LOCUS       40924_5584        8289 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBacb1EG-irEG, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8289)
  AUTHORS   Naik NG, Lo Y-W., Wu T-Y., Lin C-C., Kuo S-C., Chao Y-C.
  TITLE     Baculovirus as an efficient vector for gene delivery into mosquitoes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8289)
  AUTHORS   Naik NG, Lo Y-W., Wu T-Y., Lin C-C., Kuo S-C., Chao Y-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2018) Institute of Molecular Biology, Academia 
            Sinica, Nankang, Taipei 115, Taiwan
REFERENCE   3  (bases 1 to 8289)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8289)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-APR-2018) Institute of Molecular Biology, Academia Sinica, 
            Nankang, Taipei 115, Taiwan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..95
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     regulatory      138..1997
                     /label=b1
                     /note="b1"
                     /regulatory_class="promoter"
     CDS             2050..2766
                     /label=EmGFP
                     /note="Emerald GFP"
     CDS             3395..4111
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    4146..4369
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    4468..4600
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_recomb     4613..5318
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     promoter        5894..5998
                     /label=AmpR promoter
     CDS             5999..6856
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7030..7618
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gatatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
       61 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
      121 ccatcgggcg tgctagcgcc tacactcaaa tgttcatcaa tgggttatcc aacgaaagaa
      181 aaaatgaaga gaaatcagcc ttacaagtcc atacgcttga gattcgcatg tcgtcaacaa
      241 ctattcagca gtaaattttg tcacatagga cttagatatc agaatatagt tttcatatat
      301 atgacaaact aattcgtgat tcattaattt ggttaagctt atttgaaaca gctgttccaa
      361 cgcaataaac taacgcggta ttctcaatcc aaacggtaca ccagttatgt gattcccaat
      421 ttcccagagc aattgcagac tcccatagta aaatgctcat tcattcatca tcacacacgt
      481 tccgctgaat ggtagacaac agtggcgcca aaacaaaaac agagcgaggt tcataaatgg
      541 cagctgcgcg ctctcgactc gacgagcagc gcgagctaag tggaattcac cgacggcagc
      601 caaactgatt agcttctaaa aaagtcaaac ttttcatatt ttattgaatg gtaaccaaca
      661 aaaagattgg aaataagcaa aacccttgca attaaaaatt gaaaccacgc acttaaaaat
      721 aaaacaagca gaataagtaa taagctttga actatataaa aaacggttaa aaacaacttt
      781 aagtaagtga atatgtatga tttcaattat cagctttcaa atacagtaat ccgggtcaat
      841 ttgatcacta ttttataact ttttgctgtg ttgtccttcg gaattcaatt attgtcaaat
      901 aaatttcggc aaaaccagaa ctccattgat ctttatctgt ccatgtaatc gatttttcat
      961 gaaataaaat ttaacatact ataaaaatag ttttaatatc caaaattaga atgacgcact
     1021 ggagcgaaat tgatcatcat ataaaaaagc aggtttaaga gtgaaaattt ccctatctac
     1081 taaatttggg tctcctgaat cagagtatga tgtcaaaatc ttacaactct agtatttgat
     1141 catattattg caaaatcaaa ctttgaaaat ctgcctaata cgcctaaata taggcaattt
     1201 cccttaaaat aagcacatta tttgagaata aacgatttta tgagcaaaac actatacttt
     1261 ttatactata tgatatcaaa aatgagttta gcagttcata ctaaaggtaa cacaacattt
     1321 taaacactta tagtttacat gtaggcatgg caccagtgga ttgtttagca tcgacatttc
     1381 caactgtatt gcttacacct tcaaaaattg tgaatattta taagcaaaac tgacacttat
     1441 taaaatgaaa tagtttcaaa tcatcattag gcacctataa acttgaaaac atggaatgtg
     1501 tttggtgcca acaaaccctt cagcatcctt gggaggaaat gtttttcggt accgacctga
     1561 tcggattcgc cccagtgatc aatttgcccc cggttaacgg tagcattttt tgttattctt
     1621 atttttttat aatgtagttt ttaattgcga atttttcatt gcaatttttt gcgaattttt
     1681 cgatgaaaaa atattttgaa atgctgtttt tttttttaat aacaaatttc tactgagctc
     1741 tattttttag ttattgtatt gactgctaaa ggctaagcct ctgatggtta tccttttctt
     1801 agggcatgag aacatttttc ccgttcaccg atcgttggtg cggtgatcgc ctgctcggtg
     1861 attcccggtc gtctttggtc gcgcctccct ttctttacgc aaataaagga ccaggcaagg
     1921 actataaaac caagtcactc caagcaaatc ggttatcagt tagcaaacag cgaaccgtaa
     1981 gtcacctatt ccagtgaaga tctgcgatct aagtaagctt ggcattccgg tactgttggt
     2041 aaagccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     2101 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     2161 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     2221 tggcccaccc tcgtgaccac cttgacctac ggcgtgcagt gcttcgcccg ctaccccgac
     2281 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     2341 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     2401 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2461 ctggggcaca agctggagta caactacaac agccacaagg tctatatcac cgccgacaag
     2521 cagaagaacg gcatcaaggt gaacttcaag acccgccaca acatcgagga cggcagcgtg
     2581 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     2641 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     2701 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     2761 tacaagtaag cggccgctcg agtctagaat tcgataaaag aacctataat cccttcgcac
     2821 accgcgtcac accgcgctat atgctgctca ttaggaatta cggctccttt tttgtggata
     2881 caatctcttg tatacgatat acttattgtt aatttcattg acctttacgc aatcctgcgt
     2941 aaatgctggt atagggtgta cttcggattt ccgagcctat attggttttg aaaggacctt
     3001 taagtcccta ctatactaca ttgtactagc gtaggccacg taggcccgta agatattata
     3061 actattttat tatattttat tcacccccca cattaatccc agttaaagct ttataactat
     3121 aagtaagccg tgccgaaacg ttaatcggtc gctagttgcg taacaactgt tagtttaatt
     3181 ttccaaaatt tatttttcac aatttttagt taagatttta gcttgcctta agcagtcttt
     3241 atatcttctg tatattattt taaagtttat aggagcaaag ttcgctttac tcgcaatagc
     3301 tattttattt attttaggaa tattatcacc tcgtaattat ttaattataa cattagcttt
     3361 atctatttat acccgggatc caccggtcgc caccatggtg agcaagggcg aggagctgtt
     3421 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     3481 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     3541 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     3601 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     3661 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     3721 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     3781 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     3841 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     3901 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     3961 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     4021 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     4081 gatcactctc ggcatggacg agctgtacaa gtaaagcggc cctagagctc gctgatcagc
     4141 ctcgactgtg cctctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     4201 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     4261 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     4321 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     4381 gaaagaacca gctggggctc gagtgcattc tagttgtggt ttgtccaaac tcatcaatgt
     4441 atcttatcat gtctgtatac cgtcgacaac ttgtttattg cagcttataa tggttacaaa
     4501 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4561 ggtttgtcca aactcatcaa tgtatcttat catctctcga ctctgctgaa gaggaggaaa
     4621 ttctccttga agtttccctg gtgttcaaag taaaggagtt tgcaccagac gcacctctgt
     4681 tcactggtcc ggcgtattaa aacacgatac attgttatta gtacatttat taagcgctag
     4741 attctgtgcg ttgttgattt acagacaatt gttgtacgta ttttaataat tcattaaatt
     4801 tataatcttt agggtggtat gttagagcga aaatcaaatg attttcagcg tctttatatc
     4861 tgaatttaaa tattaaatcc tcaatagatt tgtaaaatag gtttcgatta gtttcaaaca
     4921 agggttgttt ttccgaaccg atggctggac tatctaatgg attttcgctc aacgccacaa
     4981 aacttgccaa atcttgtagc agcaatctag ctttgtcgat attcgtttgt gttttgtttt
     5041 gtaataaagg ttcgacgtcg ttcaaaatat tatgcgcttt tgtatttctt tcatcactgt
     5101 cgttagtgta caattgactc gacgtaaaca cgttaaataa agcctggaca tatttaacat
     5161 cgggcgtgtt agctttatta ggccgattat cgtcgtcgtc ccaaccctcg tcgttagaag
     5221 ttgcttccga agacgatttt gccatagcca cacgacgcct attaattgtg tcggctaaca
     5281 cgtccgcgat caaatttgta gttgagcttt ttggaattat ttctgattgc gggcgttttt
     5341 gggcgggttt caatctaact gtgcccgatt ttaattcaga caacacgtta gaaagcgatg
     5401 gtgcaggcgg tggtaacatt tcagacggca aatctactaa tggcggcggt ggtggagctg
     5461 atgataaatc taccatcggt ggaggcgcag gcggggctgg cggcggaggc ggaggcggag
     5521 gtggtggcgg tgatgcagac ggcggtttag gctcaaattg tctctttcag gcaacacagt
     5581 cggcacctca actattgtac tggtttcggg cgtatggtgc actctcagta caatctgctc
     5641 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     5701 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     5761 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     5821 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     5881 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     5941 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     6001 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     6061 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     6121 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     6181 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     6241 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     6301 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     6361 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     6421 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     6481 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     6541 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     6601 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     6661 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     6721 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     6781 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     6841 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     6901 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6961 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     7021 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7081 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7141 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     7201 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7261 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7321 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7381 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7441 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7501 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7561 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7621 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     7681 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     7741 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcatcctg
     7801 caccatcgtc tgctcatcca tgacctgacc atgcagagga tgatgctcgt gacggttaac
     7861 gcctcgaatc agcaacggct tgccgttcag cagcagcaga ccattttcaa tccgcacctc
     7921 gcggaaaccg acatcgcagg cttctgcttc aatcagcgtg ccgtcggcgg tgtgcagttc
     7981 aaccaccgca cgatagagat tcgggatttc ggcgctccac agtttcgggt tttcgacgtt
     8041 cagacgtagt gtgacgcgat cggtataacc accacgctca tcgataattt caccgccgaa
     8101 aggcgcggtg ccgctggcga cctgcgtttc accctgccat aaagaaactg ttacccgtag
     8161 gtagtcacgc aactcgccgc acatctgaac ttcagcctcc agtacagcgc ggctgaaatc
     8221 atcattaaag cgagtggcaa catggaaatc gctgatttgt gtagtcggtt tatgcagcaa
     8281 cgagacgtc
//
