LOCUS       40924_5664        5415 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Baculovirus transfer vector pBacPAK-His2, complete sequence, 
            beta-lactamase (bla) gene, complete cds.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Kitts PA.
  TITLE     pBacPAK-His2 transfer vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   Kitts PA.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-1996) Paul A. Kitts, CLONTECH Laboratories, Inc., 
            1020 East Meadow Circle, Palo Alto, CA 94303-4230, USA
REFERENCE   3  (bases 1 to 5415)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5415)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-APR-1996) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East 
            Meadow Circle, Palo Alto, CA 94303-4230, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     This vector can be obtained from CLONTECH Laboratories, Inc., 1020 
            East  Meadow Circle, Palo Alto, CA 94303-4230, USA. To place an 
            order call  (415) 424-8222 or (800) 662-2566, extension 1. 
            International customers,  please contact your local distributor. For
            technical information, call  (415) 424-8222 or (800) 662-2566, 
            extension 3.
            This sequence has been compiled from information in the sequence 
            databases,  published literature and other sources, together with 
            partial sequences  obtained by CLONTECH. If you suspect there is an 
            error in this sequence,  please contact CLONTECH's Technical Service
            Department at (415) 424-8222 or  (800) 662-2566, extension 3 or 
            E-mail TECH@CLONTECH.COM.
FEATURES             Location/Qualifiers
     source          1..5415
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     223..1050
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     promoter        1054..1145
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             1155..1172
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     misc_recomb     1274..2631
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      2778..3233
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3515..3619
                     /label=AmpR promoter
     CDS             3620..4477
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4651..5239
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tcgacatgct gaacaacaag attaatatgc ctccgtgtat aaaaaaaata ttgaacgatt
       61 tgaaagaaaa caatgtaccg cgcggcggta tgtacaggaa gaggtttata ctaaactgtt
      121 acattgcaaa cgtggtttcg tgtgccaagt gtgaaaaccg atgtttaatc aaggctctga
      181 cgcatttcta caaccacgac tccaagtgtg tcggtgaagt catgcacctt ttaatcaaat
      241 cccaagatgt gtataaacca ccaaactgcc aaaaaatgaa aactgtcgat aagctctgtc
      301 cgtttgctgg caactgcaag ggtctcaatc ctatttgtaa ttattgaata ataaaacaat
      361 tataaatgct aaatttgttt tttattaacg atacaaacca aacgcaacaa gaacatttgt
      421 agtattatct ataattgaaa aagcgtagtt ataatcgctg aggtaatatt taaaatcatt
      481 ttcaaatgat tcacagttaa tttgcgacaa tataatttta ttttcacata aactagacgc
      541 cttgtcgtct tcttcttcgt attccttctc tttttcattt ttctcctcat aaaaattaac
      601 atagttatta tcgtatccat atatgtatct atcgtataga gtaaattttt tgttgtcata
      661 aatatatatg tcttttttaa tggggtgtat agtaccgctg cgcatagttt ttctgtaatt
      721 tacaacagtg ctattttctg gtagttcttc ggagtgtgtt gctttaatta ttaaatttat
      781 ataatcaatg aatttgggat cgtcggtttt gtacaatatg ttgccggcat agtacgcagc
      841 ttcttctagt tcaattacac cattttttag cagcaccgga ttaacataac tttccaaaat
      901 gttgtacgaa ccgttaaaca aaaacagttc acctcccttt tctatactat tgtctgcgag
      961 cagttgtttg ttgttaaaaa taacagccat tgtaatgaga cgcacaaact aatatcacaa
     1021 actggaaatg tctatcaata tatagttgct gatatcatgg agataattaa aatgataacc
     1081 atctcgcaaa taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta
     1141 taaataccat gggtcatcat catcaccatc acggtggtcg acaagcttgg atccctgcag
     1201 gcctcgagtt cgaatctaga agatctggta ccgagctcga attcccgggc ggccgcttaa
     1261 ttaattgatc cgggttatta gtacatttat taagcgctag attctgtgcg ttgttgattt
     1321 acagacaatt gttgtacgta ttttaataat tcattaaatt tataatcttt agggtggtat
     1381 gttagagcga aaatcaaatg attttcagcg tctttatatc tgaatttaaa tattaaatcc
     1441 tcaatagatt tgtaaaatag gtttcgatta gtttcaaaca agggttgttt ttccgaaccg
     1501 atggctggac tatctaatgg attttcgctc aacgccacaa aacttgccaa atcttgtagc
     1561 agcaatctag ctttgtcgat attcgtttgt gttttgtttt gtaataaagg ttcgacgtcg
     1621 ttcaaaatat tatgcgcttt tgtatttctt tcatcactgt cgttagtgta caattgactc
     1681 gacgtaaaca cgttaaataa cgcttggaca tatttaacat cgggcgtgtt agctttatta
     1741 ggccgattat cgtcgtcgtc ccaaccctcg tcgttagaag ttgcttccga agacgatttt
     1801 gccatagcca cacgacgcct attaattgtg tcggctaaca cgtccgcgat caaatttgta
     1861 gttgagcttt ttggaattat ttctgattgc gggcgttttt gggcgggttt caatctaact
     1921 gtgcccgatt ttaattcaga caacacgtta gaaagcgatg gtgcaggcgg tggtaacatt
     1981 tcagacggca aatctactaa tggcggcggt ggtggagctg atgataaatc taccatcggt
     2041 ggaggcgcag gcggggctgg cggcggaggc ggaggcggag gtggtggcgg tgatgcagac
     2101 ggcggtttag gctcaaatgt ctctttaggc aacacagtcg gcacctcaac tattgtactg
     2161 gtttcgggcg ccgtttttgg tttgaccggt ctgagacgag tgcgattttt ttcgtttcta
     2221 atagcttcca acaattgttg tctgtcgtct aaaggtgcag cgggttgagg ttccgtcggc
     2281 attggtggag cgggcggcaa ttcagacatc gatggtggtg gtggtggtgg aggcgctgga
     2341 atgttaggca cgggagaagg tggtggcggc ggtgccgccg gtataatttg ttctggttta
     2401 gtttgttcgc gcacgattgt gggcaccggc gcaggcgccg ctggctgcac aacggaaggt
     2461 cgtctgcttc gaggcagcgc ttggggtggt ggcaattcaa tattataatt ggaatacaaa
     2521 tcgtaaaaat ctgctataag cattgtaatt tcgctatcgt ttaccgtgcc gatatttaac
     2581 aaccgctcaa tgtaagcaat tgtattgtaa agagattgtc tcaagctcgg atcgatcccg
     2641 cacgccgata acaagccttt tcatttttac tacagcattg tagtggcgag acacttcgct
     2701 gtcgtcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
     2761 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     2821 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     2881 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     2941 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
     3001 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     3061 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg
     3121 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     3181 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
     3241 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     3301 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     3361 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     3421 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     3481 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     3541 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     3601 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     3661 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     3721 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     3781 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     3841 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     3901 ctattctcag aatgacttgg ttgaatattc accagtcaca gaaaagcatc ttacggatgg
     3961 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4021 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     4081 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     4141 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     4201 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     4261 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     4321 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     4381 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     4441 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     4501 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     4561 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     4621 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     4681 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     4741 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     4801 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     4861 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     4921 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     4981 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5041 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5101 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     5161 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     5221 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     5281 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     5341 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     5401 agtgagcgag gaagc
//
