Basic Vector Information
- Vector Name:
- pLV-mCherry
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7413 bp
- Type:
- Mammalian Expression, Lentiviral
- Replication origin:
- ori
- Promoter:
- RSV
- Cloning Method:
- Restriction Enzyme
- 5' Primer:
- CMV fwd
- 3' Primer:
- WPRE-R
pLV-mCherry vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLV-mCherry vector Sequence
LOCUS V012576 7413 bp DNA circular SYN 13-MAY-2021
DEFINITION Exported.
ACCESSION V012576
VERSION V012576
KEYWORDS pLV-mCherry.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 7413)
TITLE Tsoulfas Lab plasmids
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7413)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 7413)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7413
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(65..82)
/label="L4440"
/note="L4440 vector, forward primer"
rep_origin complement(236..824)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(998..1855)
/label="AmpR"
/note="beta-lactamase"
promoter complement(1856..1960)
/label="AmpR promoter"
rep_origin complement(1986..2441)
/direction=LEFT
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 2583..2599
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter 2609..2627
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
rep_origin complement(2648..2783)
/direction=LEFT
/label="SV40 ori"
/note="SV40 origin of replication"
polyA_signal complement(2810..2944)
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
LTR complement(3016..3249)
/label="3' LTR (Delta-U3)"
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
misc_feature complement(3336..3924)
/label="WPRE"
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(3934..4641)
/label="mCherry"
/note="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
primer_bind complement(4712..4736)
/label="LNCX"
/note="Human CMV promoter, forward primer"
promoter complement(4733..4936)
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
enhancer complement(4937..5240)
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
misc_feature complement(5263..5379)
/label="cPPT/CTS"
/note="central polypurine tract and central termination
sequence of HIV-1"
CDS complement(5456..5497)
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
/label="Protein Tat"
CDS complement(5646..5690)
/label="gp41 peptide"
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
misc_feature complement(5875..6108)
/label="RRE"
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature complement(6601..6726)
/label="HIV-1 Psi"
/note="packaging signal of human immunodeficiency virus
type 1"
LTR 6773..6953
/label="5' LTR (truncated)"
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
promoter complement(6954..7180)
/label="RSV promoter"
/note="Rous sarcoma virus enhancer/promoter"
promoter complement(7208..7226)
/label="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(7247..7263)
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(7271..7287)
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7295..7325)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(7340..7361)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
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