LOCUS       V008062                 7173 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008062
VERSION     V008062
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Crivat G, Tokumasu F, Sa JM, Hwang J, Wellems TE.
  TITLE     Tetracysteine-Based Fluorescent Tags to Study Protein Localization
            and Trafficking in Plasmodium falciparum-Infected Erythrocytes
  JOURNAL   PLoS ONE 6 (8), E22975 (2011)
   PUBMED   21860664
REFERENCE   2  (bases 1 to 7173)
  AUTHORS   Crivat G, Tokumasu F, Sa JM, Hwang J, Wellems TE.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-FEB-2011) LMVR, NIAID, 12735 Twinbrook Pkwy, 3E10C,
            Rockville, MD 20852, USA
REFERENCE   3  (bases 1 to 7173)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7173)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2011"; volume: "6"; issue: "8"; pages: "E22975"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (23-FEB-2011) LMVR, NIAID, 12735 Twinbrook Pkwy, 3E10C, Rockville,
            MD 20852, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(878..895)
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             complement(896..925)
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             complement(942..959)
                     /label="tetracysteine tag"
                     /note="tetracysteine peptide that binds biarsenical
                     labeling reagents"
     CDS             complement(984..1694)
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     CDS             complement(1710..1736)
                     /label="9xHis"
                     /note="9xHis affinity tag"
     CDS             complement(1797..1814)
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             complement(1836..1853)
                     /label="HQ tag"
                     /note="HQHQHQ metal affinity tag"
     CDS             complement(1851..1871)
                     /label="7xHis"
                     /note="6xHis affinity tag"
     CDS             3307..3867
                     /gene="DHFR"
                     /label="Dihydrofolate reductase"
                     /note="Dihydrofolate reductase from Homo sapiens.
                     Accession#: P00374"
     promoter        complement(4491..4508)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4515..4531)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5005..5109
                     /label="AmpR promoter"
     CDS             5110..5967
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6141..6729
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    7017..7038
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7053..7083
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7091..7107
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7115..7131
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        7149..7167
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatcctatt tgatgaatta actacactta
       61 aaataataca attattatta aatttttttt tgatttattt attaattttt aaacttaatc
      121 atttgtattt gggaggaatt atatatatct ttataattat tttatttttt tttatttttt
      181 tattttttta ttattattat ttttttttat tttttttttt tactgtatca aagaaaaacc
      241 tttaaaaaaa aaattataat ttccccatct tactatattt ttaatacata cgttttaagg
      301 aattaaatta gacaaaagct atattatgct ttacatataa ttagaattta taaacgtttg
      361 gttattagat atttcatgtc tcagtaaagt ctttcaatac atatgtaaaa aaatatatat
      421 gaatacacat aagttgttaa tatattttat atgcataaat gtataaatat atatatatat
      481 atatatatat gtatgtatgt atatgtgtgt atatgaaatt atttcaatgt ttaatttttt
      541 aaattttaat tttttttttt tttttttttt ttattatgta tattgatctt tattatttaa
      601 atattacttt tttcgttttt tcttcttttt attatttttt ttttttttta tattttatac
      661 aaatggtaat tcaaataaaa ggtataaatt tatatttaat tttcttttat ggataaataa
      721 aagaaaaata taaatatata aaaatataaa aatatatata tgtatattgg ggtgatgata
      781 aaatgaaaga taatatatat atatatatat ctttattttt ttttttttgt agaccccatt
      841 gtgagtacat aaatatatta tataactcga gtcattaatg gtgatggtga tggtgcaagt
      901 cttcttcaga aataagtttt tgttccccgg gggccgccac cgcaacagcc aggacaacag
      961 ccaccagcac cggtacggat atctttgtat agttcatcca tgccatgtgt aatcccagca
     1021 gctgttacaa actcaagaag gaccatgtgg tctctctttt cgttgggatc tttcgaaagg
     1081 gcagattgtg tggacaggta atggttgtct ggtaaaagga cagggccatc gccaattgga
     1141 gtattttgtt gataatggtc tgctagttga acgcttccat cttcaatgtt gtgtctaatt
     1201 ttgaagttaa ctttgattcc attcttttgt ttgtctgcca tgatgtatac attgtgtgag
     1261 ttatagttgt attccaattt gtgtccaaga atgtttccat cttctttaaa atcaatacct
     1321 tttaactcga ttctattaac aagggtatca ccttcaaact tgacttcagc acgtgtcttg
     1381 tagttcccgt catctttgaa aaatatagtt ctttcctgta cataaccttc gggcatggca
     1441 ctcttgaaaa agtcatgctg tttcatatga tctgggtatc tcgcaaagca ttgaagacca
     1501 tacgcgaaag tagtgacaag tgttggccat ggaacaggta gttttccagt agtgcaaata
     1561 aatttaaggg taagttttcc gtatgttgca tcaccttcac cctctccact gacagaaaat
     1621 ttgtgcccat taacatcacc atctaattca acaagaattg ggacaactcc agtgaaaagt
     1681 tcttctcctt tactcctagg ttgtaattga tgatggtggt gatgatggtg atggtgtgct
     1741 tgtttttgac catgtacttg ttggtgaacc tgtggtgctt ggtgatttac ttttccatga
     1801 tgatgatggt ggtgtgcttg gtgtggagct tggtgttggt gttgatgttg atggtgatgg
     1861 tggtgatggt gttcatgttg cttttgtgct aaagttctct tatttctgaa atcgaaggag
     1921 tcaccggatc cgtttccatt attgcagtta ttagagcact tcaaaaccca tactaaaaaa
     1981 gagactaaaa gaattttagt aaaaacaggg aaagccttct ttctcctcaa agtatttttg
     2041 ttcttaaaac ttttcatact agtctcgagg gatcctgata tatttctatt aggtatttat
     2101 tattataaaa tataaatctt gaatgataat aaataaaata ttagttattc cttttctagt
     2161 ttaaaatata catattataa atatatatat atatatatat atttttattg tgacaagaat
     2221 atataattat aaattatatt atttattttt gtattttttt tttttttttt ttttttttct
     2281 ttttttgttt tatttttctt tttttttata aatattattt ttttctttta tcatgcacat
     2341 tggaataata cattaatata tatatatata ttatattata catatattga ataatgttta
     2401 taaaaaatgc ataacttata tgaatataat tttttttaaa tatgacaaaa agaaaaaaaa
     2461 aaaaaaccaa aaaaaattaa aattgaaatg aaatatataa atatattatt tatatatatt
     2521 atacattgtt taatactact acatgtatat atatatatta tatatatata tatatatcaa
     2581 ttttttcaaa aataaattaa tataaaaaga ggggaaaaaa aaaaaaaaaa aaaaaaaaag
     2641 ataattaagt aagcatttaa aaatatataa attgataata tataaaatta atcacatata
     2701 agcttgcatg cttagctaat tcgcttgtaa gaggtactct cgtttatgca aaactatttg
     2761 atatagcatt ttaacaagta cacatatata tatgtaatat atatactata tatatctatt
     2821 gcatgtgtac taagcatgtg catggcatcc cctttttctc gtgtttaaaa cagtttgtat
     2881 gataaaatat aaaggatttg aaaaagagaa aaaaatatat gatctcatcc tatatagcgc
     2941 cataattttt atttgggttg aataaaattt tctactaaat ttaggtgtaa gtaaaataat
     3001 ggaatatata taagtacaat aaaaaagtgc ataaattaaa aaatttttat aataaatatt
     3061 ttttttaaaa aagtcaataa taatattaaa tatatataac acaggattat atatgttcac
     3121 tacaattttt tatattataa tataaattct tttcaatttt cattttattt tacatacact
     3181 ttcctttttt gtcactatat tttaatattc acatatttag tttaaatact ggctatttct
     3241 ttctacattt gctagtaaca attgtgtagt gcttatatat atacacacac ctaaaactta
     3301 caaaccatgg ttggttcgct aaactgcatc gtcgctgtgt cccagaacat gggcatcggc
     3361 aagaacgggg acctgccctg gccaccgctc aggaacgaat ttagatattt ccagagaatg
     3421 accacaacct cttcagtaga aggtaagcag aatctggtga ttatgggtaa gaagacctgg
     3481 ttctccattc ctgagaagaa tcgaccttta aagggtagaa ttaatttagt tctcagcaga
     3541 gaactcaagg aacctccaca aggagctcat tttctttcca gaagtctaga tgatgcctta
     3601 aaacttactg aacaaccaga attagcaaat aaagtagaca tggtctggat agttggtggc
     3661 agttctgttt ataaggaagc catgaatcac ccaggccatc ttaaactatt tgtgacaagg
     3721 atcatgcaag actttgaaag tgacacgttt tttccagaaa ttgatttgga gaaatataaa
     3781 cttctgccag aatacccagg tgttctctct gatgtccagg aggagaaagg cattaagtac
     3841 aaatttgaag tatatgagaa gaatgattaa ccgcggggta ccccattaaa tttatttaat
     3901 aatagattaa aaatattata aaaataaaaa cataaacaca gaaattacaa aaaaaataca
     3961 tatgaatttt ttttttgtaa tcttccttat aaatatagaa taatgaatca tataaaacat
     4021 atcatttttc atttatttac atttaaaatt attgtttcag tatctttaat ttattatgta
     4081 tatataaaaa taacttacaa ttttattaat aaacaatata tgtttattaa ttcatgtttt
     4141 gtaatttatg ggatagcgat tttttttact gtctgtattt ttctttttta attatgtttt
     4201 aattgtattt tatttttatt attgttcttt ttatagtatt attttaaaac aaaatgtttt
     4261 ttctaagaac ttataataat aataaatata aattttaata aaaattatat ttatctttta
     4321 caatatgaac ataaagtaca acattaatat atagctttta atatttttat tcctaatcat
     4381 gtaaatctta aatttttctt tttaaacata tgttaaatat ttatttctca ttatatataa
     4441 ggacatattt attaaaccgc agagaaatct agaggtaccg agctcgaatt ctatagtgag
     4501 tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     4561 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     4621 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
     4681 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     4741 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     4801 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     4861 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     4921 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     4981 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     5041 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     5101 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     5161 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     5221 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     5281 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     5341 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     5401 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     5461 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     5521 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     5581 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     5641 accacgatgc ctgtagcaat gccaacaacg ttgcgcaaac tattaactgg cgaactactt
     5701 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     5761 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     5821 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     5881 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     5941 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     6001 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     6061 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6121 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6181 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6241 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     6301 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6361 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6421 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6481 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6541 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6601 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6661 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6721 ctatcgaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     6781 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     6841 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     6901 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     6961 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     7021 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     7081 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     7141 gccaagctat ttaggtgaca ctatagaata ctc
//
