LOCUS       V009307                 9104 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009307
VERSION     V009307
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9104)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9104)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9104)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9104)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2196..2471
                     /label="chicken beta-actin promoter"
     misc_feature    2472..3466
                     /label="5' UTR of chicken beta-actin"
                     /note="5' UTR of chicken beta-actin"
     misc_feature    3512..3556
                     /label="5' UTR of mCdh3"
                     /note="5' UTR of mCdh3"
     CDS             3557..6022
                     /gene="Cdh3"
                     /label="Cadherin-3"
                     /note="Cadherin-3 from Mus musculus. Accession#: P10287"
     misc_feature    6026..6698
                     /label="3' UTR of mCdh3"
                     /note="3' UTR of mCdh3"
     polyA_signal    6717..6851
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6883..6899)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6907..6923)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6931..6961)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6976..6997)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7285..7873)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8047..8904)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8905..9009)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gttgggatct ttgcattggc
      421 ccacggctct caggatgggg atgctcccct tcagcacccg gttccccttg gaaactgatg
      481 gtcctggctc tgtggcatgg cagtggcact gtgaggagcc ccctaccagc agcacacagt
      541 ggggttggca ctgccacgct ccggatgccg cgctctgatc caaccccata atcaagggaa
      601 cccgaattgc cccatcattg cccccaccac ccccatcctg ccgggccctc acaccccacg
      661 ctgccttctg gtgacattcc ccagcccaaa cccacggctt catggctacc gcggggcatt
      721 tcccattgcc gccccattat cagctctgca cacctcccgc tgtacccatg cctcgtggct
      781 gcccttcttt gacgtataat cttctaatta atacccggcc ttgtcaaagt ggagcacaaa
      841 cgttaattaa ttccccagca ggcaggtaat taacagtgtg actccctttt tgctgcgagt
      901 ggggctgata cagagagatg tggcactatg gagcccacgg ggtcctggca ctgggtgccc
      961 acggaggtcc ccatgtgctg cagtgtcacc gcctccgagg agacagtatt gtccctgcgg
     1021 tgtccctgca gctcagctct gtccacaggg ccacctccag tttggagggg acacaatgca
     1081 gccccgatgc aacccatcct cgcagcatcc cagggacaaa gaccccactg caagaccgca
     1141 cacagggctg ggtcccgctc ccctaatatc tacagtgctt cttgcatggc cccttaatca
     1201 atgcagttaa tcagcatgcg ctcatgcacc gctctggagc tgcaaagccc ctcgcagcgc
     1261 tgctcaccaa caccgcgcac cgcccctgcc cagcctgcag cacgcgctgc aaacaggaaa
     1321 gaaacaaaat attgcccaaa tgtaggcaaa ggcattcggc tgccttgacc tccgccgggc
     1381 cgggccctgc ctgactcagc tccttactca gcgctcgctt cctccctccg gctgccaccg
     1441 ccgcagcgca caccctgaca aagagtggcc cttaacgggc tctgaggtgc acccagcagt
     1501 gcactcagca gtccaagggc cggcctggag gtttgcaccg ctacgtgctg acattagcat
     1561 tgaacttggc cctgggtagt gctgcaggcc gggcggggtg ggtgtagaga gtgcagcgcg
     1621 cgttgcaccc ggtgcccctt cccctccctt gcatcccagc aggctgcacc ccagcaccag
     1681 gcccgtgcat gcatgctcct ggtgttattg cagcctggtg catgcatgcg tcttgatggt
     1741 gcagcgctgt gcatgcatcc tccttggtgt gtagcagtct agtgcatgca tacccctcgg
     1801 tgttattgct gctctgtgca cgcacgctca ttgtatcact tcatcccagt gcatgcactc
     1861 acactggagc gattgctgct cggtgcacgc acactcattg tatcactgca gctcagtggc
     1921 tgcacgcaca ccggtgttat tgctgctcgg tgcgtgcatg cacatcagtg tcgctgcagc
     1981 tcagtgcatg cacgctcatt gcccatcgct atccctgcct ctcctgctgg cgctccccgg
     2041 gaggtgactt caaggggacc gcaggaccac ctcgggggtg gggggagggc tgcacacgcg
     2101 gaccccgctc cccctcccca acaaagcact gtggaatcaa aaagggggga ggggggatgg
     2161 aggggcgcgt cacacccccg ccccacaccc tcacctcgag gtgagcccca cgttctgctt
     2221 cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt
     2281 attttgtgca gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg
     2341 gcgaggggcg gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc
     2401 tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg
     2461 cgcggcgggc gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc
     2521 gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc
     2581 cttctcctcc gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc
     2641 tgcgtgaaag ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg
     2701 ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct
     2761 gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc
     2821 ggccgggggc ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg
     2881 ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc
     2941 cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc
     3001 ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg
     3061 cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc
     3121 gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag
     3181 agggcgcagg gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc
     3241 gcaccccctc tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     3301 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg
     3361 ccgcaggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt
     3421 gtgaccggcg gggtttatat cttcccttct ctgttcctcc gcagccngtc gactctagag
     3481 gatctnnnnn aagcttgata tcgaattcnn ncgagcgcgc cccaccgtcg cggcaccttc
     3541 tcacctccct tcagttatgg agcttcttag tgggcctcac gccttcctgc tcctcctgct
     3601 ccaggtttgc tggctacgca gcgtggtctc cgagccctac cgagcgggct tcatcgggga
     3661 ggctggagtg accttggagg tggaaggaac tgacctggag ccgagccaag ttctggggaa
     3721 agtagccttg gctggacagg gcatgcacca tgcagacaat ggagacatca ttatgctgac
     3781 tagggggaca gttcagggag ggaaggatgc gatgcactcc ccacccaccc gcatcttaag
     3841 gagacgaaag agagagtggg tgatgccacc aatattcgtc cccgagaatg gcaagggtcc
     3901 cttccctcag aggctgaatc agctcaaatc taataaggac agaggcacca agattttcta
     3961 cagcatcaca gggcctggcg cagacagtcc ccccgaagga gtcttcacca tagagaagga
     4021 gtcgggctgg ctgttgttgc atatgccact ggacagggag aagattgtca agtacgagct
     4081 ttatggccac gctgtatctg agaatggtgc ctctgtagag gagcccatga acatatccat
     4141 cattgtgaca gaccagaatg acaacaagcc caagttcact caagacacct tcagagggag
     4201 tgttctggag ggagtaatgc ctggcacttc tgtgatgcag gtgacagcca cagatgagga
     4261 cgatgctgtc aacacttaca atggggtggt ggcttactcc atccatagcc aagagccgaa
     4321 ggagccacac gacctcatgt tcaccatcca taaaagcacg ggaaccatta gcgtcatatc
     4381 cagtggcctg gaccgagaga aagtccctga gtacagactg accgtccagg ccacagacat
     4441 ggatggagag ggctctacca cgacggcaga ggccgttgtg caaatccttg atgccaacga
     4501 taacgctccg gagtttgagc cgcagaagta tgaggcttgg gtgcctgaga acgaagtggg
     4561 ccatgaggta cagaggctga cagtgactga tctcgatgtc cccaactcgc cagcgtggcg
     4621 tgccacctac cacatcgtgg gaggtgatga tggggaccat ttcaccatca ccactcaccc
     4681 agagaccaac caaggcgtcc tgacaaccaa gaagggtttg gattttgagg ctcaggacca
     4741 acacaccctg tatgtagaag tgaccaacga ggctcccttt gcagtgaagc tcccgacagc
     4801 cactgccacc gtggtggtcc atgtgaaaga tgtcaacgaa gcccctgtgt ttgttccacc
     4861 ttccaaggtc attgaggccc aggaaggcat ctctattggg gaactggtct gcatctatac
     4921 cgcacaggac ccagacaagg aggaccagaa gatcagctac accatctcga gagatccagc
     4981 caactggctt gctgtggacc cagacagtgg tcagataact gccgcaggca tcttggatcg
     5041 tgaggacgag cagtttgtga aaaacaatgt ctacgaagtc atggttttgg ccacagacag
     5101 tggaaaccct cccaccaccg gcactgggac cctcctgctt acacttactg acatcaacga
     5161 ccatggcccg atccctgaac ccaggcagat catcatctgt aaccaaagcc ctgtgcctca
     5221 agtgctgaac atcactgaca aggacctgtc ccccaactcc tcccctttcc aggcccagct
     5281 aacacatgac tcagatatct actggatggc agaagtcagc gagaaaggag acaccgtggc
     5341 cttgtccctg aagaagttcc tgaaacaaga cacgtatgac ttgcatcttt ctttgtctga
     5401 ccatggcaac agggaacagc taaccatgat cagggccact gtgtgtgact gccatggcca
     5461 agtgttcaat gactgcccca gaccctggaa gggtggtttc atcctcccca tcctgggtgc
     5521 tgtcctggca ctgctgaccc ttctactggc actcctcctg ttggtgagga agaagaggaa
     5581 ggtcaaagag ccccttctgc tcccagaaga tgacacgcga gacaatgtct tctattatgg
     5641 agaagagggt ggtggtgaag aggaccagga ctatgacatc acccaactcc accggggact
     5701 ggaggccagg cctgaggtgg ttctccgaaa cgatgtagtg ccaaccttca tccccacccc
     5761 catgtaccga ccccggcccg ccaacccaga tgaaatcggg aacttcatca tcgagaacct
     5821 gaaggctgcc aacactgacc ctactgcccc gccctacgac tccctgctgg tttttgacta
     5881 cgagggcagc ggctctgatg ccgcctccct gagctccctc accacctccg cctccgacca
     5941 ggatcaggac tacaactacc ttaacgagtg gggaagtcga ttcaagaaac tggcggacat
     6001 gtatggtggc ggtgaggatg actaggctgt ccctgccaag ctgtccaggc aagaactaca
     6061 gacgggtcac agcagcatct caagggagcc tggctcccgc tccagccagg actctgaagt
     6121 ttgcccagaa gtggcctgcg tggcacgctg catctggacc agaaggctgg actgtgagcc
     6181 tttccgatgg aaggatgtga ctctgacgac aacatgatcc ccacctcagc catggctgag
     6241 cccctctcag agcgggaatg tctaaaggtc tcccgcctgc cacacaacac tcagctctgc
     6301 cctctcaggg cttgaacttg ctccgatgcc ctcggtgact ttccccactt ggaatggctc
     6361 cctctcttta gaaagaggcc cctaactaag gccctgtttt tctttaatgc agttttcaaa
     6421 aagaggctgg ggtgggtggg gcagggtgcg gacatcagtg tcccctcagg ccctccagaa
     6481 gagaagccta cagggcactg gccctggtac atttctctga catttcttga ggcctcacat
     6541 tggggagggg tcactgcctt tttttttttt ttaatactga gtatatctaa gttgtctcgt
     6601 atttttttat tttccctgtt atgtgctgta gatggagagt gatgacaatc gtgtaaatgt
     6661 actagaattt ttttattaaa atgaactttt ttttttcccc cgaattcctg cagcccaact
     6721 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6781 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6841 atgtctggat ccccgggtac cgagctcgaa ttcgtaatca tggtcatagc tgtttcctgt
     6901 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     6961 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     7021 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     7081 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7141 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7201 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7261 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7321 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7381 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7441 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7501 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7561 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7621 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7681 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     7741 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7801 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7861 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7921 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7981 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     8041 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     8101 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     8161 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     8221 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     8281 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     8341 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     8401 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     8461 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     8521 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     8581 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     8641 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     8701 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     8761 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     8821 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     8881 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     8941 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     9001 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     9061 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc
//
