LOCUS       40924_14185        5996 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Gene trapping vector pDB790, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5996)
  AUTHORS   Balciuniene J, Nagelberg D, Walsh KT, Camerota D, Georlette D, 
            Biemar F, Bellipanni G, Balciunas D.
  TITLE     Efficient disruption of Zebrafish genes using a Gal4-containing gene
            trap
  JOURNAL   BMC Genomics 14, 619 (2013)
  PUBMED    24034702
REFERENCE   2  (bases 1 to 5996)
  AUTHORS   Balciuniene J, Balciunas D.
  TITLE     Gene trapping using gal4 in zebrafish
  JOURNAL   J Vis Exp 79, E50113 (2013)
  PUBMED    24121167
REFERENCE   3  (bases 1 to 5996)
  AUTHORS   Balciunas D.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2013) Department of Biology, Temple University, 
            1900 N 12th St, Philadelphia, PA 19122, USA
REFERENCE   4  (bases 1 to 5996)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5996)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics 14, 619 (2013)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "J Vis Exp 
            79, E50113 (2013)"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (15-NOV-2013) Department of Biology, Temple University, 1900 N 12th 
            St, Philadelphia, PA 19122, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..202)
                     /label=miniTol2 3'
                     /note="miniTol2 3'"
     misc_feature    210..414
                     /label=X. laevis crygc promoter
                     /note="X. laevis crygc promoter"
     misc_binding    210..414
                     /label=14X Gal4 UAS binding site
                     /bound_moiety="14X Gal4 UAS"
     CDS             438..1151
                     /label=GFP
                     /note="green fluorescent protein"
     polyA_signal    1198..1332
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      1202..1393
                     /label=SV40 late polyA
                     /note="SV40 late polyA"
                     /regulatory_class="polyA_signal_sequence"
     CDS             1901..2575
                     /label=mRFP1
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
     polyA_signal    complement(2589..2723)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(2796..3056)
                     /label=miniTol2 5'
                     /note="miniTol2 5'"
     promoter        complement(3069..3087)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3094..3110)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3182..3250
                     /label=partial LacZ alpha
                     /note="partial LacZ alpha"
     rep_origin      3251..3706
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3784..3888
                     /label=AmpR promoter
     CDS             3889..4746
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4920..5508
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5796..5817
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5832..5862
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5870..5886
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5894..5910
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5928..5946
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 cagaggtgta aaaagtactc aaaaatttta ctcaagtgaa agtacaagta cttagggaaa
       61 attttactca attaaaagta aaagtatctg gctagaatct tacttgagta aaagtaaaaa
      121 agtactccat taaaattgta cttgagtatt aaggaagtaa aagtaaaagc aagaaagaaa
      181 actagagatt cttgtttaag ctagctagcg aacaaaagta ccagcatgaa ctaagaaatg
      241 actgtcacag cacaacatca aacacaatgc aagacaaagg ttctctgctt ttgccttact
      301 tgacagaaaa agtaagctct aggttcatcg catgcagaca gcagcaatac atgacatata
      361 taaaggctgg ctcttttctc ccttccattg aactgaaact tccactcagt cagacttgcg
      421 ggggatccac agccaccatg agtaaaggag aagaactttt cactggagtt gtcccaattc
      481 ttgttgaatt agatggtgat gttaatgggt acaaattttc tgtcagtgga gagggtgaag
      541 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
      601 ttccatggcc aacacttgtc actactctca cttatggtgt tcaatgcttt tcaagatatc
      661 cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg
      721 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg
      781 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
      841 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
      901 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa
      961 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt
     1021 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga
     1081 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg
     1141 aactatacaa ataaatgtcc agacctgcag gaattcgata tcaagcttat cgatgatgat
     1201 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     1261 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     1321 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     1381 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatcatcgc
     1441 tagcttaggc ctccaaggcg gagtactgtc ctccgggctg gcggagtact gtcctccggc
     1501 aaggtcggag tactgtcctc cgacactaga ggtcggagta ctgtcctccg acgcaaggcg
     1561 gagtactgtc ctccgggctg cggagtactg tcctccggca aggtcggagt actgtcctcc
     1621 gacactagag gtcggagtac tgtcctccga cgcaaggtcg gagtactgtc ctccgacact
     1681 agaggtcgga gtactgtcct ccgacgcaag gtcggagtac tgtcctccga cactagaggt
     1741 cggagtactg tcctccgacg caaggcggag tactgtcctc cgggctggcg gagtactgtc
     1801 ctccggcaag ggtcgactct agagggtata taatggatcc catcgcgtct cagcctcact
     1861 ttgagctcct ccacacgaat tcacccgggt accggtagaa atggcctcct ccgaggacgt
     1921 catcaaggag ttcatgcgct tcaaggtgcg catggagggc tccgtgaacg gccacgagtt
     1981 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     2041 ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccctc agttccagta
     2101 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
     2161 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
     2221 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
     2281 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac
     2341 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagatga ggctgaagct
     2401 gaaggacggc ggccactacg acgccgaggt caagaccacc tacatggcca agaagcccgt
     2461 gcagctgccc ggcgcctaca agaccgacat caagctggac atcacctccc acaacgagga
     2521 ctacaccatc gtggaacagt acgagcgcgc cgagggccgc cactccaccg gcgcctaaga
     2581 aatcggtaga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     2641 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     2701 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     2761 agggggaggt gtgggaggtt ttttaattcg cggccaaatt aaactgggca tcagcgcaat
     2821 tcaattggtt tggtaatagc aagggaaaat agaatgaagt gatctccaaa aaataagtac
     2881 tttttgactg taaataaaat tgtaaggagt aaaaagtact tttttttcta aaaaaatgta
     2941 attaagtaaa agtaaaagta ttgattttta attgtactca agtaaagtaa aaatccccaa
     3001 aaataatact taagtacagt aatcaagtaa aattactcaa gtactttaca cctctgggcc
     3061 caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga
     3121 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     3181 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     3241 tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     3301 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     3361 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     3421 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     3481 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     3541 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     3601 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     3661 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt
     3721 ttctccttac gcatctgtgc ggtatttcac accgcatcag gtggcacttt tcggggaaat
     3781 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     3841 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     3901 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     3961 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4021 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     4081 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     4141 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     4201 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     4261 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     4321 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     4381 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     4441 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     4501 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     4561 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     4621 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     4681 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     4741 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     4801 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     4861 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     4921 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     4981 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5041 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     5101 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     5161 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     5221 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     5281 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     5341 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     5401 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     5461 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     5521 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     5581 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     5641 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     5701 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     5761 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     5821 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     5881 taacaatttc acacaggaaa cagctatgac catgattacg ccaagctatt taggtgacac
     5941 tatagaatac tcaagctatg catccaacgc gttgggagct ctcccatatg gtcgag
//
