LOCUS       40924_6162        7640 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector PBDGTV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7640)
  AUTHORS   Liu G, Wang X, Liu Y, Zhang M, Jia Y, Huang Y.
  TITLE     Arrayed mutant haploid stem cell libraries facilitate 
            phenotype-driven genetic screens
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7640)
  AUTHORS   Liu G, Wang X, Liu Y, Zhang M, Jia Y, Huang Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-NOV-2015) Department of Medical Genetics, Institute of
REFERENCE   3  (bases 1 to 7640)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7640)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-NOV-2015) Department of Medical Genetics, Institute of Basic 
            Medical Sciences, Chinese Academy of Medical Sciences "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7640
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /label=AmpR promoter
     CDS             129..986
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1160..1748
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2072..2102
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     mobile_element  complement(2248..2557)
                     /mobile_element_type="transposon:PB3'TR"
                     /label='TR
     gene            2569..3292
                     /gene="Ccdc107"
                     /label=Ccdc107
     exon            2616..2728
                     /gene="Ccdc107"
                     /note="penultimate Ccdc107 exon"
     exon            2804..2927
                     /gene="Ccdc107"
                     /note="final Ccdc107 exon"
     promoter        3320..3819
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     regulatory      3896..3905
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3908..4501
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     CDS             4508..5500
                     /label=Delta-TK
                     /note="truncated herpes simplex virus thymidine kinase
                     (Salomon et al., 1995)"
     polyA_signal    5531..5755
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     gene            complement(6144..6561)
                     /gene="Dom3z"
                     /label=Dom3z
     exon            complement(6144..6387)
                     /gene="Dom3z"
                     /note="final Dom3z exon"
     exon            complement(6467..6561)
                     /gene="Dom3z"
                     /note="penultimate Dom3z exon"
     mobile_element  6686..6920
                     /mobile_element_type="transposon:PB5'TR"
                     /label='TR
     promoter        complement(7000..7018)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7025..7041)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      7183..7638
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagctcga aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggaaagt
     2221 ttaggtcgag taaagcgcaa atctttttta accctagaaa gatagtctgc gtaaaattga
     2281 cgcatgcatt cttgaaatat tgctctctct ttctaaatag cgcgaatccg tcgctgtgca
     2341 tttaggacat ctcagtcgcc gcttggagct cccgtgaggc gtgcttgtca atgcggtaag
     2401 tgtcactgat tttgaactat aacgaccgcg tgagtcaaaa tgacgcatga ttatctttta
     2461 cgtgactttt aagatttaac tcatacgata attatattgt tatttcatgt tctacttacg
     2521 tgataactta ttatatatat attttcttgt tatagatgcc taccggccag ccaaggagac
     2581 agactgggca gccccctgag tgggtatttc tacagggtga cgacttaggt tgaaacccat
     2641 atggaacttc tagacacaaa actaaagacc atccaccacc ttctacaaga ctgccagcct
     2701 ggcacaggag tggaagttcc agaaccaggt aaaaggttgg gaggtttcta tggctggaga
     2761 gccatggctg tgaaactaga gcaagttggt ctctccttca cagaggccag catacccttt
     2821 actgaggacc tggggaaaga agaccaagaa gctggaaaca gtcaggcctg ggaagagccc
     2881 ataacctgga gcccagaaac acggaaccta gctccttcct gggaggtgga gcaggggctg
     2941 aggagaaggt ggcacaagac ggtgacaaag ggtccagcag taaatgggga acaaccactg
     3001 aaggtttagt aaaaagagtc ccctcgtact gggtgttcct ctgtgctttt tccactccct
     3061 gctacacaca cacacacggt gcaaaagcca agcactcttc ccttattagg aagagagaga
     3121 ctgccttcca gaatgtgaat ggagaagaca gagagagagc cacagttctg cccttgtgtt
     3181 tctgaggcta ccgagatgcc agccatcaag gctcaaactc cattttctcg tgatttagac
     3241 aggaaggctt ttgcaaaaac aaccctttat taagatccag tggggaaata aaggagcttg
     3301 atatcgaatt aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
     3361 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat
     3421 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
     3481 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
     3541 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
     3601 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
     3661 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg
     3721 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
     3781 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagata acttcgtata
     3841 gcatacatta tacgaagcgg gggggcccgg tacggatcct cgagcagctg ccaacgccac
     3901 catggggacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc
     3961 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc
     4021 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     4081 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     4141 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     4201 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     4261 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     4321 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     4381 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     4441 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     4501 cggatccatg cccacgctac tgcgggttta tatagacggt cctcacggga tggggaaaac
     4561 caccaccacg caactgctgg tggccctggg ttcgcgcgac gatatcgtct acgtacccga
     4621 gccgatgact tactggcagg tgctgggggc ttccgagaca atcgcgaaca tctacaccac
     4681 acaacaccgc ctcgaccagg gtgagatatc ggccggggac gcggcggtgg taatgacaag
     4741 cgcccagata acaatgggca tgccttatgc cgtgaccgac gccgttctgg ctcctcatat
     4801 cgggggggag gctgggagct cacatgcccc gcccccggcc ctcaccctca tcttcgaccg
     4861 ccatcccatc gccgccctcc tgtgctaccc ggccgcgcga taccttatgg gcagcatgac
     4921 cccccaggcc gtgctggcgt tcgtggccct catcccgccg accttgcccg gcacaaacat
     4981 cgtgttgggg gcccttccgg aggacagaca catcgaccgc ctggccaaac gccagcgccc
     5041 cggcgagcgg cttgacctgg ctatgctggc cgcgattcgc cgcgtttacg ggctgcttgc
     5101 caatacggtg cggtatctgc agggcggcgg gtcgtggcgg gaggattggg gacagctttc
     5161 ggggacggcc gtgccgcccc agggtgccga gccccagagc aacgcgggcc cacgacccca
     5221 tatcggggac acgttattta ccctgtttcg ggcccccgag ttgctggccc ccaacggcga
     5281 cctgtacaac gtgtttgcct gggccttgga cgtcttggcc aaacgcctcc gtcccatgca
     5341 cgtctttatc ctggattacg accaatcgcc cgccggctgc cgggacgccc tgctgcaact
     5401 tacctccggg atggtccaga cccacgtcac cacccccggc tccataccga cgatctgcga
     5461 cctggcgcgc acgtttgccc gggagatggg ggaggctaac tgagctctag agctcgctga
     5521 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     5581 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     5641 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     5701 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     5761 gaggcggaaa gaaccagctg gggctcgaga tccactagtt ctagcctcga gcaagtcttt
     5821 cagtcctata gaaataaaat caggaattaa aaagaagatt ctgaatattt atcttttata
     5881 acaaatactt cttacacatt aaacttttac aggacacatt ccttgtctct atgaagggaa
     5941 ttcgcgccgc cagcctctac acccagtaaa ggatgtgtgg atagaaatgt aacaataagc
     6001 tgggttgtag tagcagcgaa ggggcagata aatctctttg agtttgatgc caaccatgtt
     6061 ttgaaaaaac taaaaacaaa caaaaaaaac cccctaaaaa cccaaaaata taaataaaga
     6121 gcaaaccaga gtgcttggag tatggtaact tggaacagag gtgactccat tcagacacag
     6181 caagagaccc acttagaaac acgcctttat tatctgcacg gagcccagac ttgtcctcag
     6241 tccttgggag agggagtctt tgagagtggt gggaggtctt gggtcatagt ctccacatac
     6301 catgagggca ggaaggcata gggtgcgtca cgatggacag acacagtgac agggccacca
     6361 ggttcccagg agaagaggtg gacaagcctg tggaagacag aggtgtgggg tgaagccaga
     6421 gattgagccg ggatccagag gcagtgcaca aacatgaagg ccttacctgg gatcgtcctg
     6481 gactactgtg ctctgggcaa agctaaggaa ggcagcacag aagttcatgc acacagaggg
     6541 aattcagcct tctcggtcat tctggagggg gcacaagagg cagaagactg ggggtggaaa
     6601 gaggaaaagg caggtggcaa aggggcactg ggtctgagga cttggggccg gccggatttt
     6661 tgtaaaagag agaatgttta aaagttttgt tactttatag aagaaatttt gagtttttgt
     6721 ttttttttaa taaataaata aacataaata aattgtttgt tgaatttatt attagtatgt
     6781 aagtgtaaat ataataaaac ttaatatcta ttcaaattaa taaataaacc tcgatataca
     6841 gaccgataaa acacatgcgt caattttacg catgattatc tttaacgtac gtcacaatat
     6901 gattatcttt ctagggttaa ataatagttt ctaatttttt tattattcag gcgaagttac
     6961 atgtatcgat ctcgaggggg ggcccggtac ccaattcgcc ctatagtgag tcgtattaca
     7021 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     7081 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     7141 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     7201 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     7261 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     7321 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     7381 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     7441 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     7501 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     7561 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     7621 tattaacgct tacaatttag
//
