pDO2 vector (V007961)

LOCUS       V007961                 9386 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007961
VERSION     V007961
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9386)
  AUTHORS   Ahmed A.
  TITLE     Double origin vectors for isolating bidirectional deletions for DNA
            sequence analysis
  JOURNAL   Gene In press
REFERENCE   2  (bases 1 to 9386)
  AUTHORS   Ahmed A.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-1993) Ahmed A., University of Alberta, Genetics,
            Biological Sciences Bldg Rm G502, Edmonton, Alberta, T6G 2E1, Canada
REFERENCE   3  (bases 1 to 9386)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9386)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene In
            press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-NOV-1993) Ahmed A., University of Alberta, Genetics, Biological
            Sciences Bldg Rm G502, Edmonton, Alberta, T6G 2E1, Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1168..1959)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     RBS             2756..2764
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             2768..2965
                     /gene="cro"
                     /label="Regulatory protein cro"
                     /note="Regulatory protein cro from Escherichia phage
                     lambda. Accession#: P03040"
     terminator      complement(3315..3409)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     primer_bind     5981..5997
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(6094..6110)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6118..6134)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6142..6172)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6187..6208)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6496..7084)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7258..8115)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8116..8220)
                     /label="AmpR promoter"
     misc_feature    9203..9386
                     /label="cos"
                     /note="lambda cos site; allows packaging into phage lambda
                     particles"