pBHRF-108-Ypet vector (V009197)

LOCUS       40924_6342       10779 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Transformation vector pBHRF-108-Ypet, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10779)
  AUTHORS   Moody LA, Kelly S, Coudert Y, Nimchuk ZL, Harrison CJ, Langdale JA.
  TITLE     Somatic hybridization provides segregating populations for the 
            identification of causative mutations in sterile mutants of the moss
            Physcomitrella patens
  JOURNAL   New Phytol. (2018) In press
  PUBMED    29498048
REFERENCE   2  (bases 1 to 10779)
  AUTHORS   Coudert Y, Nimchuk ZL, Harrison CJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2018) Department of Plant Sciences, University of 
            Oxford, South Parks Road, Oxford OX1 3RB, UK
REFERENCE   3  (bases 1 to 10779)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10779)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "New Phytol.
            (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-JAN-2018) Department of Plant Sciences, University of Oxford, 
            South Parks Road, Oxford OX1 3RB, UK"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     142..158
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    182..1293
                     /note="108 targeting sequence for ectopic integration of 
                     transgenes"
     protein_bind    1363..1396
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1599..1944
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1977..3002
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     regulatory      3024..3198
                     /label=35S terminator
                     /note="35S terminator"
                     /regulatory_class="terminator"
     promoter        3242..3260
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     terminator      complement(3293..3999)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             complement(4018..4728)
                     /label=YPet
                     /note="yellow fluorescent protein optimized for FRET with
                     CyPet (Nguyen and Daugherty, 2005)"
     misc_feature    complement(4738..5081)
                     /label=29-1
                     /note="29-1"
     promoter        complement(5098..7064)
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     protein_bind    7087..7107
                     /label=attB2
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     misc_feature    7120..8120
                     /note="108 targeting locus for ectopic integration of 
                     transgenes"
     promoter        complement(8138..8156)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8171..8187)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8195..8211
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8219..8249)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8264..8285)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8573..9161)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9335..10192)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(10193..10297)
                     /label=AmpR promoter
     rep_origin      10324..10779
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"