pDC-KHGT vector (V008062)

LOCUS       V008062                 7173 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008062
VERSION     V008062
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Crivat G, Tokumasu F, Sa JM, Hwang J, Wellems TE.
  TITLE     Tetracysteine-Based Fluorescent Tags to Study Protein Localization
            and Trafficking in Plasmodium falciparum-Infected Erythrocytes
  JOURNAL   PLoS ONE 6 (8), E22975 (2011)
   PUBMED   21860664
REFERENCE   2  (bases 1 to 7173)
  AUTHORS   Crivat G, Tokumasu F, Sa JM, Hwang J, Wellems TE.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-FEB-2011) LMVR, NIAID, 12735 Twinbrook Pkwy, 3E10C,
            Rockville, MD 20852, USA
REFERENCE   3  (bases 1 to 7173)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7173)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2011"; volume: "6"; issue: "8"; pages: "E22975"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (23-FEB-2011) LMVR, NIAID, 12735 Twinbrook Pkwy, 3E10C, Rockville,
            MD 20852, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(878..895)
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             complement(896..925)
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             complement(942..959)
                     /label="tetracysteine tag"
                     /note="tetracysteine peptide that binds biarsenical
                     labeling reagents"
     CDS             complement(984..1694)
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     CDS             complement(1710..1736)
                     /label="9xHis"
                     /note="9xHis affinity tag"
     CDS             complement(1797..1814)
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             complement(1836..1853)
                     /label="HQ tag"
                     /note="HQHQHQ metal affinity tag"
     CDS             complement(1851..1871)
                     /label="7xHis"
                     /note="6xHis affinity tag"
     CDS             3307..3867
                     /gene="DHFR"
                     /label="Dihydrofolate reductase"
                     /note="Dihydrofolate reductase from Homo sapiens.
                     Accession#: P00374"
     promoter        complement(4491..4508)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4515..4531)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5005..5109
                     /label="AmpR promoter"
     CDS             5110..5967
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6141..6729
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    7017..7038
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7053..7083
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7091..7107
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7115..7131
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        7149..7167
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"