatgggatac tgggcgtgcg
     3181 tggcggcttt ggccgtttac agtttgatgg gcttgcgagt gaaagtgaat gtgcccatgt
     3241 gtgtgacacc tgcccatttt ctgctgctgg cgaggtcagc tggacagtca agagagcaga
     3301 tgctccgggt cagcgctgct gcccccacca attcactgct tggagtggct cgtgattgtt
     3361 atgtcacagg cacaactcgg ctgtatatac ccaaggaagg cgggatggtg tttgaagggc
     3421 tattcaggtc accgaaggcg cgcggcaacg tcggcttcgt ggctggtagc agctacggca
     3481 cagggtcagt gtggaccagg aacaacgagg tcgtcgtact gacagcgtca cacgtggttg
     3541 gccgcgctaa catggccact ctgaagatcg gtgacgcaat gctgactctg actttcaaaa
     3601 aaaatggcga cttcgccgag gcagtgacga cacagtccga gctcccaggc aattggccac
     3661 agttgcattt cgcccaacca acaaccgggc ccgcttcatg gtgcactgcc acaggagatg
     3721 aagaaggctt gctcagtggc gaggtttgtc tggcgtggac tactagtggc gactctggat
     3781 cagcagtggt tcagggtgac gctgtggtag gggtccacac cggttcgaac acaagtggtg
     3841 ttgcctacgt gaccacccca agcggaaaac tccttggcgc cgacaccgtg actttgtcgt
     3901 cactgtcaaa gcatttcaca ggccctttga catcaatccc gaaggacatc cctgacaaca
     3961 tcattgccga tgttgatgct gttcctcgtt ctctggccat gctgattgat ggcttatcta
     4021 atagagagag cagcctttct ggacctcagt tgttgttaat tgcttgtttt atgtggtctt
     4081 atcttaacca acctgcctac ttgccttatg tgctgggctt ctttgccgct aacttcttcc
     4141 tgccaaaaag tgttggccgc cctgtggtca ctgggcttct atggttgtgc tgcctcttca
     4201 caccgctttc catgcgcttg tgcttgttcc atctggtctg tgctaccgtc acgggaaacg
     4261 tgatatcttt gtggttctac atcactgccg ctggcacgtc ttacctttct gagatgtggt
     4321 tcggaggcta tcccaccttg ttgtttgtgc cacggttcct agtgtaccag ttccccggct
     4381 gggctattgg cacagtacta gcggtatgca gcatcaccat gctggctgct gccctcggtc
     4441 acaccctgtt actggatgtg ttctccgcct cgggtcgctt tgacaggact ttcatgatga
     4501 aatacttcct ggagggagga gtgaaagaga gtgtcaccgc ctcagtcacc cgcgcttatg
     4561 gcaaaccaat tacccaggag agtctcactg caacattagc tgccctcact gatgatgact
     4621 tccaattcct ctctgatgtg cttgactgtc gggccgtccg atcggcaatg aatctgcgtg
     4681 ccgctctcac aagttttcaa gtggcgcagt atcgtaacat ccttaatgca tccttgcaag
     4741 tcgatcgtga cgctgctcgt agtcgcagac taatggcaaa actggctgat tttgcggttg
     4801 aacaagaagt aacagccgga gaccgtgttg ttgttatcga cggtctggac cgcatggctc
     4861 acttcaaaga cgatttggtg ctggttcctt tgaccaccaa agtagtaggc ggttctaggt
     4921 gcaccatttg tgacgtcgtt aaggaagaag ccaatgacac cccagttaag ccaatgccca
     4981 gcaggagacg ccgcaagggc ctgcctaaag gtgctcagtt ggagtgggac cgtcaccagg
     5041 aagagaagag gaacgccggt gatgatgatt ttgcggtctc gactgattat gtcaagagag
     5101 tgccaaagta ctgggacccc agcgacaccc gaggcacgac agtgaaaatc gccggcacta
     5161 cctatcagaa agtggttgac tattcaggca atgtgcatta cgtggagcat caggaagatc
     5221 tgctagacta cgtgctgggc aaggggagct atgaaggcct agatcaggac aaagtgttgg
     5281 acctcacaaa catgcttaaa gtggacccca cggagctctc ctccaaagac aaagccaagg
     5341 cgcgtcagct tgctcatctg ctgttggatc tggctaaccc agttgaggca gtgaatcagt
     5401 taaactgaga gcgccccaca tctttcccgg cgatgtgggg cgtcggacat ttgctgactc
     5461 taaagacaag ggtttcgtgg ctctacacag tcgcacaatg tttttagctg cccgggactt
     5521 tttatttaac atcaaatttg tgtgcgacga agagttcaca aagaccccaa aagacacact
     5581 gcttgggtac gtacgcgcct gccctggtta ctggtttatt ttccgtcgta cgcaccggtc
     5641 actgattgat gcatactggg acagtatgga gtgcgtttac gcgcttccca ccatatctga
     5701 ttttgatgtg agcccaggtg acgtcgcagt gacgggtgag cgatgggatt ttgaatctcc
     5761 cggaggaggc cgtgcaaaac gtctcacagc tgatctggtg cacgcttttc aagggttcca
     5821 cggagcctct tattcctatg atgacaaggt ggcagctgct gtcagtggtg acccgtatcg
     5881 gtcggacggc gtcttgtata acacccgttg gggcaacatt ccatattctg tcccaaccaa
     5941 tgctttggaa gccacagctt gctaccgtgc tggatgtgag gccgttaccg acgggaccaa
     6001 cgtcatcgca acaattgggc ccttcccgga gcaacaaccc ataccggaca tcccaaagag
     6061 cgtgcttgac aactgcgctg acatcagctg tgacgctttc atagcgcccg ctgcagagac
     6121 agccctgtgt ggtgatttag agaaatacaa cctatccacg cagggttttg tgttgcctag
     6181 tgttttctcc atggtgcggg cgtacttaaa agaggagatt ggagacgctc caccactcta
     6241 cttgccatct actgtaccat ctaaaaattc acaagccgga attaacggcg ctgagtttcc
     6301 tacaaggtct ttacagagct actgtttgat tgatgacatg gtgtcacagt ccatgaaaag
     6361 caatctacaa accgccacca tggcgacttg taaacggcag tactgttcca aatacaagat
     6421 taggagcatt ctgggcacca acaattacat tggcctaggt ttgcgtgcct gcctttcggg
     6481 ggttacggcc gcattccaaa aagctggaaa ggatgggtca ccgatttatt tgggcaagtc
     6541 aaaattcgac ccgatacctg ctcctgacaa gtactgcctt gaaacagacc tggagagttg
     6601 tgatcgctcc accccggctt tggtgcgttg gttcgctact aatcttattt ttgagctagc
     6661 tggccagccc gagttggtgc acagctacgt gttgaattgc tgtcacgatc tagttgtggc
     6721 aggtagtgta gcattcacca aacgcggggg tttgtcatct ggagacccta tcacttccat
     6781 ttccaatacc atctattcat tggtgctgta cacccagcac atgttgctat gtggacttga
     6841 aggctatttc ccagagattg cagaaaaata tcttgatggc agcctggagc tgcgggacat
     6901 gttcaagtac gttcgagtgt acatctactc ggacgatgtg gttctaacca cacccaacca
     6961 gcattacgcg gccagctttg accgctgggt cccccacctg caggcgctgc taggtttcaa
     7021 ggttgaccca aagaaaactg tgaacaccag ctccccttcc tttttgggct gccggttcaa
     7081 gcaagtggac ggcaagtgtt atctggccag tcttcaggac cgcgttacac gctctctgtt
     7141 ataccacatt ggtgcaaaga atccctcaga gtactatgaa gctgctgttt ccatctttaa
     7201 ggactccatt atctgctgtg atgaagattg gtggacggac ctccatcgac gtatcagtgg
     7261 cgctgcgcgt actgacggag ttgagttccc caccattgaa atgttaacat ccttccgcac
     7321 caagcagtat gagagtgccg tgtgcacagt ttgtggggcc gcccccgtgg ccaagtctgc
     7381 ttgtggaggg tggttctgtg gcaattgtgt cccgtaccac gtgggtcatt gtcacacaac
     7441 ctcgctcttc gccaactgcg ggcacgacat catgtaccgc tccacttact gcacaatgtg
     7501 tgagggttcc ccaaaacaga tggtaccaaa agtgcctcac ccgatcctgg atcatttgct
     7561 gtgccacgtt gattacggca gtaaagagga actaactctg gtagtggcgg atggtcgaac
     7621 aacatcaccg cccgggcgct acaaagtggg tcacaaggta gtcgccgtgg ttgcagacgt
     7681 gggaggcaac attgtgtttg ggtgcggtcc tggatcacac atcgcagtac cacttcagga
     7741 tacgctcaag ggcgtggtgg tgaataaagc tctgaagaac gccgccgcct ctgagtacgt
     7801 ggaaggaccc cctgggagtg ggaagacttt tcacctggtc aaagatgtgc tagccgtggt
     7861 cggtagcgcg accttggttg tgcccaccca cgcgtccatg ctggactgca ttaacaagct
     7921 caaacaagcg ggcgccgatc catactttgt ggtgcccaag tatacagttc ttgactttcc
     7981 ccggcctggc agtggaaaca tcacagtgcg actgccacag gtcggaacta gtgagggaga
     8041 aacctttgtg gatgaggtgg cttacttctc accagtggat ctggcgcgca ttttaaccca
     8101 gggtcgagtc aagggttacg gtgatttaaa tcagctcggg tgcgtcggac ccgcgagcgt
     8161 gccacgtaac ctttggctcc gacattttgt cagcctggag cccttgcgag tgtgccatcg
     8221 attcggcgct gctgtgtgtg atttgatcaa gggcatttat ccttattatg agccagctcc
     8281 acataccact aaagtggtgt ttgtgccaaa tccagacttt gagaaaggtg tagtcatcac
     8341 cgcctaccac aaagatcgcg gtcttggtca ccgcacaatt gattcaattc aaggctgtac
     8401 attccctgtt gtgactcttc gactgcccac accccaatca ctgacgcgcc cgcgcgcagt
     8461 tgtggcggtt actagggcgt ctcaggaatt atacatctac gacccctttg atcagcttag
     8521 cgggttgttg aagttcacca aggaagcaga ggcgcaggac ttgatccacg gcccacctac
     8581 agcatgccac ctgggccaag aaattgacct ttggtccaat gagggcctcg aatattacaa
     8641 ggaagtcaac ctgctgtaca cacacgtccc catcaaggat ggtgtaatac acagttaccc
     8701 taattgtggc cctgcctgtg gctgggaaaa gcaatccaac aaaatttcgt gcctcccgag
     8761 agtggcacaa aatttgggct accactattc cccagattta ccaggatttt gccccatacc
     8821 gaaagaactc gctgagcatt ggcccgtagt gtccaatgat agatacccga attgcttgca
     8881 aattacctta cagcaagtat gtgaactcag taaaccgtgc tcagcgggct atatggttgg
     8941 acaatctgtt ttcgtgcaga cgcctggtgt gacatcttac tggcttactg aatgggtcga
     9001 cggcaaagcg cgtgctctac cagattcctt attctcgtcc ggtaggttcg agactaacag
     9061 ccgcgttttc ctcgatgaag ccgaggaaaa gtttgccgcc gctcaccctc atgcctgttt
     9121 gggagaaatt aataagtcca ccgtgggagg atcccacttc atcttttccc aatatttacc
     9181 accactgcta cccgcaggcg ctgttgccct ggtaggtgct tcattggctg ggaaagctgc
     9241 taaagctgct tgcagcgttg ttgatgtcta tgctccatcg tttgaacctt atctacaccc
     9301 tgagacactg agtcgcgtgt acaagattat gatcgatttc aagccgtgta ggcttatggt
     9361 gtggagaaac gcgacctttt atgtccaaga gggtgttgat gcagttacat cagcactagc
     9421 agctgtgtcc aaactcatca aagtgccggc caatgagcct gtttcattcc atgtggcatc
     9481 agggtacaga accaacgcgc tggtagcgcc ccaggctaaa atttcaattg gagcctacgc
     9541 cgccgagtgg gcactgtcaa ctgaaccgcc accggctggt tatgcgatcg tgcggcgata
     9601 tattgtaaag aggctcctca gctcaacaga agtgttcttg tgccgcaggg gtgttgtgtc
     9661 ttccacctca gtgcagacca tttgtgcact agagggatgt aaacctctgt tcaacttctt
     9721 acaaattggt tcagtcattg ggcccgtgtg aggcgcgccc aaccttaagg tggcctacgg
     9781 cagcaaagtc aaccttgtga ggttgggggc agctgaggta tgggcttagt gtggtcactg
     9841 atttcaaatt ctattcagac tattattgct gattttgcta tttctgtgat tgatgcagcg
     9901 cttttctttc tcatgctact tgcattggct gttgttactg tgtttctttt ctggctcatt
     9961 gttgccatcg gccgcagctt ggtggcgcgg tgttcacgag gtgcgcgtta cagacctgtt
    10021 taaggatttg cagtgcgaca acctgcgcgc gaaagatgcc ttcccgagtc tgggacatgc
    10081 tctgtcgatt ggccagtcga ggctatcgta tatgctgcag gattggttgc ttgctgcgca
    10141 ccgcaaggaa gttatgcctt ccaataccat gcctatgccc ggtcttactc ctgattgctt
    10201 tgaccatctg gagtcttcta gctatgctcc atttatcaat gcctatcggc aggcaatttt
    10261 gagtcaatac tcacaagagc tcctgctcga agccatcaac tgtaaattgc ttgctgtggt
    10321 tgcaccggca ttgtatcaca attaccatct agccaatttg accgaaccgg ccacatgggt
    10381 cgtgcctaca gtgggccagt tgcactatta tgcttcttcc tctatttttg cttcatctgt
    10441 ggaagtgttg gcagcaataa tactactatt tgcatgcata ccactagtga cacgagtgta
    10501 catctctttt acgcggctaa tgtcaccttc ccgtcgcact tccagcggca ctttgccgcc
    10561 gcgcaagatt ttgtagtgca cacgggttat gaatatgccg gggtcactat ggtagtgcac
    10621 ttgtttgcca acttggttct gacatttccg agcttagtta attgttcccg ccctgtgaat
    10681 gtctttgcta atgcttcttg cgtgcaagtg gtttgtagtc ataccaactc aactactggc
    10741 tcgggtcaac tttcgttttc ctttgtagat gaagatctcc ggctgcatat caggcctact
    10801 cttatttgtt ggtttgcctt gttgttggtg cactttctac ccatgccacg ctacagaggc
    10861 tcgcaatttt acttacacta gtcatggatt gggccacgtg cacggtcatg aggggtgtag
    10921 gaattttatt aatgtcactc attctgcatt tctttatctt aatcccacca ctctcactgc
    10981 gccggctata actcattgtt tacttctggt tctggcagcc aaaatggaac acccaaacgc
    11041 tactatctgg ctgcagctgc agccgtttgg gtatcatgtg gctggcgatg tcattgtcaa
    11101 cttggaagag aataagaggc atccttactt taaactcttg agagcgccgg ctttaccgct
    11161 tggttttgtg gctatagttt atgttctttt acgactggta cgttgggctc aacaatgcta
    11221 tctatgattg tattgttatt cttgctttgg ggtgcgccat cacatgctta cttctcatac
    11281 tacaccgctc agcgcttcac agacttcacc ttgtgcatgc tgacggatcg cggcgttatt
    11341 gccaatttgc tgcgatatga tgagcacact gctttgtaca attgttccgc cagtaaaacc
    11401 tgttggtatt gcacattccc ggacgaaaag attatcacgt ttggaaccga ttgtgatgac
    11461 acctacgcgg tcccagttgc tgaggtcctg gaacaggcgc atggaccgta cggtgtgctg
    11521 tttggtgacg tgcccccttt tatttactat ggccgtgaat tcggcatagt tgtgttggat
    11581 gtgtttatgt tctatcccgt tttagttctg tttttcttat cagtactacc ctatgctacg
    11641 cttattcttg aaatgtgtgt atctattctg tttataatct atggcattta cagcggggcc
    11701 tacttggcca tgggcatatt ttcggccacg cttgctatac attcaattgt ggtcctccgc
    11761 caattactgt ggttatgcct ggcttggcga taccgctgca cgcttcacgc gtcctttata
    11821 tcagctgagg ggaaagtgta ccccgtagac cccgaactcc cggttgccgc cgcgggcaat
    11881 cggttgctag tcccaggtag gcccactatc gattatgcag tggcctacgg cagcaaagtc
    11941 aaccttgtga ggttgggggc agctgaggta tgggagccat agattcattt tgtggtgacg
    12001 ggattttagg tgagtatcta gattacttta ttctgtccgt cccactcttg ctgttgctta
    12061 ctaggtatgt agcatctggg tcagtgtatg ttttgactgc cttgttctat tccttagtat
    12121 tagcagctta tatttggttt gtcatagttg gaagagcctt ttccactgct tatgcttttg
    12181 cgcttttggc tgcttttctg ttattagtaa cgaggatgat tgtaggtatg atgcctcgtc
    12241 ttcggtccat tttcaaccat cgccaactgg tggtagctga ttttgtggac acacctagtg
    12301 gacctgttcc catcccccgc tcaactactc aggtagtggt tcgcggcaac gggtacaccg
    12361 cagttggtaa caagcttgtc gatggcgtca agacgatcac gtccgcaggc cgcctctgtt
    12421 cgaaacggat ggcggcgaca gcctacaagc tacaatgacc tactgcgcat gtttggtcag
    12481 atgcgggtcc ggaaaccgcc cgcgcaaccc actcaggcta ttattgcaga gcctggagac
    12541 cttaggcatg atttaaatca acaggagcgc gccacccttt cgtcgaacgt acaacggttc
    12601 ttcatgattg ggcatggttc actcactgca gatgccggag gactcacgta caccgtcagt
    12661 tgggttccta ccaaacaaat ccagcgcaaa attgcgcctc cagcagggcc gtaagacgtg
    12721 gatattctcc tgtgtggcgt catgttgaag tagttattag ccacccagga accaaaaaaa
    12781 aaaaaaaaaa aaactcgagg ggaattaatt cttgaagacg aaagggccag gtggcacttt
    12841 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
    12901 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
    12961 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
    13021 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
    13081 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
    13141 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
    13201 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
    13261 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
    13321 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
    13381 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
    13441 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
    13501 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
    13561 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
    13621 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
    13681 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
    13741 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
    13801 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
    13861 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
    13921 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    13981 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
    14041 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
    14101 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
    14161 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
    14221 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
    14281 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    14341 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    14401 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    14461 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    14521 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    14581 cgccagcaac gcgagctctc tagattaata cgactcacta ta
//
