pDB783 vector (V008066)

LOCUS       40924_14180        6888 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Gene trap vector pDB783, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6888)
  AUTHORS   Balciuniene J, Nagelberg D, Walsh K, Camerota D, Georlette D, Biemar
            F, Bellipanni G, Balciunas D.
  TITLE     Efficient disruption of Zebrafish genes using a Gal4-containing gene
            trap
  JOURNAL   BMC Genomics 14 (1), 619 (2013)
  PUBMED    24034702
REFERENCE   2  (bases 1 to 6888)
  AUTHORS   Darius B.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2013) Department of Biology, Temple University, 
            1900 N 12th St, Philadelphia, PA 19122, USA
REFERENCE   3  (bases 1 to 6888)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6888)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics"; date: "2013"; volume: "14"; issue: "1"; pages: "619"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-NOV-2013) Department of Biology, Temple University, 1900 N 12th 
            St, Philadelphia, PA 19122, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  complement(2..203)
                     /mobile_element_type="transposon:Tol2"
                     /label=ol2
                     /note="miniTol2 3'"
     misc_feature    205..222
                     /label=I-Sce
                     /note="I-Sce"
     protein_bind    221..254
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    263..402
                     /label=carp b-actin intron 1 splice acceptor
                     /note="carp b-actin intron 1 splice acceptor"
     CDS             423..860
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     misc_feature    1080..1678
                     /label=Danio rerio b-actin 3' UTR
                     /note="Danio rerio b-actin 3' UTR"
     misc_feature    1659..2084
                     /label=Danio rerio b-actin p(A)
                     /note="Danio rerio b-actin p(A)"
     protein_bind    2089..2122
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     polyA_signal    2203..2337
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2342..2359
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(2382..3098)
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    complement(3205..3574)
                     /label=14x Gal4 UAS
                     /note="14x Gal4 UAS"
     misc_feature    3588..3621
                     /label=FRT
                     /note="FRT"
     protein_bind    3588..3621
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     misc_feature    3632..3665
                     /label=loxP
                     /note="loxP"
     protein_bind    complement(3632..3665)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    complement(3666..3683)
                     /label=I-Sce
                     /note="I-Sce"
     mobile_element  complement(3689..3949)
                     /mobile_element_type="transposon:Tol2"
                     /label=ol2
                     /note="miniTol2 5'"
     promoter        complement(3962..3980)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3987..4003)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4075..4143
                     /codon_start=1
                     /product="LacZ alpha"
                     /label=LacZ alpha
                     /protein_id="AHE73660.1"
                     /translation="WRNSEEARTDRPSQQLRSLNGEW"
     rep_origin      4144..4599
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4677..4781
                     /label=AmpR promoter
     CDS             4782..5639
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5813..6401
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6689..6710
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6725..6755
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6763..6779
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6787..6803
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6821..6839
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"