LOCUS       40924_6342       10779 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Transformation vector pBHRF-108-Ypet, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10779)
  AUTHORS   Moody LA, Kelly S, Coudert Y, Nimchuk ZL, Harrison CJ, Langdale JA.
  TITLE     Somatic hybridization provides segregating populations for the 
            identification of causative mutations in sterile mutants of the moss
            Physcomitrella patens
  JOURNAL   New Phytol. (2018) In press
  PUBMED    29498048
REFERENCE   2  (bases 1 to 10779)
  AUTHORS   Coudert Y, Nimchuk ZL, Harrison CJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2018) Department of Plant Sciences, University of 
            Oxford, South Parks Road, Oxford OX1 3RB, UK
REFERENCE   3  (bases 1 to 10779)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10779)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "New Phytol.
            (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-JAN-2018) Department of Plant Sciences, University of Oxford, 
            South Parks Road, Oxford OX1 3RB, UK"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     142..158
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    182..1293
                     /note="108 targeting sequence for ectopic integration of 
                     transgenes"
     protein_bind    1363..1396
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1599..1944
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1977..3002
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     regulatory      3024..3198
                     /label=35S terminator
                     /note="35S terminator"
                     /regulatory_class="terminator"
     promoter        3242..3260
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     terminator      complement(3293..3999)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             complement(4018..4728)
                     /label=YPet
                     /note="yellow fluorescent protein optimized for FRET with
                     CyPet (Nguyen and Daugherty, 2005)"
     misc_feature    complement(4738..5081)
                     /label=29-1
                     /note="29-1"
     promoter        complement(5098..7064)
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     protein_bind    7087..7107
                     /label=attB2
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     misc_feature    7120..8120
                     /note="108 targeting locus for ectopic integration of 
                     transgenes"
     promoter        complement(8138..8156)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8171..8187)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8195..8211
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8219..8249)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8264..8285)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8573..9161)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9335..10192)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(10193..10297)
                     /label=AmpR promoter
     rep_origin      10324..10779
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      121 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctaatt accctgttat
      181 ccctaggagt gtgcaaccaa ataccgaccc tgcacctatc gaagacacag cgatcatatc
      241 aaatcacata tttgacatgt caagatgcaa acttgttatc tacacaaaat tgacctaaga
      301 tataattaag cctatagagc tccatatcat aggcttactc atgaaaacat caactcacgg
      361 actgtcgtct ttcaagaaga acgttagcat gaatgttcca caataaacaa aaagaaaagt
      421 ttagaagtaa ggacagatac catcgacact ggagaaaata gccatccgta gacgatgcct
      481 cggtaacatc ttcaggagaa agactcgaat ctccccgtcg caacagcaca taggccgaca
      541 actcgcagcc tacaatggga atgtcggtcg ggaggtgcac ccgcactaca ctcagcattc
      601 ttccccgctt tcgccccctc ccttatctcc agctccgctt aagatgatac tcacgctttt
      661 gtacttcggc tctttcactt ccagacgaat ttcatgaaaa gttacatgtg cttgaggccg
      721 atcagccatc aggcgccccc tgtgcgcgcc agcgttctcc cctcaacctc gccccctctc
      781 actctttgaa aagtgtgtgt ctaaatctgg cagtggcgat ccctcgaaac ctaaaactct
      841 tgcgaaacat aagtaactta agttttcctc tacaaaactt gcaagtacga catgcaccca
      901 cacaagtgca acacggacgc ctcttgtttt ctctacattc tcatagtaga acatgagatt
      961 cctctcagaa acgcattccc tcccattcac ttaatcccct gttccacgcg tgtcagggag
     1021 tactcttgca atcataaagc gatggcttgg tcattcaaca cttggtagct tcctgcaaat
     1081 aaaccctaaa gttgtcagag cacttgagga gaagctcttc cctcactcca cctattcaca
     1141 ctacctttcc acttgagaag gacagctgct gcagcaaacc cccaaaaaaa aatagggagc
     1201 gggaggggaa caataaatgc attacctaaa ccacgcaacc atctctcatc gaacaagcct
     1261 caccgcaacg cagactcttc ctccaacacc cctgtcgact ctagaggatc ccacgtgccc
     1321 gggcgtttaa accaattgtt cgaacgtacg tcgcgactcg agataacttc gtataatgta
     1381 tgctatacga agttatggta ccgagctcga attcccatgg agtcaaagat tcaaatagag
     1441 gacctaacag aactcgccgt aaagactggc gaacagttca tacagagtct cttacgactc
     1501 aatgacaaga agaaaatctt cgtcaacatg gtggagcacg acacgcttgt ctactccaaa
     1561 aatatcaaag atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta
     1621 atatccggaa acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata
     1681 gtggaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt
     1741 gaagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     1801 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga tatctccact
     1861 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     1921 agttcatttc atttggagag gacagggtac ccggggatcc ccgggggcaa tgagatatga
     1981 aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg
     2041 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag
     2101 gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt
     2161 atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg
     2221 aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag
     2281 acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga
     2341 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg
     2401 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact
     2461 ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga
     2521 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca
     2581 acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt
     2641 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta
     2701 tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc
     2761 tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca
     2821 atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg
     2881 ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg
     2941 tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccggga tcctctagag
     3001 tcgacctgca ggcatgccgc tgaaatcacc agtctctctc tacaaatcta tctctctcta
     3061 taataatgtg tgagtagttc ccagataagg gaattagggt tcttataggg tttcgctcat
     3121 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3181 aaaatttcta attcctaaaa ccaaaatcca gtgggtaccg agctcgaatt cgcggccgca
     3241 gatttaggtg acactataga atatcactag taagctagct tgcatgccgg tcctgctgag
     3301 cctcgacatg ttgtcgcaaa attcgccctg gacccgccca acgatttgtc gtcactgtca
     3361 aggtttgacc tgcacttcat ttggggccca catacaccaa aaaaatgctg cataattctc
     3421 ggggcagcaa gtcggttacc cggccgccgt gctggaccgg gttgaatggt gcccgtaact
     3481 ttcggtagag cggacggcca atactcaact tcaaggaatc tcacccatgc gcgccggcgg
     3541 ggaaccggag ttcccttcag tgaacgttat tagttcgccg ctcggtgtgt cgtagatact
     3601 agcccctggg gccttttgaa atttgaataa gatttatgta atcagtcttt taggtttgac
     3661 cggttctgcc gcttttttta aaattggatt tgtaataata aaacgcaatt gtttgttatt
     3721 gtggcgctct atcatagatg tcgctataaa cctattcagc acaatatatt gttttcattt
     3781 taatattgta catataagta gtagggtaca atcagtaaat tgaacggaga atattattca
     3841 taaaaatacg atagtaacgg gtgatatatt cattagaatg aaccgaaacc ggcggtaagg
     3901 atctgagcta cacatgctca ggttttttac aacgtgcaca acagaattga aagcaaatat
     3961 catgcgatca taggcgtctc gcatatctca ttaaagcagg actctagagg atccttattt
     4021 gtacaattca ttcataccct cggtaatacc agcagcagtc aaaaattcta acaagaccat
     4081 gtgatcgcgc ttctcgttgg ggtctttgaa cagtttggac tggtagctca ggtagtggtt
     4141 gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag
     4201 ctgcacgccg ccgtcctcga tgttgtggcg gatcttgaag ttggccttga tgccgttctt
     4261 ctgcttgtcg gcggtgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc
     4321 caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt
     4381 gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat
     4441 ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat
     4501 gtggtcgggg tagcgggcga agcactgcac gccgtagccc agggtggtca cgagggtggg
     4561 ccagggcacg ggcagcttgc cggtggtgca gagcagcttc agggtcagct tgccgtaggt
     4621 ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag
     4681 ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttggctg cagccatgga
     4741 tcgggctgcc gcagcggcag cagccgcagc aggtttggtg aggacataaa tggcgtatat
     4801 gatcccagga atatacccaa gtagcgtcaa aaccaaacat atccaaaact caacctgcaa
     4861 caaaattcat aatttcaact tttaatttct agtgtggata aatgtaaaaa aagaaccata
     4921 tttgactaaa gtataagatt aattaaccaa aataaaactc agtctaaata tgagaggaaa
     4981 gataaatacc ccgcaaccaa atctgagaaa gacaccgagt ggaggcaaga ggatggcgat
     5041 aataatatca acgaaagtag ctgtactcat ggatccaagc ttatcgattt cgaacccaca
     5101 gggtgagcat cgacaaaaga aacagtacca agcaaataaa tagcgtatga aggcagggct
     5161 aaaaaaatcc acatatagct gctgcatatg ccatcatcca agtatatcaa gatcaaaata
     5221 attataaaac atacttgttt attataatag ataggtactc aaggttagag catatgaata
     5281 gatgctgcat atgccatcat gtatatgcat cagtaaaacc cacatcaaca tgtataccta
     5341 tcctagatcg atatttccat ccatcttaaa ctcgtaacta tgaagatgta tgacacacac
     5401 atacagttcc aaaattaata aatacaccag gtagtttgaa acagtattct actccgatct
     5461 agaacgaatg aacgaccgcc caaccacacc acatcatcac aaccaagcga acaaaaagca
     5521 tctctgtata tgcatcagta aaacccgcat caacatgtat acctatccta gatcgatatt
     5581 tccatccatc atcttcaatt cgtaactatg aatatgtatg gcacacacat acagatccaa
     5641 aattaataaa tccaccaggt agtttgaaac agaattctac tccgatctag aacgaccgcc
     5701 caaccagacc acatcatcac aaccaagaca aaaaaaagca tgaaaagatg acccgacaaa
     5761 caagtgcacg gcatatattg aaataaagga aaagggcaaa ccaaacccta tgcaacgaaa
     5821 caaaaaaaat catgaaatcg atcccgtctg cggaacggct agagccatcc caggattccc
     5881 caaagagaaa cactggcaag ttagcaatca gaacgtgtct gacgtacagg tcgcatccgt
     5941 gtacgaacgc tagcagcacg gatctaacac aaacacggat ctaacacaaa catgaacaga
     6001 agtagaacta ccgggcccta accatggacc ggaacgccga tctagagaag gtagagaggg
     6061 gggggggggg aggacgagcg gcgtaccttg aagcggaggt gccgacgggt ggatttgggg
     6121 gagatctggt tgtgtgtgtg tgcgctccga acaacacgag gttggggaaa gagggtgtgg
     6181 agggggtgtc tatttattac ggcgggcgag gaagggaaag cgaaggagcg gtgggaaagg
     6241 aatcccccgt agctgccgtg ccgtgagagg aggaggaggc cgcctgccgt gccggctcac
     6301 gtctgccgct ccgccacgca atttctggat gccgacagcg gagcaagtcc aacggtggag
     6361 cggaactctc gagaggggtc cagaggcagc gacagagatg ccgtgccgtc tgcttcgctt
     6421 ggcccgacgc gacgctgctg gttcgctggt tggtgtccgt tagactcgtc gacggcgttt
     6481 aacaggctgg cattatctac tcgaaacaag aaaaatgttt ccttagtttt tttaatttct
     6541 taaagggtat ttgtttaatt tttagtcact ttattttatt ctattttata tctaaattat
     6601 taaataaaaa aactaaaata gagttttagt tttcttaatt tagaggctaa aatagaataa
     6661 aatagatgta ctaaaaaaat tagtctataa aaaccattaa ccctaaaccc taaatggatg
     6721 tactaataaa atggatgaag tattatatag gtgaagctat ttgcaaaaaa aaaggagaac
     6781 acatgcacac taaaaagata aaactgtaga gtcctgttgt caaaatactc aattgtcctt
     6841 tagaccatgt ctaactgttc atttatatga ttctctaaaa cactgatatt attgtagtac
     6901 tatagattat attattcgta gagtaaagtt taaatatatg tataaagata gataaactgc
     6961 acttcaaaca agtgtgacaa aaaaaatatg tggtaatttt ttataactta gacatgcaat
     7021 gctcattatc tctagagagg ggcacgaccg ggtcacgctg cacggaattc gattcgaggg
     7081 ggatcaccac tttgtacaag aaagctggtt cggcgcgcct cgtaccacac cacttccagg
     7141 ctcagaagca gaatcgattc agcagtcact aaccctctct atgcgacagc cacgagaatt
     7201 acaaactggg tctgccggac ttacatatca atcaacggaa tcacgtccaa gtaaaataat
     7261 ccagcgaact tatccacata ggatctgagg ggggaacgtg aatgagccag gtctacacgc
     7321 agcatgggag agaagggggg ggggggtttt caaatagagt ccaaaattag cccaaacgag
     7381 ggagggagaa tgaatgaggc tgtcgagggc gggcggagat ggaacgaaga gtcggggggg
     7441 gggggggata gcgaaggcga agaggctctc acccacccat tgcgcttctt tgcgaactcg
     7501 accaccgaat ctctccctcc cttccctctt cctctccctc tcctctctct ctctctctcg
     7561 tatgtgtgtg tgtgtctaaa ttcgcccaat tcgtcgtccc ttccgccatc gccattgcca
     7621 tgccaatcaa tgccttagaa acgggcacta ccatggcatc cccccctttg catccttgga
     7681 ccctccggag ggagggaggg agtcaacgtt cacactccga agtcaacctg gtcgctagac
     7741 ttttggcttc ctctgtttat cttttgtctc ggtagatccc tcttggaata tatggatgga
     7801 tggatgaaat tatggatgaa tgattgaatg gcatttgtga accattcgat ccctcctgag
     7861 tgcctgtgtc cgtcagtccg tgtaactgtg actgactgtt ctatctcatc atccatcctt
     7921 tcttcatgct ctactcggac tgaactctct ctctctctct ctctctctct ctcgctctgt
     7981 gtatgtgtgt gtgtgtttgt ttattgactt ctgaacttgt catttggttc taatgtagag
     8041 tgaaagtcgt ttaaagcaga atatgcaaat cgtgcatttt aagtggtgta atgtaatgta
     8101 ttttggggtg gtgatgtcat taattaaatt taaattccct atagtgagtc gtattaaatt
     8161 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     8221 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     8281 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     8341 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     8401 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     8461 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     8521 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     8581 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     8641 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     8701 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     8761 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     8821 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     8881 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     8941 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     9001 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     9061 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     9121 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     9181 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     9241 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     9301 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     9361 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     9421 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     9481 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     9541 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     9601 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     9661 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     9721 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     9781 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     9841 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     9901 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     9961 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    10021 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    10081 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    10141 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    10201 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    10261 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    10321 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
    10381 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
    10441 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg catcccttta gggttccgat
    10501 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
    10561 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
    10621 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
    10681 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
    10741 ttaacgcgaa ttttaacaaa atattaacgt ttacaattt
//
