LOCUS       V007961                 9386 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007961
VERSION     V007961
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9386)
  AUTHORS   Ahmed A.
  TITLE     Double origin vectors for isolating bidirectional deletions for DNA
            sequence analysis
  JOURNAL   Gene In press
REFERENCE   2  (bases 1 to 9386)
  AUTHORS   Ahmed A.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-1993) Ahmed A., University of Alberta, Genetics,
            Biological Sciences Bldg Rm G502, Edmonton, Alberta, T6G 2E1, Canada
REFERENCE   3  (bases 1 to 9386)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9386)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene In
            press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-NOV-1993) Ahmed A., University of Alberta, Genetics, Biological
            Sciences Bldg Rm G502, Edmonton, Alberta, T6G 2E1, Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1168..1959)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     RBS             2756..2764
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             2768..2965
                     /gene="cro"
                     /label="Regulatory protein cro"
                     /note="Regulatory protein cro from Escherichia phage
                     lambda. Accession#: P03040"
     terminator      complement(3315..3409)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     primer_bind     5981..5997
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(6094..6110)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6118..6134)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6142..6172)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6187..6208)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6496..7084)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7258..8115)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8116..8220)
                     /label="AmpR promoter"
     misc_feature    9203..9386
                     /label="cos"
                     /note="lambda cos site; allows packaging into phage lambda
                     particles"
ORIGIN
        1 aggtcgccgc cccgtaacct gtcggatcac cggaaaggac ccgtaaagtg ataatgatta
       61 tcatctacat atcacaacgt gcgtggaggc catcaaacca cgtcaaataa tcaattatga
      121 cgcaggtatc gtattaattg atctgcatca acttaacgta aaaacaactt cagacaatac
      181 aaatcagcga cactgaatac ggggcaacct catgtcaacg aagaacagaa cccgcagaac
      241 aacaacccgc aacatccgct ttcctaacca aatgattgaa caaattaaca tcgctcttga
      301 gcaaaaaggg tccgggaatt tctcagcctg ggtcattgaa gcctgccgtc ggagactaac
      361 gtcagaaaag agagcatata catcaattaa aagtgatgaa gaatgaacat cccgcgttct
      421 tccctccgaa caggacgata ttgtaaattc acttaattac gagggcattg cagtaattgg
      481 gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc tccagtggct
      541 tctgtttcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg taacggcaaa
      601 agcaccgccg gacatcagcg ctatctctgc tctcactgcc gtaaaacatg gcaactgcag
      661 ttcacttaca ccgcttctca acccggtacg caccagaaaa tcattgatat ggccatgaat
      721 ggcgttggat gccgggcaac agcccgcatt atgggcgttg gcctcaacac gattttacgt
      781 cacttaaaaa actcaggccg cagtcggtaa ccggcctctt catcgggaat gcgcgcgacc
      841 ttcagcatcg ccggcatgtc cccctggcgg acgggaagta tccagctcga gtgggtggtg
      901 agcagctcgc cgtcgcgcag caggcgccag cgctccatcc acgcagtgac ctcaggcagc
      961 agctgaacca actcgcgagg ggatcgagcc cggggtgggc gaagaactcc agcatgagat
     1021 ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt
     1081 catagaaggc ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg
     1141 tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat
     1201 gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc
     1261 gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac
     1321 acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg
     1381 caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag
     1441 cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc
     1501 gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc
     1561 gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga
     1621 tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa
     1681 tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc
     1741 cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga
     1801 caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc
     1861 atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc
     1921 ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt
     1981 ctcttgatca gatcttgatc ccctgcgcca tcagatcctt ggcggcaaga aagccatcca
     2041 gtttactttg cagggcttcc caaccttacc agagggcgcc ccagctggca attccggttc
     2101 gcttgctgtc cataaaaccg cccagtctag ctatcgccat gtaagcccac tgcaagctac
     2161 ctgctttctc tttgcgcttg cgttttccct tgtccagata gcccagtagc tgacattcat
     2221 ccggggtcag caccgtttct gcggactggc tttctacgtg ttccgcttcc tttagcagcc
     2281 cttgcgccct gagtgcttgc ggcagcgtga agcttcaggt gagaacatcc ctgcctgaac
     2341 atgagaaaaa acagggtact catactcact tctaagtgac ggctgcatac taaccgcttc
     2401 atacatctcg tagatttctc tggcgattga agggctaaat tcttcaacgc taactttgag
     2461 aatttttgca agcaatgcgg cgttataagc atttaatgca ttgatgccat taaataaagc
     2521 accaacgcct gactgcccca tccccatctt gtctgcgaca gattcctggg ataagccaag
     2581 ttcatttttc tttttttcat aaattgcttt aaggcgacgt gcgtcctcaa gctgctcttg
     2641 tgttaatggt ttcttttttg tgctcatacg ttaaatctat caccgcaagg gataaatatc
     2701 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgca tgtactaagg
     2761 aggttgtatg gaacaacgca taaccctgaa agattatgca atgcgctttg ggcaaaccaa
     2821 gacagctaaa gatctcggcg tatatcaaag cgcgatcaac aaggccattc atgcaggccg
     2881 aaagattttt ttaactataa acgctgatgg aagcgtttat gcggaagagg taaagccctt
     2941 cccgagtaac aaaaaaacaa cagcataaat aaccccgctc ttacacattc cagccctgaa
     3001 aaagggcatc aaattaaacc acacctatgg tgtatgcatt tatttgcata cattcaatca
     3061 attgttatct aaggaaatac ttacatatgg ttcgtgcaaa caaacgcaac gaggctctac
     3121 gaatcgagag tgcgttgctt aacaaaatcg caatgcttgg aactgagaag acagcggaag
     3181 ctgtgggcgt tgataagtcg cagatcagca ggtggaagag ggactggatt ccaaagttct
     3241 caatgctgct tgctgttctt gaatgggggg tcgttgacga cgacatggct cgattggcgc
     3301 gacaagttgc tgcgattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
     3361 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtca ttatgacaaa
     3421 tacagcaaaa atactcaact tcggcagagg taactttgcc ggacaggagc gtaatgtggc
     3481 agatctcgat gatggttacg ccagactatc aaatatgctg cttgaggctt attcgggcgc
     3541 agatctgacc aagcgacagt ttaaagtgct gcttgccatt ctgcgtaaaa cctatgggtg
     3601 gaataaacca atggacagaa tcaccgattc tcaacttagc gagattacaa agttacctgt
     3661 caaacggtgc aatgaagcca agttagaact cgtcagaatg aatattatca agcagcaagg
     3721 cggcatgttt ggaccaaata aaaacatctc agaatggtgc atccctcaaa acgagggaaa
     3781 atcccctaaa acgagggata aaacatccct caaattgggg gattgctatc cctcaaaaca
     3841 gggggacaca aaagacacta ttacaaaaga aaaaagaaaa gattattcgt cagagaattc
     3901 tggcgaatcc tctgaccagc cagaaaacga cctttctgtg gtgaaaccgg atgctgcaat
     3961 tcagagcggc agcaagtggg ggacagcaga agacctgacc gccgcagagt ggatgtttga
     4021 catggtgaag actatcgcac catcagccag aaaaccgaat tttgctgggt gggctaacga
     4081 tatccgcctg atgcgtgaac gtgacggacg taaccaccgc gacatgtgtg tgctgttccg
     4141 ctgggcatgc caggacaact tctggtccgg taacgtgctg agcccggcca aactccgcga
     4201 taagtggacc caactcgaaa tcaaccgtaa caagcaacag gcaggcgtga cagccagcaa
     4261 accaaaactc gacctgacaa acacagactg gatttacggg gtggatctat gaaaaacatc
     4321 gccgcacaga tggttaactt tgaccgtgag cagatgcgtc ggatcgccaa caacatgccg
     4381 gaacagtacg acgaaaagcc gcaggtacag caggtagcgc agatcatcaa cggtgtgttc
     4441 agccagttac tggcaacttt cccggcgagc ctggctaacc gtgaccagaa cgaagtgaac
     4501 gaaatccgtc gccagtgggt tctggctttt cgggaaaacg ggatcaccac gatggaacag
     4561 gttaacgcag gaatgcgcgt agcccgtcgg cagaatcgac catttctgcc atcacccggg
     4621 cagtttgttg catggtgccg ggaagaagca tccgttaccg ccggactgcc aaacgtcagc
     4681 gagctggttg atatggttta cgagtattgc cggaagcgag gcctgtatcc ggatgcggag
     4741 tcttatccgt ggaaatcaaa cgcgcactac tggctggtta ccaacctgta tcagaacatg
     4801 cgggccaatg cgcttactga tgcggaatta cgccgtaagg ccgcagatga gcttgtccat
     4861 atgactgcga gaattaaccg tggtgaggcg atccctgaac cagtaaaaca acttcctgtc
     4921 atgggcggta gacctctaaa tcgtgcacag gctctggcga agatcgcaga aatcaaagct
     4981 aagttcggac tgaaaggagc aagtgtatga cgggcaaaga ggcaattatt cattacctgg
     5041 ggacgcataa tagcttctgt gcgccggacg ttgccgcgct aacaggcgca acagtaacca
     5101 gcataaatca ggccgcggct aaaatggcac gggcaggtct tctggttatc gaaggtaagg
     5161 tctggcgaac ggtgtattac cggtttgcta ccagggaaga acgggaagga aagatgagca
     5221 cgaacctggt ttttaaggag tgtcgccaga gtgccgcgat gaaacgggta ttggcggtat
     5281 atggagttaa aagatgacca tctacattac tgagctaata acaggcctgc tggtaatcgc
     5341 aggccttttt atttggggga gagggaagtc atgaaaaaac taacctttga aattcgatct
     5401 ccagcacatc agcaaaacgc tattcacgca gtacagcaaa tccttccaga cccaaccaaa
     5461 ccaatcgtag taaccattca ggaacgcaac cgcagcttag accaaaacag gaagctatgg
     5521 gcctgcttag gtgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     5581 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     5641 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     5701 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     5761 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     5821 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     5881 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     5941 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
     6001 ttcgagctcg gtacccgggg gcgcgccgga tccttaatta agtctagagt cgactgttta
     6061 aacctgcagg catgcaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     6121 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     6181 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     6241 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     6301 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6361 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6421 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6481 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6541 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6601 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6661 tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt
     6721 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6781 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     6841 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     6901 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     6961 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7021 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7081 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7141 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7201 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     7261 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     7321 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     7381 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7441 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     7501 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     7561 tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     7621 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     7681 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     7741 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     7801 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     7861 cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     7921 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     7981 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     8041 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     8101 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     8161 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     8221 cacatttccc cgaaaagtgc ggtaatgact ccaacttatt gatagtgttt tatgttcaga
     8281 taatgcccga tgactttgtc atgcagctcc accgattttg agaacgacag cgacttccgt
     8341 cccagccgtg ccaggtgctg cctcagattc aggttatgcc gctcaattcg ctgcgtatat
     8401 cgcttgctga ttacgtgcag ctttcccttc aggcgggatt catacagcgg ccagccatcc
     8461 gtcatccata tcaccacgtc aaagggtgac agcaggctca taagacgccc cagcgtcgcc
     8521 atagtgcgtt caccgaatac gtgcgcaaca accgtcttcc ggagactgtc atacgcgtgc
     8581 gtcatatact gactgaaaac gcccgcaccg ttgaagctgc cagcgcgctg gagcaaggcg
     8641 acctgaaacg tatgggcgag ttgatggcgg agtctcatgc ctctatgcgc gatgatttcg
     8701 aaatcaccgt gccgcaaatt gacactctgg tagaaatcgt caaagctgtg attggcgaca
     8761 aaggtggcgt acgcatgacc ggcggcggat ttggcggctg tatcgtcgcg ctgatcccgg
     8821 aagagctggt gcctgccgta cagcaacgtg tcgctgaaca atatgaagca aaaacaggta
     8881 ttaaagagac tttttacgtt tgtaaaccat cacaaggagc aggacagtgc tgaacgaaac
     8941 tcccgcactg gcacccgatg gtcagccgta ccgactgttg atctgcctcg ctggcctgcc
     9001 gcagttcttc aacctcccgg cgcagctttt cgttctcaat ttcagcatcc ctttcggcat
     9061 accattttat gacggcggca gagtcataaa gcacctcatt acccttgcca ccgcctcgca
     9121 gaacgggcat tccctgttcc tgccagttct gaatggtacg gatactcgca ccgaaaatgt
     9181 cagccagctg ctttttgttg acttccattg ttcattccac ggacaaaaac agagaaagga
     9241 aacgacagag gccaaaaagc ctcgctttca gcacctgtcg tttcctttct tttcagaggg
     9301 tattttaaat aaaaacatta agttatgacg aagaagaacg gaaacgcctt aaaccggaaa
     9361 attttcataa atagcgaaaa cccgcg
//
