LOCUS       40924_15205        3560 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pDSG262, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Glass DS, Riedel-Kruse IH.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming 
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Glass DS.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318 
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
REFERENCE   3  (bases 1 to 3560)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3560)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell (2018)
            In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-JUN-2018) Bioengineering, Stanford University, 318 Campus Drive,
            Clark Center E350, Stanford, CA 94305, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2..61
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(134..156)
                     /label=VR primer site
                     /note="VR primer site"
     misc_feature    complement(139..156)
                     /label=VR primer site
                     /note="VR primer site"
     CDS             complement(222..1034)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(1629..2173)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     misc_feature    2591..2611
                     /label=VF2 primer site
                     /note="VF2 primer site"
     terminator      complement(2663..2706)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2709..2730
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     misc_feature    2731..2779
                     /label=pLambda (BBa_R0051)
                     /note="pLambda (BBa_R0051)"
     misc_feature    2788..2799
                     /label=RBS (BBa_B0034)
                     /note="RBS (BBa_B0034)"
     RBS             2788..2799
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2806..3516
                     /label=mRuby2
                     /note="monomeric red fluorescent protein derived from
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
     CDS             3517..3534
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_feature    3541..3560
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 gtccggcaaa aaaacgggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag
       61 attacttcgc gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      121 gcggcgagcg gtatcagctc actcaaaggc ggtaatctcg agtcccgtca agtcagcgta
      181 atgctctgcc agtgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc
      241 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
      301 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
      361 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
      421 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
      481 agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
      541 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
      601 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
      661 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
      721 gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
      781 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
      841 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
      901 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
      961 ccatataaat cagcatccat gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt
     1021 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
     1081 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg
     1141 ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agatcacgca tcttcccgac
     1201 aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca ccaactggtc cacctacaac
     1261 aaagctctca tcaaccgtgg ctccctcact ttctggctgg atgatggggc gattcaggcc
     1321 tggtatgagt cagcaacacc ttcttcacga ggcagacctc agcgctagcg gagtgtatac
     1381 tggcttacta tgttggcact gatgagggtg tcagtgaagt gcttcatgtg gcaggagaaa
     1441 aaaggctgca ccggtgcgtc agcagaatat gtgatacagg atatattccg cttcctcgct
     1501 cactgactcg ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt acgaacgggg
     1561 cggagatttc ctggaagatg ccaggaagat acttaacagg gaagtgagag ggccgcggca
     1621 aagccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg acgctcaaat
     1681 cagtggtggc gaaacccgac aggactataa agataccagg cgtttcccct ggcggctccc
     1741 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     1801 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     1861 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
     1921 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
     1981 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
     2041 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
     2101 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
     2161 aaaacgatct caagaagatc atcttattaa ggggtctgac gctcagtgga acgaaaactc
     2221 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2281 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     2341 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     2401 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     2461 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     2521 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     2581 tattccatgg tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     2641 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat
     2701 gatttctgga attcgcggcc gcttctagag taacaccgtg cgtgttgact attttacctc
     2761 tggcggtgat aatggttgct actagagaaa gaggagaaat actagatggt gtctaagggc
     2821 gaagagctga tcaaggaaaa tatgcgtatg aaggtggtca tggaaggttc ggtcaacggc
     2881 caccaattca aatgcacagg tgaaggagaa ggcaatccgt acatgggaac tcaaaccatg
     2941 aggatcaaag tcatcgaggg aggacccctg ccatttgcct ttgacattct tgccacgtcg
     3001 ttcatgtatg gcagccgtac ttttatcaag tacccgaaag gcattcctga tttctttaaa
     3061 cagtcctttc ctgagggttt tacttgggaa agagttacga gatacgaaga tggtggagtc
     3121 gtcaccgtca tgcaggacac cagccttgag gatggctgtc tcgtttacca cgtccaagtc
     3181 agaggggtaa actttccctc caatggtccc gtgatgcaga agaagaccaa gggttgggag
     3241 cctaatacag agatgatgta tccagcagat ggtggtctga ggggatacac tcatatggca
     3301 ctgaaagttg atggtggtgg ccatctgtct tgctctttcg taacaactta caggtcaaaa
     3361 aagaccgtcg ggaacatcaa gatgcccggt atccatgccg ttgatcaccg cctggaaagg
     3421 ttagaggaaa gtgacaatga aatgttcgta gtacaacgcg aacacgcagt tgccaagttc
     3481 gccgggcttg gtggtgggat ggacgagctg tacaagcatc accaccacca tcactaatga
     3541 tactagtagc ggccgctgca
//
