pCYC1lac vector (V008077)

LOCUS       40924_14075        7658 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Integrative expression vector pCYC1lac, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7658)
  AUTHORS   Vickers CE, Bydder SF, Nielsen LK.
  TITLE     Yeast expression vectors with dominant markers and dual gene 
            expression cassettes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7658)
  AUTHORS   Bydder SF, Vickers CE.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2013) AIBN, The University of Queensland, Crn 
            College and Cooper Roads (Bldg 75), St Lucia, QLD 4072, Australia
REFERENCE   3  (bases 1 to 7658)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7658)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-JUL-2013) AIBN, The University of Queensland, Crn College and 
            Cooper Roads (Bldg 75), St Lucia, QLD 4072, Australia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7658
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             18..3089
                     /label=lacZ
                     /note="beta-galactosidase"
     terminator      3093..3340
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(3807..4607)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(4608..4828)
                     /label=URA3 promoter
     promoter        5113..5217
                     /label=AmpR promoter
     CDS             5218..6075
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6249..6837
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    7125..7146
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7161..7191
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7199..7215
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7223..7239
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7260..7278
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     7307..7323
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      7366..7656
                     /label=CYC1 promoter from Saccharomyces cerevisiae
                     /note="CYC1 promoter from Saccharomyces cerevisiae"
                     /regulatory_class="promoter"