Basic Vector Information
PBDGTV vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
PBDGTV vector Sequence
LOCUS 40924_6162 7640 bp DNA circular SYN 17-DEC-2018
DEFINITION Cloning vector PBDGTV, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7640)
AUTHORS Liu G, Wang X, Liu Y, Zhang M, Jia Y, Huang Y.
TITLE Arrayed mutant haploid stem cell libraries facilitate
phenotype-driven genetic screens
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7640)
AUTHORS Liu G, Wang X, Liu Y, Zhang M, Jia Y, Huang Y.
TITLE Direct Submission
JOURNAL Submitted (20-NOV-2015) Department of Medical Genetics, Institute of
REFERENCE 3 (bases 1 to 7640)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 7640)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(20-NOV-2015) Department of Medical Genetics, Institute of Basic
Medical Sciences, Chinese Academy of Medical Sciences "
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7640
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 24..128
/label=AmpR promoter
CDS 129..986
/label=AmpR
/note="beta-lactamase"
rep_origin 1160..1748
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 2036..2057
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2072..2102
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2110..2126
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2134..2150
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 2171..2189
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
mobile_element complement(2248..2557)
/mobile_element_type="transposon:PB3'TR"
/label='TR
gene 2569..3292
/gene="Ccdc107"
/label=Ccdc107
exon 2616..2728
/gene="Ccdc107"
/note="penultimate Ccdc107 exon"
exon 2804..2927
/gene="Ccdc107"
/note="final Ccdc107 exon"
promoter 3320..3819
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
regulatory 3896..3905
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 3908..4501
/label=PuroR
/note="puromycin N-acetyltransferase"
CDS 4508..5500
/label=Delta-TK
/note="truncated herpes simplex virus thymidine kinase
(Salomon et al., 1995)"
polyA_signal 5531..5755
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
gene complement(6144..6561)
/gene="Dom3z"
/label=Dom3z
exon complement(6144..6387)
/gene="Dom3z"
/note="final Dom3z exon"
exon complement(6467..6561)
/gene="Dom3z"
/note="penultimate Dom3z exon"
mobile_element 6686..6920
/mobile_element_type="transposon:PB5'TR"
/label='TR
promoter complement(7000..7018)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(7025..7041)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 7183..7638
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
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