pBac9-PolhEKA vector (V009326)

LOCUS       V009326                 6332 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009326
VERSION     V009326
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6332)
  AUTHORS   Roh JY, Choi JY, Kang JN, Wang Y, Shim HJ, Liu Q, Tao X, Xu HG, Hyun
            JH, Woo SD, Jin BR, Je YH.
  TITLE     Simple purification of a foreign protein using polyhedrin fusion in
            a baculovirus expression system
  JOURNAL   Biosci. Biotechnol. Biochem. 74 (8), 1522-1526 (2010)
   PUBMED   20699589
REFERENCE   2  (bases 1 to 6332)
  AUTHORS   Je YH, Roh JY, Choi JY, Kang JN.
  TITLE     Simple purification of a foreign protein using polyhedrin fusion in
            a baculovirus expression system
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6332)
  AUTHORS   Je YH, Roh JY, Choi JY, Kang JN.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2010) Agricultural Biotechnology, Seoul National
            University, #200 Room 6117, San56-1, Sillim9-dong, Gwanak-gu, Seoul
            151-921, Republic of Korea
REFERENCE   4  (bases 1 to 6332)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6332)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci.
            Biotechnol. Biochem."; date: "2010"; volume: "74"; issue: "8";
            pages: "1522-1526"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (18-FEB-2010) Agricultural Biotechnology, Seoul National University,
            #200 Room 6117, San56-1, Sillim9-dong, Gwanak-gu, Seoul 151-921,
            Republic of Korea"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     327..1154
                     /label="baculovirus recombination region (lef2/ORF603)"
                     /note="contains ORF603 and part of lef2"
     promoter        1158..1249
                     /label="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             1258..1992
                     /gene="PH"
                     /label="Polyhedrin"
                     /note="Polyhedrin from Autographa californica nuclear
                     polyhedrosis virus. Accession#: P04871"
     CDS             2026..2040
                     /label="enterokinase site"
                     /note="enterokinase recognition and cleavage site"
     misc_recomb     2131..3488
                     /label="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     rep_origin      3635..4090
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4372..4476
                     /label="AmpR promoter"
     CDS             4477..5334
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5508..6096
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"