pCRB_mpt0 vector (V008175)

LOCUS       40924_13360        9264 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pCRB_mpt0, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9264)
  AUTHORS   Yu Z, Boehm CR, Hibberd JM, Abell C, Haseloff J, Burgess SJ, 
            Reyna-Llorens I.
  TITLE     Droplet-based microfluidic analysis and screening of single plant 
            cells
  JOURNAL   PLoS ONE 13 (5), e0196810 (2018)
  PUBMED    29723275
REFERENCE   2  (bases 1 to 9264)
  AUTHORS   Boehm CR.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2017) Plant Sciences, University of Cambridge, 
            Downing Street, Cambridge, Cambridgeshire CB2 3EA, United Kingdom
REFERENCE   3  (bases 1 to 9264)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9264)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2018"; volume: "13"; issue: "5"; pages: "e0196810"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-SEP-2017) Plant Sciences, University of Cambridge, Downing 
            Street, Cambridge, Cambridgeshire CB2 3EA, United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9264
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    6..28
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     regulatory      33..1761
                     /label=MpEF1a promoter & 5'UTR
                     /note="MpEF1a promoter & 5'UTR"
                     /regulatory_class="promoter"
     promoter        1792..1838
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             1857..2879
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     polyA_signal    3113..3289
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     3295..3311
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      3427..3679
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      3724..5452
                     /label=MpEF1a promoter & 5'UTR
                     /note="MpEF1a promoter & 5'UTR"
                     /regulatory_class="promoter"
     CDS             5458..6174
                     /label=Venus
                     /note="yellow fluorescent protein (YFP) with fast and
                     efficient maturation (Nagai et al., 2002)"
     regulatory      6255..6559
                     /label=NosT
                     /note="NosT"
                     /regulatory_class="terminator"
     terminator      6272..6524
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(6572..6588)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6649..6673
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(6764..7352)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      8702..9137
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"