pCtB1 vector (V008125)

LOCUS       40924_13755        5457 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Yeast dual vector pCtB1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5457)
  AUTHORS   Lee WY, Chung S-C., Song J-Y., Cho KM.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2014) BioMaterial Lab, Samsung Advanced Institute 
            of Technology, 130 Samsung-ro, Suwon 443803, Republic of Korea
REFERENCE   2  (bases 1 to 5457)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5457)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (02-JUN-2014) BioMaterial Lab, Samsung Advanced Institute of 
            Technology, 130 Samsung-ro, Suwon 443803, Republic of Korea"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      134..1298
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     primer_bind     1356..1372
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1379..1397
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(1406..1513)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     CDS             1523..1549
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     regulatory      1553..1799
                     /note="TPS1 terminator; interior"
                     /regulatory_class="terminator"
     promoter        1821..2036
                     /label=URA3 promoter
     CDS             2037..2837
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     terminator      2841..3088
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     regulatory      3089..3301
                     /note="TPS1 terminator; lateral"
                     /regulatory_class="terminator"
     promoter        complement(3315..3333)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3354..3370)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3378..3394)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3402..3432)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3447..3468)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3756..4344)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4517..5305)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(5330..5434)
                     /label=AmpR promoter