pBacPAK-His2 vector (V009314)

LOCUS       40924_5664        5415 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Baculovirus transfer vector pBacPAK-His2, complete sequence, 
            beta-lactamase (bla) gene, complete cds.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Kitts PA.
  TITLE     pBacPAK-His2 transfer vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   Kitts PA.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-1996) Paul A. Kitts, CLONTECH Laboratories, Inc., 
            1020 East Meadow Circle, Palo Alto, CA 94303-4230, USA
REFERENCE   3  (bases 1 to 5415)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5415)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-APR-1996) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East 
            Meadow Circle, Palo Alto, CA 94303-4230, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     This vector can be obtained from CLONTECH Laboratories, Inc., 1020 
            East  Meadow Circle, Palo Alto, CA 94303-4230, USA. To place an 
            order call  (415) 424-8222 or (800) 662-2566, extension 1. 
            International customers,  please contact your local distributor. For
            technical information, call  (415) 424-8222 or (800) 662-2566, 
            extension 3.
            This sequence has been compiled from information in the sequence 
            databases,  published literature and other sources, together with 
            partial sequences  obtained by CLONTECH. If you suspect there is an 
            error in this sequence,  please contact CLONTECH's Technical Service
            Department at (415) 424-8222 or  (800) 662-2566, extension 3 or 
            E-mail TECH@CLONTECH.COM.
FEATURES             Location/Qualifiers
     source          1..5415
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     223..1050
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     promoter        1054..1145
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             1155..1172
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     misc_recomb     1274..2631
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      2778..3233
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3515..3619
                     /label=AmpR promoter
     CDS             3620..4477
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4651..5239
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"