pBact-Pcad vector (V009307)

LOCUS       V009307                 9104 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009307
VERSION     V009307
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9104)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9104)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9104)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9104)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2196..2471
                     /label="chicken beta-actin promoter"
     misc_feature    2472..3466
                     /label="5' UTR of chicken beta-actin"
                     /note="5' UTR of chicken beta-actin"
     misc_feature    3512..3556
                     /label="5' UTR of mCdh3"
                     /note="5' UTR of mCdh3"
     CDS             3557..6022
                     /gene="Cdh3"
                     /label="Cadherin-3"
                     /note="Cadherin-3 from Mus musculus. Accession#: P10287"
     misc_feature    6026..6698
                     /label="3' UTR of mCdh3"
                     /note="3' UTR of mCdh3"
     polyA_signal    6717..6851
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6883..6899)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6907..6923)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6931..6961)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6976..6997)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7285..7873)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8047..8904)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8905..9009)
                     /label="AmpR promoter"