pDB790 vector (V008065)

LOCUS       40924_14185        5996 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Gene trapping vector pDB790, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5996)
  AUTHORS   Balciuniene J, Nagelberg D, Walsh KT, Camerota D, Georlette D, 
            Biemar F, Bellipanni G, Balciunas D.
  TITLE     Efficient disruption of Zebrafish genes using a Gal4-containing gene
            trap
  JOURNAL   BMC Genomics 14, 619 (2013)
  PUBMED    24034702
REFERENCE   2  (bases 1 to 5996)
  AUTHORS   Balciuniene J, Balciunas D.
  TITLE     Gene trapping using gal4 in zebrafish
  JOURNAL   J Vis Exp 79, E50113 (2013)
  PUBMED    24121167
REFERENCE   3  (bases 1 to 5996)
  AUTHORS   Balciunas D.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2013) Department of Biology, Temple University, 
            1900 N 12th St, Philadelphia, PA 19122, USA
REFERENCE   4  (bases 1 to 5996)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5996)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics 14, 619 (2013)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "J Vis Exp 
            79, E50113 (2013)"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (15-NOV-2013) Department of Biology, Temple University, 1900 N 12th 
            St, Philadelphia, PA 19122, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..202)
                     /label=miniTol2 3'
                     /note="miniTol2 3'"
     misc_feature    210..414
                     /label=X. laevis crygc promoter
                     /note="X. laevis crygc promoter"
     misc_binding    210..414
                     /label=14X Gal4 UAS binding site
                     /bound_moiety="14X Gal4 UAS"
     CDS             438..1151
                     /label=GFP
                     /note="green fluorescent protein"
     polyA_signal    1198..1332
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      1202..1393
                     /label=SV40 late polyA
                     /note="SV40 late polyA"
                     /regulatory_class="polyA_signal_sequence"
     CDS             1901..2575
                     /label=mRFP1
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
     polyA_signal    complement(2589..2723)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(2796..3056)
                     /label=miniTol2 5'
                     /note="miniTol2 5'"
     promoter        complement(3069..3087)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3094..3110)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3182..3250
                     /label=partial LacZ alpha
                     /note="partial LacZ alpha"
     rep_origin      3251..3706
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3784..3888
                     /label=AmpR promoter
     CDS             3889..4746
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4920..5508
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5796..5817
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5832..5862
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5870..5886
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5894..5910
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5928..5946
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"