LOCUS       40924_5969        6808 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pBbEB1c-RFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6808)
  AUTHORS   Kang MK, Lee J, Um Y, Lee TS, Bott M, Park SJ, Woo HM.
  TITLE     Synthetic biology platform of CoryneBrick vectors for gene 
            expression in Corynebacterium glutamicum and its application to 
            xylose utilization
  JOURNAL   Appl. Microbiol. Biotechnol. 98 (13), 5991-6002 (2014)
  PUBMED    24706215
REFERENCE   2  (bases 1 to 6808)
  AUTHORS   Woo HM.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JAN-2014) Clean Energy Research Center, Korea 
            Institute of Science and Technology, Hwarangno 14-gil 5, 
            Seongbuk-gu, Seoul, Seoul 136-791, Korea
REFERENCE   3  (bases 1 to 6808)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6808)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Microbiol. Biotechnol."; date: "2014"; volume: "98"; issue: "13"; 
            pages: "5991-6002"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-JAN-2014) Clean Energy Research Center, Korea Institute of 
            Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul, 
            Seoul 136-791, Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..84
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             85..1164
                     /label=lacI
                     /note="lac repressor"
     promoter        1399..1428
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    1436..1452
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1493..2167
                     /label=mRFP1
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
     terminator      2203..2274
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2290..2317
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      2477..5031
     rep_origin      complement(5232..5820)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(5908..6002)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(6026..6682)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(6683..6785)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gacgtcgaca ccatcgaatg gtgcaaaacc tttcgcggta tggcatgata gcgcccggaa
       61 gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat
      121 gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg
      181 aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg
      241 gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc
      301 ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc
      361 agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac
      421 aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat
      481 gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac
      541 cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag
      601 catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc
      661 tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg
      721 atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg
      781 ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc
      841 gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga
      901 tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat
      961 tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg
     1021 gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc
     1081 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     1141 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt agcgcgaatt
     1201 gatctggttt gacagcttat catcgactgc acggtgcacc aatgcttctg gcgtcaggca
     1261 gccatcggaa gctgtggtat ggctgtgcag gtcgtaaatc actgcataat tcgtgtcgct
     1321 caaggcgcac tcccgttctg gataatgttt tttgcgccga catcataacg gttctggcaa
     1381 atattctgaa atgagctgtt gacaattaat catccggctc gtataatgtg tggaattgtg
     1441 agcggataac aatttcagaa ttcaaaagat cttttaagaa ggagatatac atatggcgag
     1501 tagcgaagac gttatcaaag agttcatgcg tttcaaagtt cgtatggaag gttccgttaa
     1561 cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac
     1621 cgctaaactg aaagttacca aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc
     1681 gcagttccag tacggttcca aagcttacgt taaacacccg gctgacatcc cggactacct
     1741 gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg
     1801 tgttgttacc gttacccagg actcctccct gcaagacggt gagttcatct acaaagttaa
     1861 actgcgtggt accaacttcc cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg
     1921 ggaagcttcc accgaacgta tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat
     1981 gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca cctacatggc
     2041 taaaaaaccg gttcagctgc cgggtgctta caaaaccgac atcaaactgg acatcacctc
     2101 ccacaacgaa gactacacca tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac
     2161 cggtgcttaa ggatccaaac tcgagtaagg atctccaggc atcaaataaa acgaaaggct
     2221 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     2281 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atacctaggg cgttcggctg
     2341 cggcgagcgg tatcagcagt tattggtgcc cttcgaaatg accgaccaag cgacgcccaa
     2401 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     2461 cgttttccgg gacgccaaca acaagaccca tcatagtttg cccccgcgac attgaccata
     2521 aattcatcgc acaaaatatc gaacggggtt tatgccgctt ttagtgggtg cgaagaatag
     2581 tctgctcatt acccgcgaac accgccgcat tcagatcacg cttagtagcg tccccatgag
     2641 taggcagaac cgcgtccaag tccacatcat ccataacgat catgcacggg gtggaatcca
     2701 cacccagact tgccagcacc tcattagcga cacgttgcgc agcggccacg tccttagcct
     2761 tatccacgca atcgagaacg tactgcctaa ccgcgaaatc agactgaatc agtttccaat
     2821 catcgggctt caccaaagca acagcaacgc gggttgattc gacccgttcc ggtgcttcca
     2881 gaccggcgag cttgtacagt tcttcttcca tttcacgacg tacatcagcg tctatgtaat
     2941 caatgcccaa agcacgctta gccccacgtg accaggacga acgcaggttt ttagaaccaa
     3001 cctcatactc acgccaccga gccaccaaaa cagcgtccat atcctcgccg gcgtcgcttt
     3061 gatcggccaa catatccaac atctgaaacg gcgtgtacga ccccttagac gcggttttag
     3121 tagcggagcc agtcagttcc tgagacatgc ccttagcgag gtaggttgcc attttcgcag
     3181 cgtctccacc ccaggtagac acctgatcaa gtttgacccc gtgctcacgc agtggcgcgt
     3241 ccataccggc cttaaccaca ccagcagacc agcgggaaaa catggaatcc tcaaacgcct
     3301 tgagttcatc gtcagacagt ggacgatcca agaacaacag catgttgcgg tgcaagtgcc
     3361 aaccgttcgc ccaagagtct gtgacctcat agtcactata ggtgtgctcc accccgtacc
     3421 gtgcacgttc tttcttccac tgagatgttt tcaccatcga agagtacgca gtcttaatac
     3481 ccgcttcaac ctgcgcaaat gactgtgagc ggttgtgtcg aacagtgccc acaaacatca
     3541 tgagcgcgcc acccgccgcc aagtgattct tagtagcaat agccagctca atgcggcgtt
     3601 cgcccatgac ttccaattca gccagaggtg acccccagcg agagtgagag ttttgcagac
     3661 cctcaaactg cgaagcaccg ttagacgacc aggacaccgc aacagcttcg tccctgcgcc
     3721 acctatggca ccccgccaga gccttactat tggtgatctt gtacatgacg ttttgcctac
     3781 gccacgccct agcgcgagtg accttagaac cctcattgac ctgcggttcc ttagaggtgt
     3841 tcacttctat ttcagtgtta ctcagtgtta cctagacccg atgttgtgcg gggttgcgca
     3901 gtgcgagttt gtgcgggtgt tgtgcccgtt gtcttagcta gtgctatggt tgtcaattga
     3961 aaccccttcg ggttatgtgg cccccgtgca tatgagttgg tagctcgcac gggggtttgt
     4021 cttgtctagg gactattaat ttttagtggt gtttggtggc cgcctagctt ggctatgcgt
     4081 gccagcttac ccgtactcaa tgttaaagat ttgcatcgac atgggagggt tacgtgtccg
     4141 atacctaggg ggggtatccg cgactaggtg ccccggtgct cactgtctgt accggcgggg
     4201 caagccccac accccgcatg gacagggtgg ctccgccccc tgcaccccca gcaatctgca
     4261 tgtacatgtt ttacacatta gcacgacatg actgcatgtg catgcactgc atgcagacta
     4321 ggtaaatatg agtatgtacg actagtaaca ggagcactgc acataatgaa tgagttgcag
     4381 gacaatgttt gctacgcatg cgcatgacat atcgcaggaa agctactaga gtcttaaagc
     4441 atggcaacca aggcacagct agaacagcaa ctacaagaag ctcaacaggc actacaggcg
     4501 cagcaagcgc aggcacaagc caccatcgaa gcactagaag cgcaggcaaa ggctaagccc
     4561 gtcgtggtca ccgcacgcgt tcctttggca ctacgtgagg acatgaagcg cgcaggcatg
     4621 cagaacggtg aaaacctcca agagttcatg atcgccgcgt ttaccgagcg gctagaaaag
     4681 ctcaccacca ccgacaacga ggaaaacaat gtctaaccca ctagttctct ttgcccaccg
     4741 tgacccggta aatgacgtga cgttcgagtg cattgagcac gccacctacg acacactttc
     4801 acacgctaaa gaccagatca ccgcccaaat gcaagcccta gacgaagaag ccgccctact
     4861 gccctaatgg gtgtttcatg ggtgtttccc tagtgtttca tggtgttttc acctaagcta
     4921 gggaattgcg cgagaagtct cgcaaaaatc agcaaccccc ggaaccacac agttcacggg
     4981 ggttcttcta tgccagaaat cagaaagggg aaccagtgaa cgaccccgaa tggctggatg
     5041 atcctccagc gcggggatct catgctggag ttcttcgccc accccaaaag gatctaggtg
     5101 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5161 gcgtcagacc ccgtatgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     5221 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     5281 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     5341 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     5401 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     5461 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     5521 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     5581 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     5641 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     5701 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     5761 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     5821 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     5881 gggattttgg tcatgactag tgcttggatt ctcaccaata aaaaacgccc ggcggcaacc
     5941 gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag
     6001 tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag tactgttgta
     6061 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg
     6121 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg
     6181 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat
     6241 tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac
     6301 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
     6361 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
     6421 cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat
     6481 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
     6541 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
     6601 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
     6661 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa
     6721 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
     6781 gatcaacgtc tcattttcgc cagatatc
//
