pDSG262 vector (V007920)

LOCUS       40924_15205        3560 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pDSG262, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Glass DS, Riedel-Kruse IH.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming 
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Glass DS.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318 
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
REFERENCE   3  (bases 1 to 3560)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3560)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell (2018)
            In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-JUN-2018) Bioengineering, Stanford University, 318 Campus Drive,
            Clark Center E350, Stanford, CA 94305, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2..61
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(134..156)
                     /label=VR primer site
                     /note="VR primer site"
     misc_feature    complement(139..156)
                     /label=VR primer site
                     /note="VR primer site"
     CDS             complement(222..1034)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(1629..2173)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     misc_feature    2591..2611
                     /label=VF2 primer site
                     /note="VF2 primer site"
     terminator      complement(2663..2706)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2709..2730
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     misc_feature    2731..2779
                     /label=pLambda (BBa_R0051)
                     /note="pLambda (BBa_R0051)"
     misc_feature    2788..2799
                     /label=RBS (BBa_B0034)
                     /note="RBS (BBa_B0034)"
     RBS             2788..2799
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2806..3516
                     /label=mRuby2
                     /note="monomeric red fluorescent protein derived from
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
     CDS             3517..3534
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_feature    3541..3560
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"