LOCUS       V012576                 7413 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V012576
VERSION     V012576
KEYWORDS    pLV-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7413)
  TITLE     Tsoulfas Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7413)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7413)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(65..82)
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(236..824)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(998..1855)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1856..1960)
                     /label="AmpR promoter"
     rep_origin      complement(1986..2441)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     2583..2599
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2609..2627
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2648..2783)
                     /direction=LEFT
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     polyA_signal    complement(2810..2944)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     LTR             complement(3016..3249)
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    complement(3336..3924)
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     CDS             complement(3934..4641)
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     primer_bind     complement(4712..4736)
                     /label="LNCX"
                     /note="Human CMV promoter, forward primer"
     promoter        complement(4733..4936)
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(4937..5240)
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    complement(5263..5379)
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     CDS             complement(5456..5497)
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     CDS             complement(5646..5690)
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     misc_feature    complement(5875..6108)
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     misc_feature    complement(6601..6726)
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     LTR             6773..6953
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6954..7180)
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     promoter        complement(7208..7226)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7247..7263)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7271..7287)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7295..7325)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7340..7361)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
       61 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
      121 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
      181 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
      241 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
      301 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
      361 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
      421 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
      481 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
      541 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
      601 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
      661 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
      721 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
      781 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
      841 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
      901 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
      961 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     1021 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     1081 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     1141 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     1201 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     1261 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     1321 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     1381 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     1441 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     1501 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     1561 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     1621 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     1681 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     1741 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     1801 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     1861 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     1921 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     1981 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     2041 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     2101 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     2161 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     2221 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     2281 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     2341 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     2401 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
     2461 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     2521 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     2581 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac
     2641 gtccctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg
     2701 gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa
     2761 ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggatagctag agccagacat
     2821 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     2881 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     2941 agttcctctc actctctgat attcatttct ttgcaagtta taaatactga ataataagat
     3001 gacatgaact actactgcta gagattttcc acactgacta aaagggtctg agggatctct
     3061 agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt
     3121 attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt
     3181 ctaaccagag agacccagta caagcaaaaa gcagatcttg tcttcgttgg gagtgaatta
     3241 gcccttccag tccccccttt tcttttaaaa agtggctaag atctacagct gccttgtaag
     3301 tcattggtct taaaggtacc gagctcgaat tccaggcggg gaggcggccc aaagggagat
     3361 ccgactcgtc tgagggcgaa ggcgaagacg cggaagaggc cgcagagccg gcagcaggcc
     3421 gcgggaagga aggtccgctg gattgagggc cgaagggacg tagcagaagg acgtcccgcg
     3481 cagaatccag gtggcaacac aggcgagcag ccatggaaag gacgtcagct tccccgacaa
     3541 caccacggaa ttgtcagtgc ccaacagccg agcccctgtc cagcagcggg caaggcaggc
     3601 ggcgatgagt tccgccgtgg caatagggag ggggaaagcg aaagtcccgg aaaggagctg
     3661 acaggtggtg gcaatgcccc aaccagtggg ggttgcgtca gcaaacacag tgcacaccac
     3721 gccacgttgc ctgacaacgg gccacaactc ctcataaaga gacagcaacc aggatttata
     3781 caaggaggag aaaatgaaag ccatacggga agcaatagca tgatacaaag gcattaaagc
     3841 agcgtatcca catagcgtaa aaggagcaac atagttaaga ataccagtca atctttcaca
     3901 aattttgtaa tccagaggtt gattgtcgac ttacttgtac agctcgtcca tgccgccggt
     3961 ggagtggcgg ccctcggcgc gttcgtactg ttccacgatg gtgtagtcct cgttgtggga
     4021 ggtgatgtcc aacttgatgt tgacgttgta ggcgccgggc agctgcacgg gcttcttggc
     4081 cttgtaggtg gtcttgacct cagcgtcgta gtggccgccg tccttcagct tcagcctctg
     4141 cttgatctcg cccttcaggg cgccgtcctc ggggtacatc cgctcggagg aggcctccca
     4201 gcccatggtc ttcttctgca ttacggggcc gtcggagggg aagttggtgc cgcgcagctt
     4261 caccttgtag atgaactcgc cgtcctgcag ggaggagtcc tgggtcacgg tcaccacgcc
     4321 gccgtcctcg aagttcatca cgcgctccca cttgaagccc tcggggaagg acagcttcaa
     4381 gtagtcgggg atgtcggcgg ggtgcttcac gtaggccttg gagccgtaca tgaactgagg
     4441 ggacaggatg tcccaggcga agggcagggg gccacccttg gtcaccttca gcttggcggt
     4501 ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt
     4561 cacggagccc tccatgtgca ccttgaagcg catgaactcc ttgatgatgg ccatgttatc
     4621 ctcctcgccc ttgctcacca tggtggcggc ggatcctcta gagtcggtgt cttctatgga
     4681 ggtcaaaaca gcgtggatgg cgtctccagg cgatctgacg gttcactaaa cgagctctgc
     4741 ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt
     4801 tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa
     4861 tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact
     4921 gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag
     4981 gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga
     5041 cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt
     5101 accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa
     5161 catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat
     5221 ttaccgtaag ttatgtaacg cggaactccc aagcttatcg ataaaatttt gaattttgta
     5281 atttgttttt gtaattcttt agtttgtatg tctgttgcta ttatgtctac tattctttcc
     5341 cctgcactgt accccccaat cccccctttt cttttaaaag ttaaccgata ccgtcgagat
     5401 ccgttcacta atcgaatgga tctgtctctg tctctctctc caccttcttc ttctattcct
     5461 tcgggcctgt cgggtcccct cggggttggg aggtgggtct gaaacgataa tggtgaatat
     5521 ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa acctaccaag
     5581 cctcctacta tcattatgaa taattttata taccacagcc aatttgttat gttaaaccaa
     5641 ttccacaaac ttgcccattt atctaattcc aataattctt gttcattctt ttcttgctgg
     5701 ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc
     5761 cactccatcc aggtcgtgtg attccaaatc tgttccagag atttattact ccaactagca
     5821 ttccaaggca cagcagtggt gcaaatgagt tttccagagc aaccccaaat ccccaggagc
     5881 tgttgatcct ttaggtatct ttccacagcc aggattcttg cctggagctg cttgatgccc
     5941 cagactgtga gttgcaacag atgctgttgc gcctcaatag ccctcagcaa attgttctgc
     6001 tgctgcacta taccagacaa taattgtctg gcctgtaccg tcagcgtcat tgacgctgcg
     6061 cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca aagctcctat tcccactgct
     6121 cttttttctc tctgcaccac tcttctcttt gccttggtgg gtgctactcc taatggttca
     6181 atttttacta ctttatattt atataattca cttctccaat tgtccctcat atctcctcct
     6241 ccaggtctga agatcagcgg ccgcttgctg tgcggtggtc ttacttttgt tttgctcttc
     6301 ctctatcttg tctaaagctt ccttggtgtc ttttatctct atcctttgat gcacacaata
     6361 gagggttgct actgtattat ataatgatct aagttcttct gatcctgtct gaagggatgg
     6421 ttgtagctgt cccagtattt gtctacagcc ttctgatgtt tctaacaggc caggattaac
     6481 tgcgaatcgt tctagctccc tgcttgccca tactatatgt tttaatttat attttttctt
     6541 tccccctggc cttaaccgaa ttttttccca tcgcgatcta attctccccc gcttaatact
     6601 gacgctctcg cacccatctc tctccttcta gcctccgcta gtcaaaattt ttggcgtact
     6661 caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag tcctgcgtcg
     6721 agagagctcc tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc actgctagag
     6781 attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga
     6841 cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc
     6901 cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga cccgtttatg
     6961 tatcgagcta ggcacttaaa tacaatatct ctgcaatgcg gcaattcagt ggttcgtcca
     7021 atccatgtca gacccgtctg ttgccttcct aataaggcac gatcgtacca ccttacttcc
     7081 accaatcggc atgcacggtg ctttttctct ccttgtaagg catgttgcta actcatcgtt
     7141 accatgttgc aagactacaa gagtattgca taagactaca ttaagcttgc agctccagct
     7201 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc
     7261 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     7321 ggaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     7381 ccgctttcca gtcgggaaac ctgtcgtgcc agc
//
