LOCUS       40924_13755        5457 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Yeast dual vector pCtB1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5457)
  AUTHORS   Lee WY, Chung S-C., Song J-Y., Cho KM.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2014) BioMaterial Lab, Samsung Advanced Institute 
            of Technology, 130 Samsung-ro, Suwon 443803, Republic of Korea
REFERENCE   2  (bases 1 to 5457)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5457)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (02-JUN-2014) BioMaterial Lab, Samsung Advanced Institute of 
            Technology, 130 Samsung-ro, Suwon 443803, Republic of Korea"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      134..1298
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     primer_bind     1356..1372
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1379..1397
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(1406..1513)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     CDS             1523..1549
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     regulatory      1553..1799
                     /note="TPS1 terminator; interior"
                     /regulatory_class="terminator"
     promoter        1821..2036
                     /label=URA3 promoter
     CDS             2037..2837
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     terminator      2841..3088
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     regulatory      3089..3301
                     /note="TPS1 terminator; lateral"
                     /regulatory_class="terminator"
     promoter        complement(3315..3333)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3354..3370)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3378..3394)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3402..3432)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3447..3468)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3756..4344)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4517..5305)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(5330..5434)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtacgcatt taagcataaa cacgcactat gccgttcttc tcatgtatat
      181 atatatacag gcaacacgca gatataggtg cgacgtgaac agtgagctgt atgtgcgcag
      241 ctcgcgttgc attttcggaa gcgctcgttt tcggaaacgc tttgaagttc ctattccgaa
      301 gttcctattc tctagctaga aagtatagga acttcagagc gcttttgaaa accaaaagcg
      361 ctctgaagac gcactttcaa aaaaccaaaa acgcaccgga ctgtaacgag ctactaaaat
      421 attgcgaata ccgcttccac aaacattgct caaaagtatc tctttgctat atatctctgt
      481 gctatatccc tatataacct acccatccac ctttcgctcc ttgaacttgc atctaaactc
      541 gacctctaca ttttttatgt ttatctctag tattactctt tagacaaaaa aattgtagta
      601 agaactattc atagagtgaa tcgaaaacaa tacgaaaatg taaacatttc ctatacgtag
      661 tatatagaga caaaatagaa gaaaccgttc ataattttct gaccaatgaa gaatcatcaa
      721 cgctatcact ttctgttcac aaagtatgcg caatccacat cggtatagaa tataatcggg
      781 gatgccttta tcttgaaaaa atgcacccgc agcttcgcta gtaatcagta aacgcgggaa
      841 gtggagtcag gcttttttta tggaagagaa aatagacacc aaagtagcct tcttctaacc
      901 ttaacggacc tacagtgcaa aaagttatca agagactgca ttatagagcg cacaaaggag
      961 aaaaaaagta atctaagatg ctttgttaga aaaatagcgc tctcgggatg catttttgta
     1021 gaacaaaaaa gaagtataga ttctttgttg gtaaaatagc gctctcgcgt tgcatttctg
     1081 ttctgtaaaa atgcagctca gattctttgt ttgaaaaatt agcgctctcg cgttgcattt
     1141 ttgttttaca aaaatgaagc acagattctt cgttggtaaa atagcgcttt cgcgttgcat
     1201 ttctgttctg taaaaatgca gctcagattc tttgtttgaa aaattagcgc tctcgcgttg
     1261 catttttgtt ctacaaaatg aagcacagat gcttcgttaa tgtgctgcaa ggcgattaag
     1321 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattgta
     1381 atacgactca ctatagggcg aattgggtac cgggcccccc ctcgaggtcg acggtatcga
     1441 taagcttgat atcgaattcc tgcagcccgg gggatccact agttctagag cggccgccac
     1501 cgcggtggag ctcggttctg cttatcctta cgacgtgcct gactacgcct gaacccgatg
     1561 caaatgagac gatcgtctat tcctggtccg gttttctctg ccctctcttc tattcacttt
     1621 ttttatactt tatataaaat tatataaatg acataactga aacgccacac gtcctctcct
     1681 attcgttaac gcctgtctgt agcgctgtta ctgaagctgc gcaagtagtt ttttcaccgt
     1741 ataggccctc tttttctctc tctttctttc tctcccgcgc tgatctcttc ttcgaaacac
     1801 agagtgcacc ataccacctt ttcaattcat catttttttt ttattctttt ttttgatttc
     1861 ggtttccttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
     1921 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
     1981 cagtattctt aacccaactg cacagaacaa aaacctccag gaaacgaaga taaatcatgt
     2041 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
     2101 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
     2161 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
     2221 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
     2281 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
     2341 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
     2401 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
     2461 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
     2521 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
     2581 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
     2641 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
     2701 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
     2761 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
     2821 gatgcggcca gcaaaactaa tcatgtaatt agttatgtca cgcttacatt cacgccctcc
     2881 ccccacatcc gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat
     2941 ttattttttt atagttatgt tagtattaag aacgttattt atatttcaaa tttttctttt
     3001 ttttctgtac agacgcgtgt acgcatgtaa cattatactg aaaaccttgc ttgagaaggt
     3061 tttgggacgc tcgaaggctt taatttgcgt ctgtagcgct gttactgaag ctgcgcaagt
     3121 agttttttca ccgtataggc cctctttttc tctctctttc tttctctccc gcgctgatct
     3181 cttcttcgaa acatcatgaa taaaaagaaa aaggaaatca agaaaaaaaa gccataattt
     3241 atcccacatt tttttttatt gtcgctgttc acaccgcata acgaagatat tggctagcta
     3301 accagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc atggtcatag
     3361 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     3421 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     3481 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     3541 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     3601 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     3661 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     3721 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     3781 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     3841 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     3901 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     3961 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     4021 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     4081 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     4141 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     4201 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     4261 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     4321 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     4381 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     4441 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     4501 acttggtctg acatcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat
     4561 cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt
     4621 cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc
     4681 cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat
     4741 cgccatgggt cacgacgaga tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca
     4801 gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg
     4861 cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg
     4921 tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg
     4981 caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt
     5041 cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca
     5101 gccacgatag ccgcgctgcc tcgtcttgca gttcattcag ggcaccggac aggtcggtct
     5161 tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc
     5221 cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac
     5281 ctgcgtgcaa tccatcttgt tcaattcgag tgcattcaac atcagccata ctcttccttt
     5341 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     5401 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccac
//
