LOCUS       40924_14075        7658 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Integrative expression vector pCYC1lac, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7658)
  AUTHORS   Vickers CE, Bydder SF, Nielsen LK.
  TITLE     Yeast expression vectors with dominant markers and dual gene 
            expression cassettes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7658)
  AUTHORS   Bydder SF, Vickers CE.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2013) AIBN, The University of Queensland, Crn 
            College and Cooper Roads (Bldg 75), St Lucia, QLD 4072, Australia
REFERENCE   3  (bases 1 to 7658)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7658)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-JUL-2013) AIBN, The University of Queensland, Crn College and 
            Cooper Roads (Bldg 75), St Lucia, QLD 4072, Australia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7658
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             18..3089
                     /label=lacZ
                     /note="beta-galactosidase"
     terminator      3093..3340
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(3807..4607)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(4608..4828)
                     /label=URA3 promoter
     promoter        5113..5217
                     /label=AmpR promoter
     CDS             5218..6075
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6249..6837
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    7125..7146
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7161..7191
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7199..7215
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7223..7239
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7260..7278
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     7307..7323
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      7366..7656
                     /label=CYC1 promoter from Saccharomyces cerevisiae
                     /note="CYC1 promoter from Saccharomyces cerevisiae"
                     /regulatory_class="promoter"
ORIGIN
        1 tctagagcgg ccgcaccatg accatgatta cggattcact ggccgtcgtt ttacaacgtc
       61 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
      121 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
      181 tgaatggcga atggcgcttt gcctggtttc cggcaccaga agcggtgccg gaaagctggc
      241 tggagtgcga tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg
      301 gttacgatgc gcccatctac accaacgtga cctatcccat tacggtcaat ccgccgtttg
      361 ttcccacgga gaatccgacg ggttgttact cgctcacatt taatgttgat gaaagctggc
      421 tacaggaagg ccagacgcga attatttttg atggcgttaa ctcggcgttt catctgtggt
      481 gcaacgggcg ctgggtcggt tacggccagg acagtcgttt gccgtctgaa tttgacctga
      541 gcgcattttt acgcgccgga gaaaaccgcc tcgcggtgat ggtgctgcgc tggagtgacg
      601 gcagttatct ggaagatcag gatatgtggc ggatgagcgg cattttccgt gacgtctcgt
      661 tgctgcataa accgactaca caaatcagcg atttccatgt tgccactcgc tttaatgatg
      721 atttcagccg cgctgtactg gaggctgaag ttcagatgtg cggcgagttg cgtgactacc
      781 tacgggtaac agtttcttta tggcagggtg aaacgcaggt cgccagcggc accgcgcctt
      841 tcggcggtga aattatcgat gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga
      901 acgtcgaaaa cccgaaactg tggagcgccg aaatcccgaa tctctatcgt gcggtggttg
      961 aactgcacac cgccgacggc acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg
     1021 aggtgcggat tgaaaatggt ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg
     1081 ttaaccgtca cgagcatcat cctctgcatg gtcaggtcat ggatgagcag acgatggtgc
     1141 aggatatcct gctgatgaag cagaacaact ttaacgccgt gcgctgttcg cattatccga
     1201 accatccgct gtggtacacg ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca
     1261 atattgaaac ccacggcatg gtgccaatga atcgtctgac cgatgatccg cgctggctac
     1321 cggcgatgag cgaacgcgta acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga
     1381 tcatctggtc gctggggaat gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct
     1441 ggatcaaatc tgtcgatcct tcccgcccgg tgcagtatga aggcggcgga gccgacacca
     1501 cggccaccga tattatttgc ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg
     1561 ctgtgccgaa atggtccatc aaaaaatggc tttcgctacc tggagagacg cgcccgctga
     1621 tcctttgcga atacgcccac gcgatgggta acagtcttgg cggtttcgct aaatactggc
     1681 aggcgtttcg tcagtatccc cgtttacagg gcggcttcgt ctgggactgg gtggatcagt
     1741 cgctgattaa atatgatgaa aacggcaacc cgtggtcggc ttacggcggt gattttggcg
     1801 atacgccgaa cgatcgccag ttctgtatga acggtctggt ctttgccgac cgcacgccgc
     1861 atccagcgct gacggaagca aaacaccagc agcagttttt ccagttccgt ttatccgggc
     1921 aaaccatcga agtgaccagc gaatacctgt tccgtcatag cgataacgag ctcctgcact
     1981 ggatggtggc gctggatggt aagccgctgg caagcggtga agtgcctctg gatgtcgctc
     2041 cacaaggtaa acagttgatt gaactgcctg aactaccgca gccggagagc gccgggcaac
     2101 tctggctcac agtacgcgta gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca
     2161 tcagcgcctg gcagcagtgg cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt
     2221 cccacgccat cccgcatctg accaccagcg aaatggattt ttgcatcgag ctgggtaata
     2281 agcgttggca atttaaccgc cagtcaggct ttctttcaca gatgtggatt ggcgataaaa
     2341 aacaactgct gacgccgctg cgcgatcagt tcacccgtgc accgctggat aacgacattg
     2401 gcgtaagtga agcgacccgc attgacccta acgcctgggt cgaacgctgg aaggcggcgg
     2461 gccattacca ggccgaagca gcgttgttgc agtgcacggc agatacactt gctgatgcgg
     2521 tgctgattac gaccgctcac gcgtggcagc atcaggggaa aaccttattt atcagccgga
     2581 aaacctaccg gattgatggt agtggtcaaa tggcgattac cgttgatgtt gaagtggcga
     2641 gcgatacacc gcatccggcg cggattggcc tgaactgcca gctggcgcag gtagcagagc
     2701 gggtaaactg gctcggatta gggccgcaag aaaactatcc cgaccgcctt actgccgcct
     2761 gttttgaccg ctgggatctg ccattgtcag acatgtatac cccgtacgtc ttcccgagcg
     2821 aaaacggtct gcgctgcggg acgcgcgaat tgaattatgg cccacaccag tggcgcggcg
     2881 acttccagtt caacatcagc cgctacagtc aacagcaact gatggaaacc agccatcgcc
     2941 atctgctgca cgcggaagaa ggcacatggc tgaatatcga cggtttccat atggggattg
     3001 gtggcgacga ctcctggagc ccgtcagtat cggcggaatt ccagctgagc gccggtcgct
     3061 accattacca gttggtctgg tgtcaaaaat aatcatgtaa ttagttatgt cacgcttaca
     3121 ttcacgccct ccccccacat ccgctctaac cgaaaaggaa ggagttagac aacctgaagt
     3181 ctaggtccct atttattttt ttatagttat gttagtatta agaacgttat ttatatttca
     3241 aatttttctt ttttttctgt acagacgcgt gtacgcatgt aacattatac tgaaaacctt
     3301 gcttgagaag gttttgggac gctcgaaggc tttaatttgc ggcccccggc tttccccgtc
     3361 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     3421 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     3481 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     3541 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     3601 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     3661 taacgtttac aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     3721 ccgcagggta ataactgata taattaaatt gaagctctaa tttgtgagtt tagtatacat
     3781 gcatttactt ataatacagt tttttagttt tgctggccgc atcttctcaa atatgcttcc
     3841 cagcctgctt ttctgtaacg ttcaccctct accttagcat cccttccctt tgcaaatagt
     3901 cctcttccaa caataataat gtcagatcct gtagagacca catcatccac ggttctatac
     3961 tgttgaccca atgcgtctcc cttgtcatct aaacccacac cgggtgtcat aatcaaccaa
     4021 tcgtaacctt catctcttcc acccatgtct ctttgagcaa taaagccgat aacaaaatct
     4081 ttgtcgctct tcgcaatgtc aacagtaccc ttagtatatt ctccagtaga tagggagccc
     4141 ttgcatgaca attctgctaa catcaaaagg cctctaggtt cctttgttac ttcttctgcc
     4201 gcctgcttca aaccgctaac aatacctggg cccaccacac cgtgtgcatt cgtaatgtct
     4261 gcccattctg ctattctgta tacacccgca gagtactgca atttgactgt attaccaatg
     4321 tcagcaaatt ttctgtcttc gaagagtaaa aaattgtact tggcggataa tgcctttagc
     4381 ggcttaactg tgccctccat ggaaaaatca gtcaagatat ccacatgtgt ttttagtaaa
     4441 caaattttgg gacctaatgc ttcaactaac tccagtaatt ccttggtggt acgaacatcc
     4501 aatgaagcac acaagtttgt ttgcttttcg tgcatgatat taaatagctt ggcagcaaca
     4561 ggactaggat gagtagcagc acgttcctta tatgtagctt tcgacatgat ttatcttcgt
     4621 ttcctgcagg tttttgttct gtgcagttgg gttaagaata ctgggcaatt tcatgtttct
     4681 tcaacactac atatgcgtat atataccaat ctaagtctgt gctccttcct tcgttcttcc
     4741 ttctgttcgg agattaccga atcaaaaaaa tttcaaagaa accgaaatca aaaaaaagaa
     4801 taaaaaaaaa atgatgaatt gaattgaaaa gcgtggtgca ctctcagtac aatctgctct
     4861 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     4921 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     4981 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     5041 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     5101 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     5161 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     5221 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     5281 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     5341 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     5401 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     5461 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     5521 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     5581 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     5641 ggaccgaagg agctaaccgc tttttttcac aacatggggg atcatgtaac tcgccttgat
     5701 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     5761 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     5821 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     5881 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     5941 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     6001 acgggcagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     6061 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     6121 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     6181 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     6241 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     6301 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     6361 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     6421 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6481 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6541 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6601 cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt
     6661 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6721 acgagggagc ttccaggggg gaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6781 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggccgagcct atggaaaaac
     6841 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     6901 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     6961 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     7021 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     7081 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     7141 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     7201 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagctcgaa
     7261 attaaccctc actaaaggga acaaaagctg gtaccgggcc ccccctcgag gtcgacggta
     7321 tcgataagct tgatatcgaa ttcctgcagc ccgggggatc cgagctcatt tggcgagcgt
     7381 tggttggtgg atcaagccca cgcgtaggca atcctcgagc agatccgcca ggcgtgtata
     7441 tatagcgtgg atggccaggc aactttagtg ctgacacata caggcatata tatatgtgtg
     7501 cgacgacaca tgatcatatg gcatgcatgt gctctgtatg tatataaaac tcttgttttc
     7561 ttcttttctc taaatattct ttccttatac attaggacct ttgcagcata aattactata
     7621 cttctataga cacgcaaaca caaatacaca cactaatc
//
