pBacb1EG-irEG vector (V009324)

LOCUS       40924_5584        8289 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBacb1EG-irEG, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8289)
  AUTHORS   Naik NG, Lo Y-W., Wu T-Y., Lin C-C., Kuo S-C., Chao Y-C.
  TITLE     Baculovirus as an efficient vector for gene delivery into mosquitoes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8289)
  AUTHORS   Naik NG, Lo Y-W., Wu T-Y., Lin C-C., Kuo S-C., Chao Y-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2018) Institute of Molecular Biology, Academia 
            Sinica, Nankang, Taipei 115, Taiwan
REFERENCE   3  (bases 1 to 8289)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8289)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-APR-2018) Institute of Molecular Biology, Academia Sinica, 
            Nankang, Taipei 115, Taiwan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..95
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     regulatory      138..1997
                     /label=b1
                     /note="b1"
                     /regulatory_class="promoter"
     CDS             2050..2766
                     /label=EmGFP
                     /note="Emerald GFP"
     CDS             3395..4111
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    4146..4369
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    4468..4600
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_recomb     4613..5318
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     promoter        5894..5998
                     /label=AmpR promoter
     CDS             5999..6856
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7030..7618
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"