LOCUS       40924_13360        9264 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pCRB_mpt0, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9264)
  AUTHORS   Yu Z, Boehm CR, Hibberd JM, Abell C, Haseloff J, Burgess SJ, 
            Reyna-Llorens I.
  TITLE     Droplet-based microfluidic analysis and screening of single plant 
            cells
  JOURNAL   PLoS ONE 13 (5), e0196810 (2018)
  PUBMED    29723275
REFERENCE   2  (bases 1 to 9264)
  AUTHORS   Boehm CR.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2017) Plant Sciences, University of Cambridge, 
            Downing Street, Cambridge, Cambridgeshire CB2 3EA, United Kingdom
REFERENCE   3  (bases 1 to 9264)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9264)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2018"; volume: "13"; issue: "5"; pages: "e0196810"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-SEP-2017) Plant Sciences, University of Cambridge, Downing 
            Street, Cambridge, Cambridgeshire CB2 3EA, United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9264
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    6..28
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     regulatory      33..1761
                     /label=MpEF1a promoter & 5'UTR
                     /note="MpEF1a promoter & 5'UTR"
                     /regulatory_class="promoter"
     promoter        1792..1838
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             1857..2879
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     polyA_signal    3113..3289
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     3295..3311
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      3427..3679
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      3724..5452
                     /label=MpEF1a promoter & 5'UTR
                     /note="MpEF1a promoter & 5'UTR"
                     /regulatory_class="promoter"
     CDS             5458..6174
                     /label=Venus
                     /note="yellow fluorescent protein (YFP) with fast and
                     efficient maturation (Nagai et al., 2002)"
     regulatory      6255..6559
                     /label=NosT
                     /note="NosT"
                     /regulatory_class="terminator"
     terminator      6272..6524
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(6572..6588)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6649..6673
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(6764..7352)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      8702..9137
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 gatcttggca ggatatattg tggtgtaacg ttcaaatgag tcacacacat tgttgagaga
       61 catatcaaaa taactcaatt gtaaggtaag ttgatagatt tcccagttaa atgcattatt
      121 ttaacaatat ttgtatctca aattttcaaa atcatatcaa atttgcagat cttgatgatg
      181 attcacaact ctgtcttcga tttttgattg ttaacgggta tcgatatttg ggtaacttaa
      241 aaagaatttg ctaaagatct taaaaaacag attaaaaaaa cctttcaaaa tagctaatcc
      301 tttaaatccg tttcacaata taatgctcat tgatgataat cgaaaagatt ccattttaaa
      361 gtgcatataa attgcttaat tatctctttc tgtctcgtaa tattttgttc tctcctattg
      421 gatcggaccc gcatcttctc gatcagttca ctatactaat atttggtcaa cttcaagaga
      481 gattagaact tcgttctcca aagtgtaaag gccgacaagc agaaaaggaa acttcaatca
      541 gaagccttct aggttttccc gcgtcccaaa aagcgaaggg aggacgggga ggacggtgag
      601 gccagaagaa gaaagcaaca gtaaaatctc atgtgattgc gtgcatgtca tggatctatg
      661 ggatgctagt ccaccgtttg gaagtatgtg cagaaggaga aaggcgcggg aggccaaaag
      721 cgacttcggc cacctctcta ctttctagaa agtttgctcg cagcttgtaa taagcgcgga
      781 aaaggtactc gaaaaaccgg agcaatcggt gtggtatcag cagtcgggtt cgctaaacct
      841 acgtcttctt attttggaat gttaaattga aagggcgtgc cggccctaag cacggacgat
      901 gtgacagacg gaggagtgtg agcaattcgg atgacgatgc tggccggcag tcacgacaaa
      961 gagtccgaag gggcatggac agggcaatat ataaatggaa aagaggcttt gaatattcct
     1021 aacccttcgt cgtcggcctc ccaacttgcc ctcgcatgct gaattggccg cctatctgcg
     1081 aatggccggc cgccgaccct aagtcgcaat aacttgaacg gagccgatgc aaagtcctta
     1141 tcgaacgctc gttattataa acggggcaga cctaggtatt taattatatc aaaaccctcc
     1201 gcaatttcgc taatcattcc ctaatttctt ctactttggc gtctagtgct gtggtcgcgc
     1261 ttgctctcaa agctcgctgg gaggcagtgg agcgtctggc ttaaaatcga tcgccgcaac
     1321 cctagagttc ctttcacttc tttctctctt gcgtggagag catcgacatc gaagagctgc
     1381 atcttgtctt ctggtatgcg ccttgtgccc tcgtctctgt acgtcgttac gattgctcga
     1441 tattgtgatt gtctagacct gaaacccgac tggagctttt acgtggtgtc gattggggcc
     1501 tcgagcgagt gattttttag gttcgtttta gccaagtaca tcccactttg gtcagtcctg
     1561 ttatctcggt aaggtgttca gttcggtctc tggtgcggag gtgcctcgta agtttcctgc
     1621 atgatagatt tgtctatcct tgacttgaag aggagtcgaa ggtcagcgag gttggctgtg
     1681 tgctatggac gtcggtgctc cttggttccg tgcttctatg ggtgttgcct caacagtatt
     1741 gatgtgcctg cagaaaggtt gtcaccatga cgcacaatcc cactatcctt cgcaagaccc
     1801 ttcctctata taaggaagtt catttcattt ggagaggaca gcccaagctg atccctatga
     1861 aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg
     1921 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag
     1981 gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt
     2041 atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg
     2101 agttcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag
     2161 acctgcctga aaccgaactg cccgctgttc ttcagccggt cgcggaggct atggatgcga
     2221 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg
     2281 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact
     2341 ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga
     2401 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca
     2461 acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt
     2521 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta
     2581 tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccacgcc
     2641 tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca
     2701 atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg
     2761 ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg
     2821 tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat
     2881 agagtagatg ccgaccgaac aagagctgat ttcgagaacg cctcagccag caactcgcgc
     2941 gagcctagca aggcaaatgc gagagaacgg ccttacgctt ggtggcacag ttctcgtcca
     3001 cagttcgcta agctcgctcg gctggtcgcg ggagggccgg tcgcaatgat tcaggaatta
     3061 attcggtacg ctgaaatcac cagtctctct ctacaaatct atctctctct attttctcca
     3121 taaataatgt gtgagtagtt tcccgataag ggaaattagg gttcttatag ggtttcgctc
     3181 atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca
     3241 ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcg atcacaggaa
     3301 acagctatga ccatgattac gcccgggctt gacctgaagc agatcaacaa ctggttcatc
     3361 aaccagcgga agcggcactg gaagccatcc gagtaacatc aaaaatgaat ctgcccgaat
     3421 ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
     3481 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
     3541 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
     3601 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
     3661 tcatctatgt tactagatcg ggaattcata gtttggccgc gaattcgccc ttgatatcca
     3721 cgtcaaatga gtcacacaca ttgttgagag acatatcaaa ataactcaat tgtaaggtaa
     3781 gttgatagat ttcccagtta aatgcattat tttaacaata tttgtatctc aaattttcaa
     3841 aatcatatca aatttgcaga tcttgatgat gattcacaac tctgtcttcg atttttgatt
     3901 gttaacgggt atcgatattt gggtaactta aaaagaattt gctaaagatc ttaaaaaaca
     3961 gattaaaaaa acctttcaaa atagctaatc ctttaaatcc gtttcacaat ataatgctca
     4021 ttgatgataa tcgaaaagat tccattttaa agtgcatata aattgcttaa ttatctcttt
     4081 ctgtctcgta atattttgtt ctctcctatt ggatcggacc cgcatcttct cgatcagttc
     4141 actatactaa tatttggtca acttcaagag agattagaac ttcgttctcc aaagtgtaaa
     4201 ggccgacaag cagaaaagga aacttcaatc agaagccttc taggttttcc cgcgtcccaa
     4261 aaagcgaagg gaggacgggg aggacggtga ggccagaaga agaaagcaac agtaaaatct
     4321 catgtgattg cgtgcatgtc atggatctat gggatgctag tccaccgttt ggaagtatgt
     4381 gcagaaggag aaaggcgcgg gaggccaaaa gcgacttcgg ccacctctct actttctaga
     4441 aagtttgctc gcagcttgta ataagcgcgg aaaaggtact cgaaaaaccg gagcaatcgg
     4501 tgtggtatca gcagtcgggt tcgctaaacc tacgtcttct tattttggaa tgttaaattg
     4561 aaagggcgtg ccggccctaa gcacggacga tgtgacagac ggaggagtgt gagcaattcg
     4621 gatgacgatg ctggccggca gtcacgacaa agagtccgaa ggggcatgga cagggcaata
     4681 tataaatgga aaagaggctt tgaatattcc taacccttcg tcgtcggcct cccaacttgc
     4741 cctcgcatgc tgaattggcc gcctatctgc gaatggccgg ccgccgaccc taagtcgcaa
     4801 taacttgaac ggagccgatg caaagtcctt atcgaacgct cgttattata aacggggcag
     4861 acctaggtat ttaattatat caaaaccctc cgcaatttcg ctaatcattc cctaatttct
     4921 tctactttgg cgtctagtgc tgtggtcgcg cttgctctca aagctcgctg ggaggcagtg
     4981 gagcgtctgg cttaaaatcg atcgccgcaa ccctagagtt cctttcactt ctttctctct
     5041 tgcgtggaga gcatcgacat cgaagagctg catcttgtct tctggtatgc gccttgtgcc
     5101 ctcgtctctg tacgtcgtta cgattgctcg atattgtgat tgtctagacc tgaaacccga
     5161 ctggagcttt tacgtggtgt cgattggggc ctcgagcgag tgatttttta ggttcgtttt
     5221 agccaagtac atcccacttt ggtcagtcct gttatctcgg taaggtgttc agttcggtct
     5281 ctggtgcgga ggtgcctcgt aagtttcctg catgatagat ttgtctatcc ttgacttgaa
     5341 gaggagtcga aggtcagcga ggttggctgt gtgctatgga cgtcggtgct ccttggttcc
     5401 gtgcttctat gggtgttgcc tcaacagtat tgatgtgcct gcagaaaggt tgtcaccatg
     5461 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     5521 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     5581 aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     5641 gtgaccaccc tgggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag
     5701 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     5761 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     5821 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     5881 ctggagtaca actacaacag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc
     5941 atcaaggcca acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac
     6001 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     6061 ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     6121 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaataa
     6181 agggccgcca ctccaccggc gcctaagagc tcgaatttcc ccgatcggcg tcttggattc
     6241 gcttgaattg ctaccgagct cgaatttccc cgatcgttca aacatttggc aataaagttt
     6301 cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta
     6361 cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat
     6421 gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa
     6481 ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat tctccagctt
     6541 ttgttccctt tagtgagggg ggaattaatt cactggccgt cgttttacaa cgtcgtgact
     6601 gggaaaaccc tggcgttagg gataacgcag gaaagaacat gaaggccttg acaggatata
     6661 ttggcgggta aactaagtcg ctgtatgtgt ttgtttgaga tctcatgtga gcaaaaggcc
     6721 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6781 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6841 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     6901 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     6961 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7021 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7081 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7141 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7201 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga agaagagttg
     7261 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7321 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7381 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7441 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7501 atgtgtaaca ttggtctagt gattagaaaa actcatcgag catcaaatga aactgcaatt
     7561 tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag
     7621 aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga
     7681 ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg
     7741 agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagttta tgcatttctt
     7801 tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca
     7861 aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag
     7921 gacaattaca aacaggaatc gaatgcaacc ggcgcagcct gagcgagacg aaatacgcga
     7981 tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc
     8041 agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt
     8101 ttccctggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg
     8161 atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca
     8221 acattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca
     8281 tacaatcggt agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca
     8341 tataaatcag catccatgtt ggaatttaat cgcggccttg agcaagacgt ttcccgttga
     8401 atatggctca taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat
     8461 gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtggctt
     8521 tgttgaataa atcgaacttt tgctgagttg aaggatcaga tcacgcatct tcccgacaac
     8581 gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca actggtccac ctacaacaaa
     8641 gctctcatca accgtggctc cctcactttc tggctggatg atggggcgat tcaggcgatc
     8701 cccatccaac agcccgccgt cgagcgggct tttttatccc cggaagcctg tggatagagg
     8761 gtagttatcc acgtgaaacc gctaatgccc cgcaaagcct tgattcacgg ggctttccgg
     8821 cccgctccaa aaactatcca cgtgaaatcg ctaatcaggg tacgtgaaat cgctaatcgg
     8881 agtacgtgaa atcgctaata aggtcacgtg aaatcgctaa tcaaaaaggc acgtgagaac
     8941 gctaatagcc ctttcagatc aacagcttgc aaacacccct cgctccggca agtagttaca
     9001 gcaagtagta tgttcaatta gcttttcaat tatgaatata tatatcaatt attggtcgcc
     9061 cttggcttgt ggacaatgcg ctacgcgcac cggctccgcc cgtggacaac cgcaagcggt
     9121 tgcccaccgt cgagcgccag cgcctttgcc cacaacccgg cggccggccg caacagatcg
     9181 ttttataaat tttttttttt gaaaaagaaa aagcccgaaa ggcggcaacc tctcgggctt
     9241 ctggatttcc gatccccgga atta
//
