pBbEB1c-RFP vector (V009270)

LOCUS       40924_5969        6808 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pBbEB1c-RFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6808)
  AUTHORS   Kang MK, Lee J, Um Y, Lee TS, Bott M, Park SJ, Woo HM.
  TITLE     Synthetic biology platform of CoryneBrick vectors for gene 
            expression in Corynebacterium glutamicum and its application to 
            xylose utilization
  JOURNAL   Appl. Microbiol. Biotechnol. 98 (13), 5991-6002 (2014)
  PUBMED    24706215
REFERENCE   2  (bases 1 to 6808)
  AUTHORS   Woo HM.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JAN-2014) Clean Energy Research Center, Korea 
            Institute of Science and Technology, Hwarangno 14-gil 5, 
            Seongbuk-gu, Seoul, Seoul 136-791, Korea
REFERENCE   3  (bases 1 to 6808)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6808)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Microbiol. Biotechnol."; date: "2014"; volume: "98"; issue: "13"; 
            pages: "5991-6002"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-JAN-2014) Clean Energy Research Center, Korea Institute of 
            Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul, 
            Seoul 136-791, Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..84
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             85..1164
                     /label=lacI
                     /note="lac repressor"
     promoter        1399..1428
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    1436..1452
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1493..2167
                     /label=mRFP1
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
     terminator      2203..2274
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2290..2317
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      2477..5031
     rep_origin      complement(5232..5820)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(5908..6002)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(6026..6682)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(6683..6785)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"