LOCUS       40924_14180        6888 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Gene trap vector pDB783, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6888)
  AUTHORS   Balciuniene J, Nagelberg D, Walsh K, Camerota D, Georlette D, Biemar
            F, Bellipanni G, Balciunas D.
  TITLE     Efficient disruption of Zebrafish genes using a Gal4-containing gene
            trap
  JOURNAL   BMC Genomics 14 (1), 619 (2013)
  PUBMED    24034702
REFERENCE   2  (bases 1 to 6888)
  AUTHORS   Darius B.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2013) Department of Biology, Temple University, 
            1900 N 12th St, Philadelphia, PA 19122, USA
REFERENCE   3  (bases 1 to 6888)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6888)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics"; date: "2013"; volume: "14"; issue: "1"; pages: "619"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-NOV-2013) Department of Biology, Temple University, 1900 N 12th 
            St, Philadelphia, PA 19122, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  complement(2..203)
                     /mobile_element_type="transposon:Tol2"
                     /label=ol2
                     /note="miniTol2 3'"
     misc_feature    205..222
                     /label=I-Sce
                     /note="I-Sce"
     protein_bind    221..254
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    263..402
                     /label=carp b-actin intron 1 splice acceptor
                     /note="carp b-actin intron 1 splice acceptor"
     CDS             423..860
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     misc_feature    1080..1678
                     /label=Danio rerio b-actin 3' UTR
                     /note="Danio rerio b-actin 3' UTR"
     misc_feature    1659..2084
                     /label=Danio rerio b-actin p(A)
                     /note="Danio rerio b-actin p(A)"
     protein_bind    2089..2122
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     polyA_signal    2203..2337
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2342..2359
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(2382..3098)
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    complement(3205..3574)
                     /label=14x Gal4 UAS
                     /note="14x Gal4 UAS"
     misc_feature    3588..3621
                     /label=FRT
                     /note="FRT"
     protein_bind    3588..3621
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     misc_feature    3632..3665
                     /label=loxP
                     /note="loxP"
     protein_bind    complement(3632..3665)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    complement(3666..3683)
                     /label=I-Sce
                     /note="I-Sce"
     mobile_element  complement(3689..3949)
                     /mobile_element_type="transposon:Tol2"
                     /label=ol2
                     /note="miniTol2 5'"
     promoter        complement(3962..3980)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3987..4003)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4075..4143
                     /codon_start=1
                     /product="LacZ alpha"
                     /label=LacZ alpha
                     /protein_id="AHE73660.1"
                     /translation="WRNSEEARTDRPSQQLRSLNGEW"
     rep_origin      4144..4599
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4677..4781
                     /label=AmpR promoter
     CDS             4782..5639
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5813..6401
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6689..6710
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6725..6755
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6763..6779
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6787..6803
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6821..6839
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gcagaggtgt aaaaagtact caaaaatttt actcaagtga aagtacaagt acttagggaa
       61 aattttactc aattaaaagt aaaagtatct ggctagaatc ttacttgagt aaaagtaaaa
      121 aagtactcca ttaaaattgt acttgagtat taaggaagta aaagtaaaag caagaaagaa
      181 aactagagat tcttgtttaa gctgtaggga taacagggta ataacttcgt ataatgtatg
      241 ctatacgaag ttatcagcat gccacccact agcggtcaga ctgcagattg cagcacgaaa
      301 caggaagctg actccacatg gtcacatgct cactgaagtg ttgacttccc tgacagctgt
      361 gcactttcta aaccggtttt ctcattcatt tacagttcag ccggaattca cccgggtacc
      421 agaagctact gtcttctatc gaacaagcat gcgatatttg ccgacttaaa aagctcaagt
      481 gctccaaaga aaaaccgaag tgcgccaagt gtctgaagaa caactgggag tgtcgctact
      541 ctcccaaaac caaaaggtct ccgctgacta gggcacatct gacagaagtg gaatcaaggc
      601 tagaaagact ggaacagcta tttctactga tttttcctcg agaagacctt gacatgattt
      661 tgaaaatgga ttctttacag gatataaaag cattgttaac aggattattt gtacaagata
      721 atgtgaataa agatgccgtc acagatagat tggcttcagt ggagactgat atgcctctaa
      781 cattgagaca gcatagaata agtgcgacat catcatcgga agagagtagt aacaaaggtc
      841 aaagacagtt gactgtatcg tcgaggtcga ccccgggaat tcagatctct cgagccgccc
      901 ccccgaccga tgtcagcctg ggggacgagc tccacttaga cggcgaggac gtggcgatgg
      961 cgcatgccga cgcgctagac gatttcgatc tggacatgtt gggggacggg gattccccgg
     1021 gtccgggatt taccccccac gactccgccc cctacggcgc tctggatatg taaatcgata
     1081 cggactgtta ccacttcacg ccgactcaaa ctgcgcagag aaacttcaaa cgacaacatt
     1141 ggcatggctt ttgttatttt tggcgcttga ctcaggatct aaaaactgga acggtgaagg
     1201 tgacggcaat gttttttggc aaataagcat ccccgaagtt ctacaatgca tctgaggact
     1261 ccatgttttg ttttctttag tcattccaaa tgtttgttaa atgcattgtt ccgaaactta
     1321 tttgcctcta tgaaggctgc ccagtaattg ggagcatact taacattgta gtattgtatg
     1381 taaattatgt aacaaaacaa tgtctgggtt tttgtacttt tcagccttaa aatcttgggt
     1441 ttattttttt ttctttgttc caaaaaaacg aagctttacc attcaagatg taaaggtttc
     1501 cattccccct gggcatattg taaaagctgt gtggaacgtg gcggtgccag acatttggtg
     1561 gggccaacct gtacactgac taattcaatt ccaataaaag tgcacatgta agacatccta
     1621 cccctgtctc atttcctgtc ttggctctga gtggcttcaa taaaaatggc ttttgtgaag
     1681 ttgcaccatg ttaattacat ttcagcactt tcacaccagg tttttgacta atttgaattt
     1741 atcgaagttt gacctttttt tttttttttc cttcaagcta atttgaggtt atcaaaccat
     1801 ttcattggag gaaacttaat gtttaaaagc ataaattcga aacatgattc atttatttaa
     1861 ctgcttaatg gtgaaacaaa cttcaagtaa tacaccaagt gttccctcat atgaaggtag
     1921 ctaactgcaa acggccttaa ctttccatct taagttgagc tacataaagg ctttaacagg
     1981 aaggaagagc cagtttaaaa gtcaaaccac catgactgat ataaattctt gtccatataa
     2041 ccttgcgttt cacacaatat agctatctac tgctaaataa attatcgcga agttcctatt
     2101 ctctagaaag tataggaact tcctaggagc ggccgcgaat taaaaaacct cccacacctc
     2161 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     2221 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     2281 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctac
     2341 gtaatacgac tcactatagt tctagagtcg cggccgcttt acttgtacag ctcgtccatg
     2401 ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg
     2461 ttggggtctt tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg
     2521 gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc
     2581 tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt tcttctgctt gtcggccatg
     2641 atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc
     2701 tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc
     2761 acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg
     2821 tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg
     2881 ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc
     2941 ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc
     3001 tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc
     3061 accaccccgg tgaacagctc ctcgcccttg ctcaccatgg tggcggcaag ctccttgaat
     3121 ttcgaggtcg agggaattcg tgtggaggag ctcaaagtga ggctgagacg cgatgggatc
     3181 cattatatac cctctagagt cgacccttgc cggaggacag tactccgcca gcccggagga
     3241 cagtactccg ccttgcgtcg gaggacagta ctccgacctc tagtgtcgga ggacagtact
     3301 ccgaccttgc gtcggaggac agtactccga cctctagtgt cggaggacag tactccgacc
     3361 ttgcgtcgga ggacagtact ccgacctcta gtgtcggagg acagtactcc gaccttgccg
     3421 gaggacagta ctccgcagcc cggaggacag tactccgcct tgcgtcggag gacagtactc
     3481 cgacctctag tgtcggagga cagtactccg accttgccgg aggacagtac tccgccagcc
     3541 cggaggacag tactccgcct tggaggccta agctgctccg gagatctgaa gttcctattc
     3601 tctagaaagt ataggaactt cttaattaac gataacttcg tataatgtat gctatacgaa
     3661 gttatattac cctgttatcc ctaccatgaa attaaactgg gcatcagcgc aattcaattg
     3721 gtttggtaat agcaagggaa aatagaatga agtgatctcc aaaaaataag tactttttga
     3781 ctgtaaataa aattgtaagg agtaaaaagt actttttttt ctaaaaaaat gtaattaagt
     3841 aaaagtaaaa gtattgattt ttaattgtac tcaagtaaag taaaaatccc caaaaataat
     3901 acttaagtac agtaatcaag taaaattact caagtacttt acacctctgg gcccaattcg
     3961 ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa
     4021 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     4081 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     4141 tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4201 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     4261 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     4321 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     4381 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     4441 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     4501 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     4561 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc ctgatgcggt attttctcct
     4621 tacgcatctg tgcggtattt cacaccgcat caggtggcac ttttcgggga aatgtgcgcg
     4681 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     4741 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     4801 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     4861 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     4921 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     4981 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     5041 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     5101 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     5161 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     5221 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     5281 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     5341 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     5401 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     5461 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     5521 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     5581 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     5641 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     5701 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     5761 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     5821 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     5881 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     5941 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6001 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6061 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6121 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6181 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     6241 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     6301 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     6361 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     6421 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     6481 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     6541 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     6601 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     6661 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     6721 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     6781 ttcacacagg aaacagctat gaccatgatt acgccaagct atttaggtga cactatagaa
     6841 tactcaagct atgcatccaa cgcgttggga gctctcccat atggtcga
//
